| 基于细菌脱氢酶活性法的水质综合毒性快速测定仪 |
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| 引用本文： | 王立世,张林.基于细菌脱氢酶活性法的水质综合毒性快速测定仪[J].南开大学学报,1998,31(1):100-104. |
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| 作者姓名： | 王立世 张林 |
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| 作者单位： | [1]南工大学环境科学系 [2]军事医学科学院卫生学环境医学研究所 |
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| 摘 要： | 在无氧条件下，生物染料亚甲基蓝可以作为生物新陈代谢过程中体内脱氢酶催化反应的电子受体，亚甲基蓝与细菌悬液共存时，其退色速度标志着细菌脱氢酶活性的大小。脱氢酶的活性大小在一定程度上能够反映出微生物新代谢的能力，微生物所处的水环境中毒物的存在会引起微生物脱氢酶活性变化，毒物的毒性与脱氢酶的活性相关。本文以动力学比色法测定亚甲基蓝的退色速度为基础设计并制造了一台水质综合毒性相关。本文以动力学比色法测定亚
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| 关 键 词： | 细菌 脱氢酶 毒性 水质 监测仪器 |
A NEW INSTRUMENTAL METHOD FOR TOXICITY MONITORING |
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| Wang Lishi, Zhang Baogui, Chen Xulong, Feng Jianxing.A NEW INSTRUMENTAL METHOD FOR TOXICITY MONITORING[J].Acta Scientiarum Naturalium University Nankaiensis,1998,31(1):100-104. |
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| Authors: | Wang Lishi Zhang Baogui Chen Xulong Feng Jianxing |
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| Abstract: | This work described a new instrumental approach for monitoring the toxicity of aquatic samples. This bioassay is using the inhibition of dehydrogenase enzyme activity of mixed bacterial culture as teat parameter. The dehydrogenase activity is indicated by methylene blue. The data presented compare the instrument EC50 data for pure compounds on Microtox EC50. In addition 13 samples were assayed simultaneously using respiratory frequency of fish testing procedures. The data show that a good correlation exists between the instrumental assay and conventional testing procedures. The instrument displays good potential as a rapid and reliable method for assessing the toxicity of water samples. |
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| Keywords: | bacteria dehydrogenase toxicity instrumentation |
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