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Xue QG Itoh N Schey KL Li YL Cooper RK La Peyre JF 《Cellular and molecular life sciences : CMLS》2007,64(1):82-95
A new lysozyme (cv-lysozyme 2) with a MALDI molecular mass of 12 984.6 Da was purified from crystalline styles and digestive
glands of eastern oysters (Crassostrea virginica) and its cDNA sequenced. Quantitative real time RT-PCR detected cv-lysozyme 2 gene expression primarily in digestive gland
tissues, and in situ hybridization located cv-lysozyme 2 gene expression in basophil cells of digestive tubules. Cv-lysozyme 2 showed high amino
acid sequence similarity to other bivalve mollusk lysozymes, including cv-lysozyme 1, a lysozyme recently purified from C. virginica plasma. Differences between cv-lysozyme 2 and cv-lysozyme 1 molecular characteristics, enzymatic properties, antibacterial
activities, distribution in the oyster body and site of gene expression indicate that the main role of cv-lysozyme 2 is in
digestion. While showing that a bivalve mollusk employs different lysozymes for different functions, findings in this study
suggest adaptive evolution of i type lysozymes for nutrition.
Received 30 August 2006; received after revision 14 October 2006; accepted 6 November 2006 相似文献
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