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Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter 总被引:81,自引:0,他引:81
Donovan A Brownlie A Zhou Y Shepard J Pratt SJ Moynihan J Paw BH Drejer A Barut B Zapata A Law TC Brugnara C Lux SE Pinkus GS Pinkus JL Kingsley PD Palis J Fleming MD Andrews NC Zon LI 《Nature》2000,403(6771):776-781
Defects in iron absorption and utilization lead to iron deficiency and overload disorders. Adult mammals absorb iron through the duodenum, whereas embryos obtain iron through placental transport. Iron uptake from the intestinal lumen through the apical surface of polarized duodenal enterocytes is mediated by the divalent metal transporter, DMTi. A second transporter has been postulated to export iron across the basolateral surface to the circulation. Here we have used positional cloning to identify the gene responsible for the hypochromic anaemia of the zebrafish mutant weissherbst. The gene, ferroportin1, encodes a multiple-transmembrane domain protein, expressed in the yolk sac, that is a candidate for the elusive iron exporter. Zebrafish ferroportin1 is required for the transport of iron from maternally derived yolk stores to the circulation and functions as an iron exporter when expressed in Xenopus oocytes. Human Ferroportin1 is found at the basal surface of placental syncytiotrophoblasts, suggesting that it also transports iron from mother to embryo. Mammalian Ferroportin1 is expressed at the basolateral surface of duodenal enterocytes and could export cellular iron into the circulation. We propose that Ferroportin1 function may be perturbed in mammalian disorders of iron deficiency or overload. 相似文献
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Cell-specific mitotic defect and dyserythropoiesis associated with erythroid band 3 deficiency 总被引:11,自引:0,他引:11
Paw BH Davidson AJ Zhou Y Li R Pratt SJ Lee C Trede NS Brownlie A Donovan A Liao EC Ziai JM Drejer AH Guo W Kim CH Gwynn B Peters LL Chernova MN Alper SL Zapata A Wickramasinghe SN Lee MJ Lux SE Fritz A Postlethwait JH Zon LI 《Nature genetics》2003,34(1):59-64
Most eukaryotic cell types use a common program to regulate the process of cell division. During mitosis, successful partitioning of the genetic material depends on spatially coordinated chromosome movement and cell cleavage. Here we characterize a zebrafish mutant, retsina (ret), that exhibits an erythroid-specific defect in cell division with marked dyserythropoiesis similar to human congenital dyserythropoietic anemia. Erythroblasts from ret fish show binuclearity and undergo apoptosis due to a failure in the completion of chromosome segregation and cytokinesis. Through positional cloning, we show that the ret mutation is in a gene (slc4a1) encoding the anion exchanger 1 (also called band 3 and AE1), an erythroid-specific cytoskeletal protein. We further show an association between deficiency in Slc4a1 and mitotic defects in the mouse. Rescue experiments in ret zebrafish embryos expressing transgenic slc4a1 with a variety of mutations show that the requirement for band 3 in normal erythroid mitosis is mediated through its protein 4.1R-binding domains. Our report establishes an evolutionarily conserved role for band 3 in erythroid-specific cell division and illustrates the concept of cell-specific adaptation for mitosis. 相似文献
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