基于神经网络的企业信用等级评估

企业信用等级评估是金融领域重要的问题，论文采用人工神经网络模型研究企业信用等级的评估问题，按照企业样本在信用等级的分布状况来抽样，然后，根据企业样本性质的不同，将其分为制造业和非制造业两大类，利用偏相关分析方法建立了企业信用评级的指标体系，此外，还介绍了几种企业信用评级常用的评估模型，并将神经网络评估模型的性能和其他的信用评估模型作了比较，实验结果表明神经网络模型具有更好的预测准确性。     

管理系统的最经济控制问题

依据最经济控制问题的概念及其对无确定性数学模型系统分析存在的局限性，对现代管理系统最经济控制问题，提出了在满足系统基本要求前提下。实现控制功能及品质与控制成本比最佳或最划算的模型定义，并对管理系统的可靠性、ABC管理法、WSR方法论等方面的可靠性成本、运行成本和最佳收益等最经济控制问题进行了研究．     

Nras loss induces metastatic conversion of Rb1-deficient neuroendocrine thyroid tumor

Mutations in the gene encoding the retinoblastoma tumor suppressor predispose humans and mice to tumor development. Here we have assessed the effect of Nras loss on tumor development in Rb1 heterozygous mice. Loss of one or two Nras alleles is shown to significantly reduce the severity of pituitary tumors arising in Rb1(+/-) animals by enhancing their differentiation. By contrast, C-cell thyroid adenomas occurring in Rb1(+/-) mice progress to metastatic medullary carcinomas after loss of Nras. In Rb1(+/-)Nras(+/-) animals, distant medullary thyroid carcinoma metastases are associated with loss of the remaining wild-type Nras allele. Loss of Nras in Rb1-deficient C cells results in elevated Ras homolog family A (RhoA) activity, and this is causally linked to the invasiveness and metastatic behavior of these cells. These findings suggest that the loss of the proto-oncogene Nras in certain cellular contexts can promote malignant tumor progression.     

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.     

Regulation of cellulose synthesis in Acetobacter xylinum by cyclic diguanylic acid

Cellulose is the most abundant renewable carbon resource on earth and is an indispensable raw material for the wood, paper, and textile industries. A model system to study the mechanism of cellulose biogenesis is the bacterium Acetobacter xylinum which produces pure cellulose as an extracellular product. It was from this organism that in vitro preparations which possessed high levels of cellulose synthase activity were first obtained in both membranous and soluble forms. We recently demonstrated that this activity is subject to a complex multi-component regulatory system, in which the synthase is directly affected by an unusual cyclic nucleotide activator enzymatically formed from GTP, and indirectly by a Ca (2+) -sensitive phosphodiesterase which degrades the activator. The cellulose synthase activator (CSA) has now been identified as bis-(3' 5')-cyclic diguanylic acid (5'G3'p5'G3'p) on the basis of mass spectroscopic data, nuclear magnetic resonance analysis and comparison with chemically synthesized material. We also report here on intermediary steps in the synthesis and degradation of this novel circular dinucleotide, which have been integrated into a model for the regulation of cellulose synthesis.     

正交激励超声切削装置的椭圆轨迹偏转与校正

为研究超声椭圆振动切削（ultrasonic elliptical vibration cutting,UEVC）刀尖轨迹形成及控制,解决刀尖轨迹非正椭圆问题,建立二维正交柔性导波UEVC装置刀尖轨迹的运动学模型;分析90°相位差下非正椭圆轨迹产生原因,建立刀尖轨迹校正模型,并推导出基于正椭圆轨迹下的刀尖轨迹偏转模型。通过实验对刀尖轨迹校正模型进行验证,校正后轨迹偏离坐标轴1.67°,小于校正前偏离角度。实验结果表明,该模型能够有效地校正刀尖轨迹,可在轨迹控制方面为后续研究刀尖轨迹偏转及变刀尖轨迹超声振动加工提供理论基础。     

Disruption of mitochondrial function as the basis of the trypanocidal effect of trifluoperazine onTrypanosoma cruzi

Summary The tricyclic anti-calmodulin drug trifluoperazine (TFP) inhibited growth and motility of epimastigotes ofTrypanosoma cruzi, at concentrations lower than 100 M, and motility and infectivity of the bloodstream trypomastigote form at 200 M. Electron microscopy of TFP-treated epimastigotes showed that the major effect was at the mitochondrial level, with gross swelling and disorganization. The oligomycin-sensitive, mitochondrial ATPase was completely inhibited by 20 M TFP, and the same drug concentration caused a 60% decrease in intracellular ATP content. The results suggest that the trypanocidal effect of TFP may be related more to mitochondrial damage than to the well-known anticalmodulin effect of the drug.