Holocene palynological records and their responses to the controversies of climate system in the Shiyang River drainage basin

The Shiyang River drainage basin is located in the northwest margin of the Asian monsoon region. Previous studies reached different conclusions about Holocene climatic changes in the basin. Some studies suggested the Holocene climatic changes were mainly controlled by the Asian monsoon and that the climate was relatively humid during the early Holocene (11.6-7.1 cal ka BP). Other studies found the mid-Holocene Climatic Optimum (7.0-5.0 cal ka BP), and this climate condition was similar to the Holocene westerly wind pattern in arid Central Asia. The modern climate is affected by the Asian monsoon and westerly wind in the drainage basin, and the Holocene climatic records showed two different Holocene climatic patterns-a westerly wind pattern and monsoonal pattern. However, it remains unclear what caused the two different Holocene climatic patterns to co-exist in the region. The palynological records are the main evidence for the Holocene climatic changes in the basin. This paper focuses on palynological records for different parts of the drainage basin. Among them, QTH02, QTL-03 and Sanjiaocheng records are located in the terminal lake, and the Hongshuihe record is located in the middle reaches of the basin. In the terminal lake, the palynological records of QTH02 and QTL-03 are similar, but the Sanjiaocheng record differs. The difference is mainly affected by the variable pollen assemblages in the different locations of the lake basin. From comparison and synthesis of the four palynological records, we concluded that the millennial-scale Holocene climatic changes were affected by the combined effects of the Asian monsoon and westerly wind in the drainage basin, which show the complicated Holocene climatic pattern in the northwest margin of the Asian monsoon.     

Genetic analysis and fine mapping of the pubescence gene GL6 in rice (Oryza sativa L.)

The pubescence of the leaf blade surface is an important agronomic characteristic for rice morphology and significantly influences rice growth as well as physiological characteristics. This characteristic was analyzed in F1 and F2 plants derived by crossing cultivar 75-1-127 with the indica cultivar Minghui 63, as well as the glabrous cultivar Lemont and indica cultivar 9311. Results indicated that the pubescence of the leaf blade surface was a dominant trait and controlled by a single gene. The GL6 gene was primarily mapped on rice chromosome 6 with recessive F2 population derived from 75-1-127/Minghui 63 by combining bulked segregation analysis and recessive class analysis using the Mapmaker3.0/MapDraw software. The genetic distances between the simple sequence repeat markers RM20491 and RM20547 were 7.2 and 2.2 cM, respectively. The GL6 gene was fine mapped in the interval between InDel-106 and InDel-115 at genetic distances of 0.3 and 0.1 cM, respectively. The large, recessive F2 population was derived from 75-1-127/Minghui 63. A high-resolution genetic and physical map of GL6 was constructed. Derived from the map-based sequences published by the International Rice Genome Sequencing Project, the GL6 gene was localized at an interval of 79 (japonica) and 116.82 kb (9311) bracketed by InDel-106 and InDel-115 within the BAC accession numbers AP008403 and AP005760. Seven annotated genes (japonica) and eight annotated genes (9311) were present. The basis was further set for GL6 cloning and function analysis.