重组人酸性成纤维细胞生长因子促进大鼠烫伤愈合的研究

目的:研究重组人酸性成纤维细胞生长因子(rh-aFGF)促进大鼠烫伤创面愈合的作用。方法:采用大鼠背部深II度烫伤模型,将3种不同浓度的rh-aFGF溶液隔日一次滴注于创面,观察伤后不同时间的创面面积变化和病理组织学改变。结果:rh-aFGF可明显加速大鼠皮肤创伤的愈合,使创面面积明显缩小(P<0 05)。病理组织学检查发现,rh-aFGF组创面伤后7d成纤维细胞生长活跃、数量多,其毛细血管胚芽与成纤维细胞数量显著多于溶媒对照组;伤后14d,创面收缩与再上皮化明显,新生上皮向创面中心爬行较快。结论:外用rh-aFGF对大鼠深II度烫伤创面有明显的促愈合作用。     

传染性法氏囊病病毒在鸡胚细胞上的优化繁殖

研究了传染性法氏囊病病毒在鸡胚细胞上繁殖上的优化条件，结果表明，鸡胚细胞体外增殖培养后对ＩＢＤＶ的敏感性并不减弱，而且有提高。维持培养基中血清浓度的变化对病毒的繁殖影响不大。     

Morphological effects of serotonin and ketanserin on embryonic chick skin in vitro

Summary Cell and organotypical cultures are used to study the direct effect of serotonin and of ketanserin, a serotonin antagonist, on dermal and epidermal cells of embryonic chick skin. Ketanserin stimulates the increase in cell number and inhibits the differentiation, whereas serotonin stimulates differentiation and inhibits the increase in cell number.     

Insulin-like effect of dichloroacetic acid on hexose transport in Swiss 3T3 cells

Summary Hexose transport in Swiss 3T3 cells was increased by treatment with dichloroacetic acid as well as by treatment with insulin. Neither extra-nor intracellular Ca²⁺ was found to be involved in their stimulatory action. On the other hand, the removal of intracellular Mg²⁺ resulted in a loss of the stimulation. These results suggest that dichloroacetic acid stimulates the hexose transport in Mg²⁺-dependent manner, similar to that of insulin.This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, and the Ministry of Health and Welfare of Japan.     

灌注速率对成纤维细胞在三维支架中生长和胶原合成的影响

为了降低工程化组织体外构建过程中的传质限制,采用自行研制的灌注生物反应器系统,以人皮肤成纤维细胞为模型细胞,以PET(Poly(ethylene terephthalate))为三维支架,构建工程化真皮组织,考察灌注速率对成纤维细胞生长和胞外基质合成的影响。将人皮肤成纤维细胞灌注接种于PET支架,分别以0.02、0.2、0.8 cm/min的灌注速率培养18 d。结果表明,灌注培养能促进细胞增殖,提高细胞在3D支架中均匀分布;与静态培养和0.02 cm/min的灌注速率相比,在0.2 cm/min和0.8 cm/min的灌注速率下进行培养,提高了细胞的葡萄糖比消耗速率,刺激细胞合成和分泌更多的胶原基质,但增加了流失到培养液中胶原的比例,灌注培养是体外构建工程化真皮组织的有效方法。     

N/C对碳氮膜修饰的多壁碳纳米管细胞相容性的影响

为改善碳纳米管的细胞相容性,通过离子束辅助沉积方法在由化学气相沉淀法制备的多壁碳纳米管表面沉积纳米厚度的碳氮膜以进行表面修饰.通过观察L929小鼠肺部成纤维细胞、EAHY926人内皮细胞和兔血红细胞在修饰前后的碳纳米管表面的黏附和生长状态可知,沉积CNx膜的多壁碳纳米管的细胞相容性优于沉积前,表现出良好的生命活性和增殖状态,同时,较高的N/C比有利于细胞在沉积CNx膜碳纳米管表面的增殖和生长以及细胞形态的伸展.     

Rapid reversion of aging phenotypes by nicotinamide through possible modulation of histone acetylation

Aging appears to be an irreversible process. Here we report that nicotinamide (NAA) can induce rapid and reversible reversion of aging phenotypes in human diploid fibroblasts in terms of cell morphology and senescence-associated β-galactosidase activity. Although NAA seems to enhance the replicative potential of the cells, it has little effect on their growth rate and life span, suggesting that NAA action is rather separated from the cellular replicative system. The effects are unique to NAA: none of the NAA-related compounds examined (an NAD precursor/niacin, NAD analogs, and poly(ADP-ribose) polymerase inhibitors) exerted similar effects. Thus, NAD-related metabolism and poly(ADP-ribosyl)ation are unlikely related to the NAA action. On the other hand, histone acetyltransferase (HAT) activity was elevated in NAA-exposed cells, while in aged cells, HAT activity and histone H4 acetylation were lowered. Taken together, the results suggest that NAA may cause rejuvenation by restoring, at least in part, altered gene expression in aged cells through its activation of HAT. Received 27 August 2001; received after revision 15 October 2001; accepted 15 October 2001     

DiI荧光标记示踪兔皮肤成纤维细胞修复前交叉韧带损伤的研究

体内采用组织工程方法构建前交叉韧带种子细胞筛选研究,提供了一种理想的细胞标记的方法.体外分离培养兔皮肤成纤维细胞(SF),比较皮肤成纤维细胞使用荧光染料CM-DiI体外标记前后细胞的生长状态、增殖活性以及检测SF复合纤维生物蛋白胶植入前交叉韧带上12周后的荧光表达情况.结果表明CM-Dil标记SF效果良好,标记阳性率高于95%.而标记前后SF增殖活性无明显变化,荧光标记在体内实验12周后仍有表达.从而证实CM-DiI是筛选前交叉韧带组织工程种子细胞筛选研究中细胞标记、示踪的良好标记物.     

吉林双阳梅花鹿成纤维细胞生长因子10的克隆和基因分析

根据多个物种的成纤维细胞生长因子10的序列,设计了特异的引物,从吉林双阳梅花鹿子宫cDNA库中得到了梅花鹿成纤维细胞生长因子10,并应用多种数据库和软件对其进行了分析．     

Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer

Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, slw3 and slw6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after activation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig fibroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive.