萨满医术:北方民族精神医学

治病是北方少数民族萨满的主要职能之一。在信仰萨满教的民族中萨满往往充当民间医生的角色。萨满医术是一种精神医术和心灵医术。萨满主要是治心因性的精神疾病,其主要治疗手段也是一种心理治疗。在萨满医术中包含着现代精神医学中使用的一些治疗方法和治愈机制。萨满的精神医术之本质在于通过各种方式使患者的心理得到平衡,与此同时让患者振作起来,对自己的病情持乐观的态度,确立战胜病魔的信心。萨满精神医术就是一种典型的宗教性心理——生理调控术。对萨满医术,应从心理人类学、医学人类学、宗教人类学等多种角度进行研究,这样才能够得出比较全面而相对正确的结论。     

The molecular sociology of the cell

Proteomic studies have yielded detailed lists of the proteins present in a cell. Comparatively little is known, however, about how these proteins interact and are spatially arranged within the 'functional modules' of the cell: that is, the 'molecular sociology' of the cell. This gap is now being bridged by using emerging experimental techniques, such as mass spectrometry of complexes and single-particle cryo-electron microscopy, to complement traditional biochemical and biophysical methods. With the development of integrative computational methods to exploit the data obtained, such hybrid approaches will uncover the molecular architectures, and perhaps even atomic models, of many protein complexes. With these structures in hand, researchers will be poised to use cryo-electron tomography to view protein complexes in action within cells, providing unprecedented insights into protein-interaction networks.     

Zum Vorkommen neuraminsäurehaltiger Glykoproteide

Summary The results are reported of investigations on the content of neuraminic acid in serum of 15 animal species, pure serum protein fractions, fibrin, haemoglobin, urine, liquor, saliva and milk.     

安哥拉山羊及其杂种羊DNA指纹图谱分析

应用寡苷酸探针(CA/GATA/TCC)     

The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum

Thermoplasma acidophilum is a thermoacidophilic archaeon that thrives at 59 degrees C and pH 2, which was isolated from self-heating coal refuse piles and solfatara fields. Species of the genus Thermoplasma do not possess a rigid cell wall, but are only delimited by a plasma membrane. Many macromolecular assemblies from Thermoplasma, primarily proteases and chaperones, have been pivotal in elucidating the structure and function of their more complex eukaryotic homologues. Our interest in protein folding and degradation led us to seek a more complete representation of the proteins involved in these pathways by determining the genome sequence of the organism. Here we have sequenced the 1,564,905-base-pair genome in just 7,855 sequencing reactions by using a new strategy. The 1,509 open reading frames identify Thermoplasma as a typical euryarchaeon with a substantial complement of bacteria-related genes; however, evidence indicates that there has been much lateral gene transfer between Thermoplasma and Sulfolobus solfataricus, a phylogenetically distant crenarchaeon inhabiting the same environment. At least 252 open reading frames, including a complete protein degradation pathway and various transport proteins, resemble Sulfolobus proteins most closely.     

安哥拉山羊及其杂种羊父权认定的DNA指纹图分析

应用ＤＮＡ指纹技术对安哥拉山羊作亲子鉴定,结果发现子代个体的ＤＮＡ指纹图中,谱带均分别来自父亲和母亲,没有新带出现。父权概率为０．９８８９,表明（ＣＡ／ＧＡＴＡ／ＴＣＣ）５探针能有效地应用于安哥拉山羊及其杂种后代的遗传分析。     

Complete nucleotide sequence of the AIDS virus, HTLV-III

The complete nucleotide sequence of two human T-cell leukaemia type III (HTLV-III) proviral DNAs each have four long open reading frames, the first two corresponding to the gag and pol genes. The fourth open reading frame encodes two functional polypeptides, a large precursor of the major envelope glycoprotein and a smaller protein derived from the 3'-terminus long open reading frame analogous to the long open reading frame (lor) product of HTLV-I and -II.     

Snapshots of nuclear pore complexes in action captured by cryo-electron tomography

Nuclear pore complexes reside in the nuclear envelope of eukaryotic cells and mediate the nucleocytoplasmic exchange of macromolecules. Traffic is regulated by mobile transport receptors that target their cargo to the central translocation channel, where phenylalanine-glycine-rich repeats serve as binding sites. The structural analysis of the nuclear pore is a formidable challenge given its size, its location in a membranous environment and its dynamic nature. Here we have used cryo-electron tomography to study the structure of nuclear pore complexes in their functional environment, that is, in intact nuclei of Dictyostelium discoideum. A new image-processing strategy compensating for deviations of the asymmetric units (protomers) from a perfect eight-fold symmetry enabled us to refine the structure and to identify new features. Furthermore, the superposition of a large number of tomograms taken in the presence of cargo, which was rendered visible by gold nanoparticles, has yielded a map outlining the trajectories of import cargo. Finally, we have performed single-molecule Monte Carlo simulations of nuclear import to interpret the experimentally observed cargo distribution in the light of existing models for nuclear import.     

A Kind of Systems Methodology——The SPIPRO Principle

This paper deals with the Self-Increasing-Difficulty system (SIDIS) separated from general systems, with emphasis on the relevant methodology based on the Spiral Combining Propulsion Principle (SPIPRO principle in short). The system feature and its causative factors are analysed, and the implications and mechanism of the SPIPRO principle are discussed. Finally, the application possibilities of this methodology are illustrated with some examples.