The absence of an inhibitory effect of metyrapone (2-methyl-1,2-di (3-pyridyl) propan-1-one) on hepatic microsomal hydroxylation in scurvy.

Microsomes from livers of scorbutic guinea-pigs showed a reduced rate of acetanilide hydroxylation. The response of "scorbutic" liver microsomes to the inhibitor Metyrapone (2-methyl-1,2 di (3-pyridyl) propan-1-one) was different from that of liver microsomes from non-scorbutic guinea-pigs.     

BP网络应用于黄河水质的预测研究

利用黄河兰州段水质指标浓度时间序列作为学习样本，选取了DO、COD、氨氮、重金属离子等l0项指标作为输出参数，运用人工神经网络BP模型中的Levenberg—Marguardt优化算法对学习样本优化建模，将优化好的网络的预测结果与实测结果进行了比较，表明BP网络可以很好地用于黄河水质指标值及水质类别的预测。     

基于改进的卷积神经网络的钢号识别

为获取样本的多样性特征,提出了一种改进的卷积神经网络结构。该网络中引入多层递归神经网络,利用卷积神经网络提取输入图像的浅层特征,同时利用卷积神经网络和递归神经网络并行提取高层特征,最后将两种网络学习到的特征进行融合输入到分类器中分类。利用迁移学习理论解决小样本集数据训练不足的问题,并将这种卷积神经网络结构应用于石油物资管线钢号识别中。实验探究了递归神经网络个数与卷积核个数对网络性能的影响,实验结果表明,改进的网络结构与其它网络进行对比,错误率降低了 3% 。     

Structural basis for cofactor-independent dioxygenation in vancomycin biosynthesis

Enzyme-catalysed oxidations are some of the most common transformations in primary and secondary metabolism. The vancomycin biosynthetic enzyme DpgC belongs to a small class of oxygenation enzymes that are not dependent on an accessory cofactor or metal ion. The detailed mechanism of cofactor-independent oxygenases has not been established. Here we report the first structure of an enzyme of this oxygenase class in complex with a bound substrate mimic. The use of a designed, synthetic substrate analogue allows unique insights into the chemistry of oxygen activation. The structure confirms the absence of cofactors, and electron density consistent with molecular oxygen is present adjacent to the site of oxidation on the substrate. Molecular oxygen is bound in a small hydrophobic pocket and the substrate provides the reducing power to activate oxygen for downstream chemical steps. Our results resolve the unique and complex chemistry of DpgC, a key enzyme in the biosynthetic pathway of an important class of antibiotics. Furthermore, mechanistic parallels exist between DpgC and cofactor-dependent flavoenzymes, providing information regarding the general mechanism of enzymatic oxygen activation.     

油田管道风险及完整性评价体系构建与应用

为提升油田管道安全隐患的预警和分析能力，根据 GB32167-2015《油气输送管道完整性管理规范》的要 求和管道密集型油田的实际特点，对Ⅲ类管道进一步精细划分; 同时结合地理和周边居住区情况，对管道的高 后果区进行有效识别，从而对管道进行风险等级分类，完善管道完整性的评价体系。通过互联网 + 数据驱动的 模式建立油田管道完整性数据管理平台，以可视化方式全面评价油田管道运行风险。该平台可提高油田管道 风险评估的准确性，确保及时准确预测风险。     

无人机航迹规划常用算法综述

为促进航迹规划技术的发展,对航迹规划常用算法进行综述。首先对航迹规划的规划思想和构成进行分析; 其次将航迹规划算法分为传统经典算法和现代智能算法两大类,对其中几种常用算法进行分析总结; 最后阐述现代智能算法在航迹规划应用中的改进、多重算法的融合改进以及多无人机四维航迹规划算法研究3 个研究热点及未来发展趋势。     

火灾报警系统的研究

介绍了火灾报警系统的硬件设置与软件设计原理，讨论了该系统安装调试过程中的有关技术问题，阐述了对该系统进行的模拟联动试验，证明该系统的技术是先进的，工作是可靠的，每个工作环节都准确无误。     

Cloning and sequencing of human cholesteryl ester transfer protein cDNA

The transfer of insoluble cholesteryl esters among lipoprotein particles is a vital step in normal cholesterol homeostasis and may be involved in the development of atherosclerosis. Extrahepatic tissues lack the enzymes required for the degradation of sterols to the excretable form of bile acids. Cholesterol synthesized in these tissues in excess of that needed for the synthesis of cell membranes or steroid hormones must accordingly be returned through the plasma to the liver for catabolism. The series of reactions involved has been termed reverse cholesterol transport. Catalysed steps of this pathway are believed to include an efflux from peripheral cells, which generates a diffusion gradient between these membranes and extracellular fluid; esterification of this cholesterol by lecithin-cholesterol acyltransferase (LCAT) (phosphatidylcholine-sterol acyltransferase) acting on species of high-density lipoproteins; transfer of the cholesteryl esters formed (largely to low- and very low-density lipoproteins) (LDL and VLDL) by a cholesteryl ester transfer protein (CETP); and removal of these lipoproteins, together with their cholesteryl ester content, by the liver through receptor-mediated and nonspecific endocytosis. Of these steps, the CETP reaction is the least characterized. Several laboratories have reported the purification from human plasma of proteins active on cholesteryl ester transfer between lipoprotein particles and possibly between cells and plasma. However, the reported relative molecular mass (Mr), abundance and specificity of the purified activities have differed considerably. We have recently described the preparation of a highly active CETP of Mr 74,000 purified about 100,000-fold from human plasma, which may represent the functional component of earlier preparations. Using a partial amino-acid sequence from this purified protein, CETP complementary DNA derived from human liver DNA has been cloned and sequenced and the cloned DNA used to detect CETP messenger RNA in a number of human tissues.     

Gemini表面活性剂在气/液界面上的微区形貌随表面压的变化

测定了Gemini表面活性剂在pH值为10．86,NaBr浓度为1．0mol／L的溶液气／液界面上的表面压一分子面积的等温线。用自制的Brewster角显微镜(BAM)观察了由Gemini表面活性剂在界面上所形成单分子膜的微区形貌随表面压的变化。结果表明：当pH值为10．86时,Gemini表面活性剂在1．0mol／L NaBr溶液的气／液界面上生成了凝聚态的单分子膜;当表面压较低时,观察到在界面上形成了同心圆结构;随着表面压的增加,这些同心圆可发生聚并而形成多中心结构。