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1.
2.
Starch-binding domains in the post-genome era 总被引:1,自引:1,他引:0
Starch belongs to the most abundant biopolymers on Earth. As a source of energy, starch is degraded by a large number of various
amylolytic enzymes. However, only about 10% of them are capable of binding and degrading raw starch. These enzymes usually
possess a distinct sequence-structural module, the so-called starchbinding domain (SBD). In general, all carbohydrate-binding
modules (CBMs) have been classified into the CBM families. In this sequence-based classification the individual types of SBDs
have been placed into seven CBM families: CBM20, CBM21, CBM25, CBM26, CBM34, CBM41 and CBM45. The family CBM20, known also
as a classical C-terminal SBD of microbial amylases, is the most thoroughly studied. The three-dimensional structures have
already been determined by X-ray crystallography or nuclear magnetic resonance for SBDs from five CBM families (20, 25, 26,
34 and 41), and the structure of the CBM21 has been modelled. Despite differences among the amino acid sequences, the fold
of a distorted β-barrel seems to be conserved together with a similar way of substrate binding (mainly stacking interactions
between aromatic residues and glucose rings). SBDs have recently been discovered in many non-amylolytic proteins. These may,
for example, have regulatory functions in starch metabolism in plants or glycogen metabolism in mammals. SBDs have also found
practical uses.
Received 25 May 2006; received after revision 26 June 2006; accepted 3 August 2006 相似文献
3.
该文简述了常用枪口火焰测量的几种方法,对各方法存在的问题下作了系统分析,类比于枪口噪声的测量科学地提出了评价枪口火焰大小及强弱的评价指标,在此基础上提出了一种行之有效的光电测试方法,文中阐明了该方法的原理及系统组成,通过枪口火焰的实弹实验,证明了该方法的可行性。 相似文献
4.
应变速率对8090Al-Li合金拉伸性能的影响 总被引:1,自引:0,他引:1
李慧中 《南京理工大学学报(自然科学版)》1998,(6)
用动态拉伸的方法研究了应变速率对 80 90 Al— L i合金拉伸性能的影响 ,应变速率的范围为 10 - 3~ 10 3s- 1。结果表明 ,合金的强度和塑性随应变率的增加而增加。试样的 SEM和 TEM分析发现 :准静态下的断裂为沿晶断裂 ;随着应变率的增加 ,断裂从穿晶准解理断裂过渡到由显微空穴聚集而成的韧窝断裂。高应变率下80 90 Al- L i合金强度和塑性的提高与其变形机制和晶界无沉淀区 (PFZ)的变化有关 相似文献
5.
F. M. Goñi J. M. Valpuesta M. C. Barbero E. Rial J. I. G. Gurtubay J. M. Macarulla 《Cellular and molecular life sciences : CMLS》1984,40(2):193-195
Summary The solubilizing effect of Triton X-100 on beef heart submitochondrial particles (ETPH) has been studied under various physiological conditions. Coupled, uncoupled and azide-inhibited ETPH particles have been studied. Quantitative and qualitative differences are found in the proteins solubilized by the detergent from ETPH particles under the various conditions tested.Acknowledgments: This work was supported in part by a grant from a Spanish Comisión Asesora para la Investigación Cientifica y Técnica. M.C. Barbero was recipient of a scholarship from the Basque Government. 相似文献
6.
Summary The secondary disease in PF1 strain combination depends upon the immune status of both partners. It is most clearly expressed if both donors and recipients are immunologically crippled by thymectomy and irradiation. Conspicuous reduction in the immune potential of parental parabionts induced by thymectomy and irradiation displayed their marked incidence of parabiotic death. 相似文献
7.
M. Marušić Nikša Allegretti F. Čulo 《Cellular and molecular life sciences : CMLS》1978,34(10):1355-1356
Summary Cyclophosphamide given to rats 2 or 5 days after an injection of Yoshida ascites sarcoma cured approximately the same proportion of animals, but the resistance to a subsequent tumor challenge was found only in rats treated with the drug 5 days after tumor injection. 相似文献
8.
T. Stojanovié B. M. Djuričić B. B. Mršulja 《Cellular and molecular life sciences : CMLS》1980,36(12):1348-1350
Summary The activity of (Na++K+)-ATPase and acetylcholine esterase were folloed in rat brain cerebral cortex, caudate, thalamus, hippocampus and medulla after i.v. administration of physostigmine. Both enzymes were found to be inhibited in a dose-dependent manner. The most pronounced inhibition of (Na++K+)-ATPase was found in caudate. where the highest activity of acetylcholine esterase is found.These studies were supported by a grant from the Union of Science of Republic Serbia, No. 40404-14. 相似文献
9.
S. Bečka J. Bílek J. Slaba J. Škarda I. Mikuláš 《Cellular and molecular life sciences : CMLS》1977,33(6):771-772
Summary Goat placental lactogen was partially purified from a medium collected after placental tissue incubation. The data obtained by disc electrophoresis and isoelectric focusing experiments, as well as by means of radioreceptor assay methods, provide evidence of the similarity between the goat and ovine placental lactogen.The careful technical assistance of L. Tichovská is gratefuly acknowledged. 相似文献
10.
J. Kapitola H. Dlouhá Dr. J. Křeček J. Zicha 《Cellular and molecular life sciences : CMLS》1977,33(12):1615-1616
Summary Neurohypophyseal blood flow increases in water-deprived rats. This increase is independent of vasopressin release, since it occurs even in rats with hereditary defect of hypothalamic vasopressin synthesis.Acknowledgment. The technical assistance of D. Vilimovská is acknowledged. 相似文献