氧帘快速割嘴在连铸板坯切割的应用

将氧帘快速切割的原理应用于连铸板坯切割生产中，着重研究连铸板坯快速切割割嘴的工艺过程及结构特点。该割嘴在实际生产应用中表明具有切割成本低、工效高、寿命长、使用方便、性能可靠、割缝较窄、切割断面质量好等优点，具有 很高的推广价值。     

Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG₁ antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

Fibronectin peptide DRVPHSRNSIT and fibronectin receptor peptide DLYYLMDL arrest gastrulation ofRana pipiens

Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frogRana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373–1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124–131 of 1 subunit of a variety of integrins including 51. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s).     

杂交玉米酯酶电泳指纹的研究

本文运用聚丙烯酰胺凝胶电泳技术,以杂交玉米种胚为材料,研究制作了八个杂交种及其亲本自交系原种的种胚酯酶电泳指纹,其具有稳定、清晰、特异性强等特点．用其中的丹玉１３（Ｍｏ１７Ｈｔ×Ｅ２８）杂交组合,比较了用电泳指纹法与田间种植鉴定法．经检验人为掺混种子批中母本自交粒比率中的准确度,结果表明：指纹法与实际掺混水平更为接近,比田间种植鉴定的成熟期鉴定结果还要准确     

珍稀濒危植物四合木体细胞胚胎发生的组织学观察

通过对四合木愈伤组织诱导和体细胞胚胎形成过程中组织学观察的研究,得出以下结果：１）茎尖、茎段、肉质叶和花蕾四种外植体中花蕾的愈伤组织诱导率最高,其中花蕾的肉质花托是四合木体细胞胚胎发生的良好外植体来源;２）基本２基为改良Ｍｓ固体培养基;ａ．附加０．１ｍｇ／Ｌ ６－ＢＡ和０．５ｍｇ／Ｌ,２,４－Ｄ可诱导产生大量的淡黄色疏松状愈伤组织;ｂ．愈伤组织在去除外源植物生长调节物质的条件下,经一年左右继代培养     

柑橘类植物染色体制片新技术

为获得清晰的染色体图像,对柑橘的体细胞染色体和花粉母细胞染色体制片方法进行了研究.开发了染色体制 片新技术:①体细胞染色体制片选取3～5mm长的幼叶,用体积分数为0.3%的纤维素酶和体积分数为0.2%的果胶 酶于37℃下进行2h酶解;②花粉母细胞的染色体制片则选取约开花3周前花蕾中的花药,酶解的适宜条件为3%纤 维素酶和2%果胶酶混合酶液于37℃下进行3h酶解.可得到背景干净、伸展分散良好、清晰可靠的染色体图像.     

烟草体细胞杂交再生植株的影响因素

利用体细胞杂交技术,可以克服传统的有性杂交遇到的诸多育种障碍,加速烟草品种选育进程.本文着重对影响烟草体细胞杂交成株的相关因素进行了论述,并讨论了体细胞杂交技术在烟草育种上的应用潜力.     

胚期接种IBD疫苗对雏鸡ND4倍剂量免疫接种效果之影响

为了研究用IBD疫苗进行鸡胚免疫的可行性，探讨了胚期接种IBD疫苗对雏鸡ND4倍剂量免疫接种效果的影响．将IBD中等毒力疫苗接种18日龄鸡胚，出壳后采用4倍剂量ND疫苗进行免疫，通过抗体水平、免疫细胞数量及功能和免疫保护力等的检测，结果表明：胚期接种IBD中等毒力疫苗对雏鸡4倍剂量免疫仍具有免疫抑制作用．     

植物体细胞胚胎发生的理论研究

把体细胞胚胎发生现象看作开展生物学理论研究的良好模型，介绍了运用细胞生物学和分子生物学的原理与技术进行研究的成果，特别是体细胞胚胎发生相关基因的研究进展。