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1.
Christian Dölle Marc Niere Emilia Lohndal Mathias Ziegler 《Cellular and molecular life sciences : CMLS》2010,67(3):433-443
Poly-ADP-ribose polymerases (PARPs) use NAD+ as substrate to generate polymers of ADP-ribose. We targeted the catalytic domain of human PARP1 as molecular NAD+ detector into cellular organelles. Immunochemical detection of polymers demonstrated distinct subcellular NAD+ pools in mitochondria, peroxisomes and, surprisingly, in the endoplasmic reticulum and the Golgi complex. Polymers did not
accumulate within the mitochondrial intermembrane space or the cytosol. We demonstrate the suitability of this compartment-specific
NAD+ and poly-ADP-ribose turnover to establish intra-organellar protein localization. For overexpressed proteins, genetically
endowed with PARP activity, detection of polymers indicates segregation from the cytosol and consequently intra-organellar
residence. In mitochondria, polymer build-up reveals matrix localization of the PARP fusion protein. Compared to presently
used fusion tags for subcellular protein localization, these are substantial improvements in resolution. We thus established
a novel molecular tool applicable for studies of subcellular NAD metabolism and protein localization. 相似文献
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Summary Bovine insulin, proinsulin, and intermediate form were prepared by Sephadex gelfiltration and chromatography on DEAE-cellulose. Using a goat anti-insulin serum, the cross-reactions of proinsulin and intermediate form could be shown by immuno-precipitation. A method is described for the separation of proinsulin from insulin in agar gel. New compounds related to proinsulin or insulin were found in human insulin preparations by means of immunoelectrophoresis.
Wir danken Frl.B. Laars und HerrnD. Klatt für ausgezeichnete technische Mitarbeit. 相似文献
Wir danken Frl.B. Laars und HerrnD. Klatt für ausgezeichnete technische Mitarbeit. 相似文献
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TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function 总被引:2,自引:0,他引:2
Zhou L Lopes JE Chong MM Ivanov II Min R Victora GD Shen Y Du J Rubtsov YP Rudensky AY Ziegler SF Littman DR 《Nature》2008,453(7192):236-240
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Summary Adrenaline (A) is synthesized in the adrenal medullae by the enzyme phenylethanolamine-N-methyltransferase (PNMT). After surgical removal of the adrenal medullae tissue A levels ranged from 22% of control in the heart to 125% of control in the liver. Use of a novel assay to measure tissue A formation revealed that many tissues can synthesize A using PNMT and another enzyme that N-methylates both noradrenaline and dopamine. These enzymes are non-neuronal, inducible and synthesize a major fraction of tissue and urine A. 相似文献
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Brigitte Ziegler Barbara Wilke M. Ziegler S. Schmidt H. Zühlke 《Cellular and molecular life sciences : CMLS》1980,36(4):467-468
Summary Isolated islets from C57B1/6J mice exposed to 10 mmoles/1 glucose supplemented with 5 g/ml glibenclamide for 48 h released significantly less insulin in the subsequent short-time incubation thanuntreated controls (without glibenclamide), whereas insulin biosynthesis was not suppressed by glibenclamide treatment.Investigations were carried out as a part of the ForschungsprojektDiabetes mellitus und Fettstoffwechselstörungen, supported by the Ministry of Health of the GDR. 相似文献
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