万向接轴损伤的灰色识别与预测分析

以反映结构损伤程度的固有频率作为损伤识别的特征参数,应用可以同时反映多种特征参数影响的灰色GM(1,n)模型对万向接轴的损伤问题进行了识别和预测分析。为提高预测精度和准确性,根据结构的固有频率随损伤程度的变化规律,采用了对原始数据进行归一化和二次累加处理的方法。通过识别实例表明,采用此方法对工程结构进行损伤特征识别分析能够取得较好的效果,为结构损伤识别和预测提供了新方法。     

Human growth hormone in urine: development of an ultrasensitive radiometric assay

An immunoradiometric assay for human growth hormone (HGH) has been developed which has a detection limit of 1 ng/l and can measure HGH in unextracted urine from normal children and adults. The assay is based on a two-step procedure, using a solid-phase goat-anti-HGH immunosorbent for immunoextraction and [125I]-labeled monoclonal HGH-antibody for detection and quantification. The assay is not affected by urea, NaCl or changes of pH from 5-8. The mean urine HGH concentration in normal children is 6.78 +/- 7.6 (SD) pg/ml, in patients with HGH-deficiency 1.3 +/- 0.9 pg/ml which increases to 11.7 +/- 13.4 pg/ml on the day of growth hormone injection.     

Generation and annotation of the DNA sequences of human chromosomes 2 and 4

Human chromosome 2 is unique to the human lineage in being the product of a head-to-head fusion of two intermediate-sized ancestral chromosomes. Chromosome 4 has received attention primarily related to the search for the Huntington's disease gene, but also for genes associated with Wolf-Hirschhorn syndrome, polycystic kidney disease and a form of muscular dystrophy. Here we present approximately 237 million base pairs of sequence for chromosome 2, and 186 million base pairs for chromosome 4, representing more than 99.6% of their euchromatic sequences. Our initial analyses have identified 1,346 protein-coding genes and 1,239 pseudogenes on chromosome 2, and 796 protein-coding genes and 778 pseudogenes on chromosome 4. Extensive analyses confirm the underlying construction of the sequence, and expand our understanding of the structure and evolution of mammalian chromosomes, including gene deserts, segmental duplications and highly variant regions.     

Accessory cell-dependent selection of specific T-cell functions

Activation of many T-cell functions depends on their interaction with antigen-presenting accessory cells which express I region associated (Ia) products. Cells expressing accessory cell function include those of the monocyte/macrophage lineage and dendritic cells. More recently, B cells and a number of tumour cell lines of macrophage or B-cell origin were shown to act as accessory cells in certain assays. We showed previously that normal peritoneal exudate macrophages (PEC) induced both T-cell proliferation as well as T-cell help, whereas various Ia+ tumour lines of macrophage or B-cell origin, although stimulating antigen-specific T-cell proliferation, did not significantly activate T-cell help. We report here that during the initial T-cell activation in vitro accessory cells (PEC or Ia+ tumour cells) select particular T cells to express previously determined functions. Moreover, some tumour cell lines induce suppressor T cells which inhibit helper activity.     

Separation of two components of adenovirus type 12 induced T-antigens with sephadex G-50

Zusammenfassung Es wird aus Hamstertumoren, die durch Adenovirus Typ 12 induziert wurden, ein Tumorantigenextrakt hergestellt. Dieser kann mit Sephadex G-50 Gelfiltration in 2 mittels Komplementbindung nachweisbare T-Antigen-Komponenten aufgetrennt werden.     

Hormonale Beeinflussung der menschlichen Tubenmotilität

Résumé Les expériences faites in vivo et in vitro démontrent la diminution de la sensibilité des trompes à l'égard de l'acétylcholine sous l'effet de la progestérone, ce qui a pour conséquence une réduction de leur motilité spontanée durant la phase de sécrétion. Par contre la sensibilité à l'adrénaline et à la sérotonine n'est pas modifiée au cours du cycle.

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Vortrag an der 37. Versammlung der Deutschen Gesellschaft für Gynäkologie, Travemünde 24. bis 28. September 1968.     

Stereocontrolled organocatalytic synthesis of prostaglandin PGF2α in seven steps

Prostaglandins are hormone-like chemical messengers that regulate a broad range of physiological activities, including blood circulation, digestion and reproduction. Their biological activities and their complex molecular architectures have made prostaglandins popular targets for synthetic organic chemists for over 40 years. Prostaglandin analogues are widely used as pharmaceuticals and some, such as latanoprost, which is used to treat glaucoma, have become billion-dollar drugs. Previously reported syntheses of these compounds are quite lengthy, and every chemical step costs time and energy, generates waste and is accompanied by material losses. Using a new bond disconnection, here we report a concise synthesis of the most complex prostaglandin, PGF2α, with high levels of control of relative and absolute stereochemistry, and fewer steps. The key step is an aldol cascade reaction of succinaldehyde using proline organocatalysis to create a bicyclic enal in one step and an enantiomeric excess of 98%. This intermediate bicyclic enal is fully primed with the appropriate functionality for attachment of the remaining groups. Access to this bicyclic enal will not only render existing prostaglandin-based drugs more affordable, but will also facilitate the rapid exploration of related chemical structures around the ubiquitous five-membered ring motif, such as potentially therapeutic prostaglandin analogues.