成骨细胞钙响应在稳恒、脉冲流体中的实时检测和研究

We investigated the intercellular calcium ([Ca²⁺]_i) responses in rat osteoblasts resulted fromboth steady and pulsed fluidic shear stress (F_ss) based on our design microfluidic device, by altering F_ssintensity, F_ss stimulation time (width) and interval time. Results showed that steady F_ss obviously induced[Ca²⁺]_i responses with two distinct phases: a peak phase and a plateau phase. The peak phase was sensitiveto the F_ss intensity, while plateau phase was dependent on application of F_ss width. Meanwhile, a reductivetendency of [Ca²⁺]_i responses was observed as experienced by different repetitive pulsed F_ss . This reductivetendency of [Ca²⁺]_i was alleviated with increment of interval time.