矮壮素对非洲菊转基因苗生长的促进作用

转基因非洲菊幼苗经过多代抗生素筛选,生长受到很大的影响．为了更好地利用珍贵的转基因苗,采用了矮壮素结合其他生长调节因子来促进转基因苗的生长和生根的试验研究,发现添加0．2 mg／L矮壮素能大大提高转基因苗的生根能力和移栽成活率．     

Fine mapping of the red plant gene R_1 in upland cotton (Gossypium hirsutum)

Sub 16 is a substitution line with G. hirsutum cv. TM-1 genetic background except that the 16th chro-mosome (Chr. 16) is replaced by the corresponding homozygous chromosome of G. barbadense cv. 3-79, and T586 is a G. hirsutum multiple gene marker line with 8 dominant mutation genes. The R1 gene for anthocyanin pigmentation was tagged in Chr. 16 in T586. The objective of this research was to screen SSR markers tightly linked with R1 by using the F2 segregating population containing 1259 plants derived from t...     

不结球白菜SSR引物的高效开发及其通用性研究

利用改进的ISSR-PCR分离不结球白菜基因组微卫星,进行两步PCR,分别设计出SSR两端引物IP2和IP3（即SSR引物对）,SSR引物产率为12%,明显高于传统方法.不结球白菜SSR序列以（GA）n为主,占70%.将69对SSR引物用于芸苔属其它8种作物的扩增,97%引物有显著扩增,扩增率最高的为四倍体白菜和甘蓝（85.5%）,最低的为青花菜（49.3%）.10对为通用引物,在所检测的8种作物中片段大小一致,其余的扩增片段和数目差异较大,表现出较为丰富的多态性.尤其是在C基因组的甘蓝和花椰菜上,最多可检测到5个位点,表明运用lSSR-PCR开发出的引物多态性较高.