Detection of Vibrio anguillarum and Its Virulent Metalloprotease Using the Method of ELISA

A method detecting pathogenic Vibrio anguillarum and its virulent metalloprotease is reported in this paper. The metalloprotease is isolated from extracellular product (ECP) of V.anguillarum by the cellophane plate technique and purified by gel filtration and ion-exchange chromatography. Anti-sera are prepared by injecting V.anguillarum cells and metalloprotease into the rabbits. Slide Agglutination Assay is used to detect V.anguillarum in the infection experiment and Enzyme Linked Immunosorbent Assay (ELISA) is carried out to detect concentration of metalloprotease. The result shows that bacterium strain M3 is able to diffuse into the viscera of infected fish through the blood circulating system 10 hours after intramuscular infection, and ELASA is a sensitive method to detect the metalloprotease with detectable amount of 7.8ng. The aim of this study is to establish a sensitive and specific method to observe the infection of V.anguillarum in the host.     

典型海洋生态系统中关键种的研究进展

随着人类对生态环境和生物多样性保护意识的增强，基于食物网结构和物种间相互作用的关键种(Keystone species)这一概念一经提出就引起了广泛关注和讨论，相关研究亦成为国际生态学研究的热点。本文从关键种概念起源出发，探讨了关键种定义中丰度与作用的不成比例性、在实际应用中存在的模糊性和无法量化等问题，综述了不同类型海洋生态系统中关键种的特点和共性问题，探讨人类作为超级关键种(Hyperkeystone species)对海洋的影响，并尝试提出人类应当如何理性看待关键种及其在海洋生态系统中的功能和作用。