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排序方式: 共有88条查询结果,搜索用时 31 毫秒
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C. Kohlhauser W. Mosgoeller H. Hoeger G. Lubec B. Lubec 《Cellular and molecular life sciences : CMLS》1999,55(11):1491-1501
Perinatal asphyxia (PA) is considered to lead to a variety of brain disorders including spasticity, epilepsy, mental retardation,
and minimal brain disorder syndromes and may form the basis for psychiatric and neurodegenerative diseases later in life.
We examined markers for neuronal transmission involved in the pathomechanisms of PA and candidates as mediators for long-term
sequelae. We tested tyrosine hydroxylase (TH) and the vesicular monoamine transporter (VMAT) representing the monoaminergic
system, the vesicular acetylcholine transporter (VAChT), and the excitatory amino acid carrier 1 (EAAC1), a neuronal subtype
of the glutamate transporter, using immunohistochemistry on brain sections of rats subjected to graded PA. Three months following
the asphyxiant insult immunoreactive (IR)-TH was decreased in striatum, hippocampus, thalamus, frontal cortex, and cerebellum;
IR-VMAT was increased, and IR-VAChT was decreased in striatum. IR-EAAC1 glutamate transporter was increased in frontal cortex.
The cholinergic, monoaminergic, and glutamatergic changes, still observed 3 months after the asphyxiant insult, may reflect
their involvement in the pathomechanisms of PA and indicate mechanisms leading to long-term complications of PA. The variable
consequences on the individual markers in several brain regions may be explained by specific susceptibility of cholinergic,
monoaminergic, and glutamatergic neurons to the asphyxiant insult.
Received 16 March 1999; received after revision 20 May 1999; accepted 8 July 1999 相似文献
3.
粘红酵母处理味精废水的研究 总被引:4,自引:0,他引:4
对粘红酵母处理味精废水的条件进行研究。结果表明最佳处理条件为:进水COD浓度为10000mg/L,pH为6.16,发酵温度为32℃,发酵时间为72h,接种量为15%。该条件下可使味精废水COD的去除率达80.53%以上,利用发酵后的菌体,可提取单细胞蛋白,提取油脂,具有一定的应用潜力。 相似文献
4.
Summary The elucidation of the pathophysiological mechanisms of cerebral ischemia/hypoxia dictates the use of experimental models which mimic this disabling brain condition. In vivo experimental models have been available for many decades and are responsible for the bulk of, though incomplete, knowledge we have about these mechanisms. Since study in isolation of each postulated mechanism is impossible in vivo, the need for an in vitro experimental model has intensified in recent years. Consequently, rat and guinea pig hippocampal slice preparations have emerged as the models of choice. This review attempts to highlight some of the results obtained using brain slices in the study of cerebral ischemia/hypoxia and compare them to those obtained in vivo. Both the biochemical and the physiological correlates of energy metabolism, ion homeostasis, neurotransmission and neuromodulation of this brain condition are reviewed. The agreements, and especially the disagreements, between the in vivo and in vitro findings are emphasized. Details are given of the possible roles of both lactic acid, Ca2+ and excitotoxins in the neuronal damage inflicted by cerebral ischemia/hypoxia. Recent attempts to protect brain slices against experimental cerebral ischemic/hypoxic damage are also reviewed here briefly. 相似文献
5.
Dr. A. Shukla K. N. Agarwal G. S. Shukla 《Cellular and molecular life sciences : CMLS》1989,45(4):343-345
Summary A diet containing 18–20 mg iron/kg to young weaned rats for 8 weeks altered the metabolism of gamma-aminobutyric acid and glutamate in the central nervous system without affecting blood hemoglobin. Subsequent rehabilitation with 390 mg iron/kg diet for 2 weeks normalized these changes. 相似文献
6.
刺激家兔小脑顶核对动脉血压和呼吸的影响 总被引:1,自引:0,他引:1
实验用家兔37只,戊巴比妥钠麻醉,自然呼吸,其中27只采用电刺激小脑项核,10只为顶核内微量注射神经元胞体兴奋剂(L-谷氨酸钠).观察到电刺激小脑顶核能引起动脉血压显著升高,但心电图记录无明显变化;呼吸运动明显加深加快,肺通气量显著增加.而项核内微量注射L-谷氨酸钠.则出现动脉血压降低,心率无明显变化,呼吸也无显著性变化.提示顶核神经元兴奋产全降压作用,而电刺激所致的升压和呼吸反应可能与顶核周围的神经纤维兴奋有关. 相似文献
7.
谷氨酸氧化酶发酵液经(HN4)2SO4沉淀后用透析法获得酶粗品。利用GO测定谷氨酸发酵液中谷氨酸的含量与传统的瓦氏法结果一致,相关系数为0.986,相关方程为:y-0.928x+0.127(其中y,x分别表示氧化酶法与瓦氏法测定的值)。且这种方法比瓦氏法方便、简单、分析成本低、耗时少。利用GO法测定血清中谷草转氨酶和谷丙转氨酶的含量,与传统的试剂盒法比较,相关系数分别为0.978、0.984,相关 相似文献
8.
采用MTT方法观察一定浓度的谷氨酸对PC12细胞活性的影响,结合凝胶电泳和荧光显微镜观察细胞有无DNA断裂和凋亡形态学改变。结果发现谷氨酸确有兴奋毒作用,细胞死亡机制主要为凋亡。 相似文献
9.
M. Bawari G. N. Babu M. M. Ali U. K. Misra S. V. Chandra 《Cellular and molecular life sciences : CMLS》1993,49(12):1092-1094
Glutamate (glu) an excitatory neurotransmitter amino acid, is present in high concentrations in the mammalian central nervous system and is the most abundant amino acid in our daily diet. In the present study the activities of lactate dehydrogenase (LDH) and glutamate dehydrogenase (GDH) were evaluated in the circumventricular organs (CVO) of the brain in 25-day-old rats following MSG administration at a dose of 4 mg/g b.wt during the first ten days of life. The results show the LDH activity increased to 265% of that in the control (p<0.001), whereas GDH activity was significantly decreased (p<0.05), The great elevation in LDH, a cytoplasmic marker enzyme, is apparently due to cytoskeletal changes brought about as a consequence of glu toxicity, whereas lowered GDH activity indicates altered glu homostasis in the blood-brain-barrier deficient areas following neonatal exposure to glu. 相似文献
10.
研究了在味精废水中混合培养粘红酵母和钝顶螺旋藻,并生产油脂。将COD(化学需氧量)为32000mg/L的味精废水稀释5倍,pH值调节到5.5,接种10%粘红酵母,培养3d后接种10%螺旋藻。培养5d后,COD降解率为70.3%,油脂产量为216mg/L,分别是粘红酵母单独培养的1.75倍和5.42倍,螺旋藻单独培养的2.36倍和7.64倍。混合培养也利于废水中NH4+-N、还原糖以及谷氨酸的去除。 相似文献