LHβmRNA在文昌鱼脑和哈氏窝的定位及其表达

为确定存在干脑和哈氏窝的脊椎动物促进性腺激素样物质是由自身合成的还是有其他来源,用地高辛标记的黄体素β亚单位核糖核酸（LHβmRNA）探针对文昌鱼脑和哈氏窝进行原位杂交组织化学研究,结果发现LHβmRNA能够在文昌鱼脑的神经细胞和哈氏窝上皮细胞表达,这在分子水平上为哈氏窝与脊椎动物垂体同源以及为促性腺激素功能的演化提供了新证据。     

LHβmRNA在文昌鱼脑和哈氏窝的定位及其表达

为确定存在于脑和哈氏窝的脊椎动物促性腺激素样物质是由自身合成的还是有其他来源,用地高辛标记的促黄体素β亚单位核糖核酸(LH_βRNA)探针对文昌鱼脑和哈氏窝进行原位杂交组织化学研究.结果发现LH_βmRNA能够在文昌鱼脑的神经细胞和哈氏窝上皮细胞表达,这在分子水平上为哈氏窝与脊椎动物脑垂体同源以及为促性腺激素功能的演化提供了新证据.     

Localization and expression of LHβmRNA in the brain and Hatschek's pit of amphioxus, Branchiostoma belcheri

To determine whether gonadotropin-like substance in the brain and Hatschek' s pit of amphioxus issynthesized by the tissue in situ or transported from other tissue, a histochemical study was carried out by in situ hy- bridization using digoxigenin (DIG)-labelled LHβRNAprobes. The results showed that LHβmRNA expressed in the nerve cells of brain and the epithelial cells of Hatschek' s pit, thus providing new evidence for the homology of pituitary of ver- tebrates with Hatschek' s pit and the functional evolution of gonadotropin.