HPLC法测定喜树果中喜树碱和羟基喜树碱的含量

测定喜树果中喜树碱和10-羟基喜树碱的含量,采用高效液相色谱法,色谱柱为DiamonsilC18(5μm,200mm×4.5mm),乙腈:水(25:75)为流动相,检测波长254nm,流速1.0mL·min-1。结果:喜树碱在(0.0644～32.2)μg·mL-1、羟基喜树碱在(0.25～25)μg·mL-1范围内线性关系良好。说明方法分离效果好、专属性强、分析速度快、灵敏度高,可作为喜树果药材含量测定的方法。     

喜树碱及其衍生物的研究进展

喜树碱及其衍生物是一类具有抗癌活性的生物碱 .综述了喜树碱的性质、作用机理、提取制备和临床应用等方面的研究进展 .     

Preparation,Characterization, and Antitumor Efficacy of,Camptothecine-Loaded Hydroxyapatite / Poly （lactic-co,glycolic acid ） Composite Nanofibers via Electrospinning

In the past decade, various medicated nanofibrous scaffolds have been developed as effective drug delivery systems for postsurglcal cancer treatment. In this study, hydroxyapatite nanoparticles （HANPs） were used as carriers to load an anticancer （CPT）, and the CPT-Ioaded HANPs （CPT @HANPs） was then incorporated into poly（ lactic-co.glycolic acid） （PLGA） nanofibers via electrospinning. Thus fabricated medicated nanofibrous mats （ PLGA /CPT @ HANPs） were characterized by field emission scanning electron microscope （FESEM）, transmission electron microscope （TEM）, attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR） and X-ray diffraction （XRD）. The release prof＇des of CPT from the medicated electrospun mats were obtained and their in vitro anticancer efficacy against HeLa cells was also evaluated. The results showed that the CPT was successfully loaded onto the surface of HANPs, and the prepared electrospun mats exhibited a homogeneous and continuous morphology. Furthermore, the loaded CPT exhibited a sustained release behavior from the nanoflbrous mats and the released CPT showed a long-term anticancer efficacy against HeLa cells. Therefore, the prepared medicated electrospun mats may be served as an effective drug delivery device for local antitumor treatment.