基于PiggyBac转座系统的低溢漏诱导型Tet-On过表达体系的建立及应用

为避免诱导基因稳定表达的Tet-On诱导表达系统溢漏表达,实现简便且高效的外源基因稳定诱导表达, 本研究拟在Tet-On调控的转录水平基础上,将基于稳定配体Shield-1的不稳定结构域FK506结合蛋白引入目的基因的N端,从蛋白水平控制其本底表达水平.为验证该系统的效果,本研究以荧光蛋白TdTomato为报告基因,经流式分析结果证明优化后的体系较原体系的溢漏表达在蛋白水平上降低7倍左右.将该系统应用于基于小鼠胚胎干细胞的体外牙向分化模型,在诱导因子Dox和稳定配体Shield-1的协同作用下,诱导表达牙齿发育相关转录因子Hand2提高了牙向分化诱导的完成度.     

平面梁单元的温度应力

介绍了能量原理在结构矩阵分析方面的原理及应用。以虚位移原理为理论依据,采用MATLAB符号运算的方法,分析了平面梁单元在荷载及温度变化影响下的单元刚度矩阵。计算表明:该方法在形成和求解由平面梁单元组成的工程结构时,具有较大的速度优势及良好的程序可读性。     

Construction of ice nucleation active <Emphasis Type="Italic">Enterobacter cloacae</Emphasis> for control of insect pests

Ice nucleation active(INA)bacteria are the most potent heterogeneous ice nuclei in nature,which have become an important biological resource for diverse applications.Many researches have proved that INA bacteria can raise the supercooling points(SCPs)of insect pests,then reduce their cold hardiness.However,INA bacteria‘s inefficient colonization on the surface or in the guts of insects,and the high incidence of frost injury induced by their release hampered the application of INA bacteria in controlling insect pests in agricultural fields.In this study,we constructed a recombinant plasmid mob-Tn5-iceA with the ability of broad-host-range conjugal mobilization and integration of the ina gene of icaA into chromosomal DNA of many gram-negative bacteria by Tn5 transposition.In addition,Ent.cloacae strains stably carrying iceA and expressing high ice nucleation activity(INA),even in the absence of antibiotic pressure,were constructed through conjugal mobilization and Tn5 transposition.Ent,cloacae strains have benn reported to be able to efficently colonize in the guts of insects,but have weak plant epiphytic ability.Therefore,these transgenic Ent.Cloacae may be promising candidates for control of insect pests in agricultural fields.     

环型二元序列的赋权对换排序问题

研究了环型二元序列的赋权对换排序问题。定义一个长度为l的对换的费用是f(l)=lα,0≤α<1,对环型二元序列的赋权对换排序问题给出了一个O(logn)近似算法,其中n是环型二元序列的长度。     

The generalized partial transposition criterion for infinite-dimensional quantum systems

The positive partial transposition(PPT)criterion and the realignment criterion constitute two of the most important criteria for detecting entanglement.The generalized partial transposition(GPT)criterion which contains the PPT criterion and the realignment criterion as special cases,provides a necessary condition for a multipartite state to be separable.Here we extend the GPT criterion to the infinitedimensional multipartite(bipartite)case and show that it includes multipartite(bipartite)PPT criterion and multipartite(bipartite)realignment criterion as special cases as well.     

具有共轭复数重极点系统的一种状态转移矩阵

本文将求解无共轭复数重极点的模式系统（Ｍ，Ｂ，Ｃ）的状态转移矩阵推广到求解具有任意重共轭复数极点的一般情况，得出了用实数表示的求解公式和两个重要的拉氏变换式。为简便、迅速、准确，特别便于计算机求解这类状态方程创造了有利条件，具有明显的效果和普遍的实用价值。     

确定变质岩区褶皱形态的新方法

本文提出确定变质岩区褶雏形态的一种新方法——构造透镜体法。这种方法的理论依据就是构造置换。     

Subcutaneous transposition of the spleen enhances the survival rate following 90% hepatectomy in rats

Summary The effects of subcutaneous transposition of the spleen (STS) on the survival rate following 90% hepatectomy were investigated in rats. The survival rate was significantly higher in the STS group than in the non-STS group. Light microscopy enabled us to note that congestion in the terminal portal veins and sinusoids occurred either slightly earlier or to a higher degree in the non-STS group.     

《押沙龙，押沙龙!》的互文性解读

《押沙龙,押沙龙!》标志着福克纳后现代主义的创作转向,其特征主要体现在互文性的写作策略之上。文章拟以文本结构、人物选择和神话模式的易位为切入点对文本进行互文性解读,以求对文学传统与文本独特性之间的关系进行新的思考。