Immunpräzipitation Insulin-verwandter proteine mit ziegen-anti-insulinserum |
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| Authors: | M Ziegler K -D Kohnert U Karg |
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| Institution: | (1) Zentralinstitut für Diabetes « Gerhardt Katsch », Experimenteller Bereich, 2201 Karlsburg, DDR |
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| Abstract: | Summary Bovine insulin, proinsulin, and intermediate form were prepared by Sephadex gelfiltration and chromatography on DEAE-cellulose. Using a goat anti-insulin serum, the cross-reactions of proinsulin and intermediate form could be shown by immuno-precipitation. A method is described for the separation of proinsulin from insulin in agar gel. New compounds related to proinsulin or insulin were found in human insulin preparations by means of immunoelectrophoresis.
Wir danken Frl.B. Laars und HerrnD. Klatt für ausgezeichnete technische Mitarbeit. |
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