科技创业孵化载体专业化发展问题与对策研究

进入新发展阶段后,科技创新创业活动对孵化服务提出了更高的要求,科技创业载体需要加快提升专业化、精准化服务能力.文章基于2016-2019年全国科技创业孵化载体发展数据,全面分析专业化科技创业孵化载体建设发展现状,剖析了科技创业孵化载体在专业化建设方面存在的方向不清、动力不足、能力不够、模式不明、机制不畅等问题,从建立评价指标体系、明确建设主体、提升服务能力、加强资源开放和政策供给等方面提出对策建议.     

根的存在定理的推广及其应用

众所周知，在《数学分析》中会遇到连续函数的一个重要定理，即根的存在定理，此定理对方程根的存在性判别起着重要作用.将这方面已有的定理进行推广，并用例题说明其应用情况.     

北京市怀柔区下坊村农业旅游发展的剖析

宝山镇下坊村位于怀柔区北部山区,自然环境优越,拥有较为完善的基础设施,处于重要的农业旅游发展时期.但在发展过程中,存在旅游从业人员综合素质不高、现有的旅游产品较为单一、村落环境较凌乱等问题.为了促进该村农业旅游的持续发展,建议应积极充分挖掘本地文化资源,打造特色旅游品牌.加强从业人员培训,更新旅游经营理念.兼顾环境整治和保护.     

Platelet-derived growth factor receptor beta identifies mesenchymal stem cells with enhanced engraftment to tissue injury and pro-angiogenic property

Mesenchymal stem cells (MSCs) are heterogeneous likely consisting of subpopulations with various therapeutic potentials. Here we attempted to acquire a subset of MSCs with enhanced effect in wound healing. We found that human placental MSCs expressing platelet-derived growth factor (PDGF) receptor (PDGFR)-β exhibited greater proliferation rates and generated more colony-forming unit-fibroblast (CFU-F), compared to PDGFR-β^? MSCs. Notably, PDGFR-β⁺ MSCs expressed higher levels of pro-angiogenic factors such as Ang1, Ang2, VEGF, bFGF and PDGF. When 10⁶ GFP-expressing MSCs were topically applied into excisional wounds in mice, PDGFR-β⁺ MSCs actively incorporated into the wound tissue, resulting in enhanced engraftment (3.92 ± 0.31 × 10⁵ remained in wound by 7 days) and accelerated wound closure; meanwhile, PDGFR-β^? MSCs tended to remain on the top of the wound bed with significantly fewer cells (2.46 ± 0.26 × 10⁵) engrafted into the wound, suggesting enhanced chemotactic migration and engraftment of PDGFR-β⁺ MSCs into the wound. Real-Time PCR and immunostain analyses revealed that the expression of PDGF-B was upregulated after wounding; transwell migration assay showed that PDGFR-β⁺ MSCs migrated eightfold more than PDGFR-β^? MSCs toward PDGF-BB. Intriguingly, PDGFR-β⁺ MSC-treated wounds showed significantly enhanced angiogenesis compared to PDGFR-β^? MSC- or vehicle-treated wounds. Thus, our results indicate that PDGFR-β identifies a subset of MSCs with enhanced chemotactic migration to wound injury and effect in promoting angiogenesis and wound healing, implying a greater therapeutic potential for certain diseases.     

Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating

Cilia beating is powered by the inner and outer dynein arms (IDAs and ODAs). These multi-subunit macrocomplexes are arranged in two rows on each outer doublet along the entire cilium length, except its distal end. To generate cilia beating, the activity of ODAs and IDAs must be strictly regulated locally by interactions with the dynein arm-associated structures within each ciliary unit and coordinated globally in time and space between doublets and along the axoneme. Here, we provide evidence of a novel ciliary complex composed of two conserved WD-repeat proteins, Fap43p and Fap44p. This complex is adjacent to another WD-repeat protein, Fap57p, and most likely the two-headed inner dynein arm, IDA I1. Loss of either protein results in altered waveform, beat stroke and reduced swimming speed. The ciliary localization of Fap43p and Fap44p is interdependent in the ciliate Tetrahymena thermophila.     

Preparation and electrochemical performance of double perovskite La_2CoMnO_6 nanofibers

Through electrospinning,La_2Co Mn O_6 nanofibers were prepared from a polyvinylpyrrolidone/lanthanum nitrate–cobalt acetate–manganese acetate(PVP/LCM)precursor and were used as electrode materials.The morphologies and structures of the samples were characterized by field-emission scanning electron microscopy(FE-SEM),transmission electron microscopy(TEM),X-ray diffraction(XRD),and Brunauer-Emmett-Teller(BET)specific surface area analysis.The results show that the prepared La_2Co Mn O_6 nanofibers are stable,one-dimensional structures formed from interconnected La_2Co Mn O_(6 )nanoparticles with a diamond-like crystal structure.The specific surface area of the fibers is 79.407 m~2·g~(-1).Electrochemical performance tests with a three-electrode system reveal the specific capacitance of the La_2Co Mn O_6 nanofibers as 109.7 F·g~(-1) at a current density of 0.5 A·g~(-1).After 1000 charge-discharge cycles at a current density of 1 A·g~(-1),the specific capacitance maintains 90.9%of its initial value,demonstrating a promising performance of the constraint capacitance and good cyclic stability.     

装配式地铁车站侧墙-顶板节点抗震性能试验研究

针对装配式地铁车站结构,提出了一种新型的车站顶板与侧墙连接节点.该节点通过车站侧墙顶部伸出的竖向U形钢筋与预制顶板两端伸出的水平U形钢筋搭接连接,从而实现侧墙与顶板间的内力传递.结合实际工程,设计制作了3个U形筋搭接构造形式各不相同的装配式足尺节点试件.通过拟静力试验,研究了节点试件的破坏形态、滞回性能、承载能力、变形性能以及耗能能力.结果表明,装配式节点试件的破坏形态包括U形筋弯弧内侧混凝土的局部受压破坏和侧墙剪切破坏.节点试件的位移延性系数为2.7-3.4,试件顶板端部的极限转角为1/22～1/27.增加节点中受拉钢筋的搭接长度,可以有效确保U形筋搭接连接在往复荷载作用下的有效性.     

SDN环境下基于KNN的DDoS攻击检测方法

DDo S攻击是当前互联网面临的主要威胁之一,如何快速准确地检测DDo S攻击是网络安全领域研究的热点问题。文中提出了一种在SDN环境下基于KNN算法的模块化DDo S攻击检测方法,该方法选取SDN网络的5个关键流量特征,采用优化的KNN算法对选取的流量特征进行流量异常检测,最后基于NOX控制器和Net FPGA交换机进行了实验验证。实验结果表明:相对其他的分类检测算法,所提的检测方案具有更高的识别率和更低的误报率。