Position of the Y-chromosome at somatic metaphase in patients with chronic myelogenous leukemia (CML)

Summary A random distribution of the Y-chromosome at somatic metaphase was found in 50 patients with Ph' positive chronic myelogenous leukemia (CML). Thus, it is concluded that the positive of the Y-chromosome at somatic metaphase does not appear to influence the loss from bone marrow cells.     

Studies on induction hardening of powder-metallurgy-processed Fe-Cr/Mo alloys

Induction hardening of dense Fe-Cr/Mo alloys processed via the powder-metallurgy route was studied. The Fe-3Cr-0.5Mo, Fe-1.5Cr-0.2Mo, and Fe-0.85Mo pre-alloyed powders were mixed with 0.4wt%, 0.6wt%, and 0.8wt% C and compacted at 500, 600, and 700 MPa, respectively. The compacts were sintered at 1473 K for 1 h and then cooled at 6 K/min. Ferrite with pearlite was mostly observed in the sintered alloys with 0.4wt% C, whereas a carbide network was also present in the alloys with 0.8wt% C. Graphite at prior particle boundaries led to deterioration of the mechanical properties of alloys with 0.8wt% C, whereas no significant induction hardening was achieved in alloys with 0.4wt% C. Among the investigated samples, alloys with 0.6wt% C exhibited the highest strength and ductility and were found to be suitable for induction hardening. The hardening was carried out at a frequency of 2.0 kHz for 2-3 s. A case depth of 2.5 mm was achieved while maintaining the bulk (interior) hardness of approximately HV 230. A martensitic structure was observed on the outer periphery of the samples. The hardness varied from HV 600 to HV 375 from the sample surface to the interior of the case hardened region. The best combination of properties and hardening depth was achieved in case of the Fe-1.5Cr-0.2Mo alloy with 0.6wt% C.     

A rapid colorimetric method for the determination of tobacco mosaic virus concentration in plant saps

Zusammenfassung Es wird eine neue kolorimetrische Schnellmethode zur Bestimmung des Nucleoproteidgehaltes von Tabakmosaikvirus im Saft von Tabakblättern beschrieben. Der Saft wird durch die Chloroform-Wasser-Emulsionstechnik geklärt, das Virus-Nucleoproteid durch 2,0M Trichloressigsäure gefällt und dann in 1,0N NaOH gelöst. Die durch Zusatz von Folin-Phenolreagens entstandene Farbe wird kolorimetrisch bestimmt. Die mit der neuen Methode erhaltenen Resultate stimmen gut mit denjenigen von Aktivvirusbestimmungen überein.     

The knockout mouse project

Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.     

Cytopathological effects of aristolochic acid on male houseflies causing sterility

Summary Feeding male houseflies with 0.075% aristolochic acid obtained from the plants of the genusAristolochia induced sterility. High condensation of chromosomes, and breakages, have been shown to be responsible for sterility. When onion roots were exposed to aristolochic acid similar chromosomal aberrations were noticed in cells at the tip.Authors are thankful to Prof. Dr G. Rücker (Universität Bonn) for a generous gift of aristolochic acid.     

The distribution of nutrients in the seed parts of bengal gram

Zusammenfassung Die bengalische «Gram»-Pflanze (Cicer arietinum L.) ist in Samenschale, Cotyledonen und Restteile des Embryos zerlegt worden. In diesen Fragmenten wurde die Verteilung von Protein, Extraktstoffen, Asche, Faseranteilen sowie Calzium, Phosphor und Eisen näher untersucht. In den relativ massiven Samenschalen fand sich auffallend viel Calcium, während die übrigen untersuchten Stoffe in den andern Pflanzenteilen (mit Ausnahme vom Faseranteil) nur geringfügig vorhanden waren. Die Cotyledonen, die mehr als 85% dieses Getreides ausmachen, zeigen chemisch ausgeglichene Verhältnisse.     

The effect of methyltestosterone and 17β-estradiol on DNA synthesis of mesonephric blastemae of frog larvae

Résumé L'addition d'une concentration masculinisante de méthyl testostérone aux larves deRana pipiens stimule la synthèse de l'ADN dans leurs blastèmes mésonéphrétiques. On n'observe aucun effet semblable sur la synthèse de l'ADN quand on emploie la concentration masculinisante ou féminisante de l'estradiol.