井巷多断面岩层热传递方式与深井热环境动态分析

确定井巷风流热量来源及关键地点温度变化、热增量与损失量,并以之为依据有针对性地开展深井气候调节是通风工程需要解决的关键问题之一.通过改进传统的傅里叶导热模型,建立了基于通风井巷实际断面形状的三维热传导模型,针对不同井巷断面形状确定了反映流速与流量状态的动量相关的计算边界条件,并结合时间参数揭示了岩层内部导热以及井巷岩壁与气流间的热交换规律,建立了湿壁面与气流间的热湿传导方程.在此基础上,构建起国内某大型深井金属矿山井下通风系统,获得了关键地点的温度、湿度、热量等参数的走势和变化特点,为矿山开展深井气候调节与控制奠定了基础.     

The PDZ2 domain of zonula occludens-1 and -2 is a phosphoinositide binding domain

Zonula occludens proteins (ZO) are postsynaptic density protein-95 discs large-zonula occludens (PDZ) domain-containing proteins that play a fundamental role in the assembly of tight junctions and establishment of cell polarity. Here, we show that the second PDZ domain of ZO-1 and ZO-2 binds phosphoinositides (PtdInsP) and we identified critical residues involved in the interaction. Furthermore, peptide and PtdInsP binding of ZO PDZ2 domains are mutually exclusive. Although lipid binding does not seem to be required for plasma membrane localisation of ZO-1, phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P ₂) binding to the PDZ2 domain of ZO-2 regulates ZO-2 recruitment to nuclear speckles. Knockdown of ZO-2 expression disrupts speckle morphology, indicating that ZO-2 might play an active role in formation and stabilisation of these subnuclear structures. This study shows for the first time that ZO isoforms bind PtdInsPs and offers an alternative regulatory mechanism for the formation and stabilisation of protein complexes in the nucleus.     

白背三七叶组织培养研究(英文）

以白背三七幼叶和带芽茎段为外植体,探讨不同激素配比对白背三七离体繁殖的影响,建立白背三七种苗再生繁殖体系.结果表明：当NAA超过1.0 mg/L时,愈伤化都非常好,出愈率达到100%,但是当NAA浓度过高时,愈伤组织长势不好,不利于器官的诱导分化.继代培养中,1.0 mg/L NAA＋1.0 mg/L 6-BA更有利于愈伤组织的进一步增长,达到完全愈伤化.愈伤组织的生长曲线基本上呈现迟滞期、对数增长期和稳定期.在不定芽诱导分化中,1.0 mg/L NAA＋1.5 mg/L 6-BA最有利于芽的生长分化,过高浓度的6-BA时,不定芽生长较弱,不利于根分化及移栽.在带芽茎段诱导丛生芽的过程中,加入适量的IBA和赤霉素更有利于丛生芽的生长.根分化的过程中,高浓度的NAA抑制根的生长与分化.     

Computer Simulation of Dynamic Interactions Between Vehicle and Long Span Box Girder Bridges

Moving vehicle loads, associated with roadway traffic can induce significant dynamic effects on the structural behaviours of bridges, especially for long-span bridges. The main objective of current research is to study traffic induced dynamic responses of long-span box-girder bridges. The finite element method has been employed in this study to obtain a three-dimensional mathematical model for the bridge system. For vehicle-bridge dynamic interaction analysis, the vehicle is modeled as a more realistic three-axle, six-wheel system, and the corresponding dynamic interaction equations have been derived. The bridge-vehicle interaction is affected by many factors. The current study has been focused on such factors as: vehicle speed, vehicle damping ratio, multiple traffic lanes, mass ratio of vehicle and bridge, and dynamic characteristics of bridge. Case studies have been conducted to investigate these factors by using several box girder bridge examples including Confederation Bridge, the longest box girder bridge in the world.