医学结构基因组学在研制新药中的作用

从药靶的选择、蛋白质片段为基础的新药开发、以寡聚酶为新药开发的靶、工业化的结构和蛋白质数据库以及专门研究传染病的结构基因组学工程等5个方面综述了医学结构基因组学在研制新药中的作用。     

一种改进的分片包标记IP追踪方案

针对Savage等人提出的分片包标记方案(fragment marking scheme,FMS)存在的不足,提出一种改进的分片包标记方案(IFMS),该方案通过扩大标记空间和采用动态标记概率的方法,减少了重构攻击路径所需的数据包数,降低了计算开销,同时采用40位Hash作为误差校验,显著降低了路径重构的误差率.理论分...     

基于MVC分布框架的破片战斗部仿真系统设计

针对破片战斗部起爆至破片着靶整个物理过程进行数值计算与可视化仿真,使用MVC架构、Framework组件库和JSON数据格式等计算机软件技术进行了系统整体设计和实现,实现了系统易扩展、跨平台、易维护特性。并根据用户场景中存在高计算、大数据量、多用户并发等情况设计了分布式计算架构。通过系统仿真实验结果分析,系统分布式仿真性能满足多用户并发需求,可视化仿真效果良好,能为破片战斗部实弹设计与实验提供威力评估依据。     

菲厌氧降解对细菌和古细菌群落的影响

利用TRFLP技术研究了受垃圾渗滤液污染的地下沉积物中细菌和古细菌群落在菲厌氧降解前后的变化。结果表明：细菌群落在生物降解过程中变化很大,物种丰度及Shannon-Weiner指数分别由15和2.39增加到23和2.88;古细菌群落在生物降解过程中变化较小,物种丰度及Shannon-Weiner指数变化不大。     

PRV闽A株gE基因抗原编码区片段在昆虫细胞中的表达研究

伪狂犬病病毒(PRV)糖蛋白E是一种在伪狂犬病的根除计划中具有重要作用的糖蛋白.将伪狂犬病病毒闽A株gE基因抗原编码区片段克隆到杆状病毒Bac-to-Bac系统表达载体pFastBacHTa中,获得的重组表达载体pFastBacHTa-FS转化大肠杆菌DH10BAc感受态细胞后,得到的重组Bacmid DNA在脂质体介导下转染粉蚊夜蛾Hi5细胞,通过细胞内同源重组,获得了含目的片段的重组病毒.此重组病毒感染Hi5细胞后72 h,细胞总蛋白的SDS-PAGE与Western-Blot结果表明,gE基因抗原编码区片段在Hi5细胞中得到了正确表达,表达产物约35000,占细胞总蛋白的9.31%.溶解性分析显示该片段在Hi5细胞中为不可溶性表达.     

Agrobacterium-mediated transformation: state of the art and future prospect

Great progress has been made in recent years in studies on the mechanism ofAgrobacterium-medicated transformation and its application. Many details of the key molecular events within the bacterial cells involved in T-DNA transfer have been elucidated, and it is notable that some plant factors which were elusive before are purified and characterized. Vast kinds of species, which were either recalcitrant to or not included in the host range ofAgrobacterium, can now be transformed by this bacterium, and they include the very important cereal species, gymnosperms, yeast and many filamentous fungi. The simplein vivo transformation of tissue in intact plants and the “agrolistic” methods to transform recalcitrant plants are the two novel technical achievements. Combined with other powerful techniques such as bacterial artificial chromosome, very large DNA fragment can be transformed into the plant genome byAgrobacterium. Further studies will elucidate more plant-encoded factors involved in T-DNA transformation and there is a need to develop more powerfulAgrobacterium-based transformation systems to meet different needs in basic research and crop improvement practice.     

Distribution of tightened end fragments of globular proteins statistically matches that of topohydrophobic positions: towards an efficient punctuation of protein folding?

Using a set of 372 proteins representative of a variety of 56 distinct globular folds, a statistical correlation was observed between two recently revealed features of protein structures: tightened end fragments or 'closed loops', i. e. sequence fragments that are able in three-dimensional (3D) space to nearly close their ends (a current parameter of polymer physics), and 'topohydrophobic positions', i. e. positions always occupied in 3D space by strong hydrophobic amino acids for all members of a fold family. Indeed, in sequence space, the distribution of preferred lengths for tightened end fragments and that for topohydrophobic separation match. In addition to this statistically significant similarity, the extremities of these 'closed loops' may be preferentially occupied by topohydrophobic positions, as observed on a random sample of various folds. This observation may be of special interest for sequence comparison of distantly related proteins. It is also important for the ab initio prediction of protein folds, considering the remarkable topological properties of topohydrophobic positions and their paramount importance within folding nuclei. Consequently, topohydrophobic positions locking the 'closed loops' belong to the deep cores of protein domains and might have a key role in the folding process. Received 1 February 2001; accepted 7 February 2001     

50例中国人IX因子基因的限制性片段长度多态性

用IX因子基因内探针F9(VⅢ)对TaqI,BamHI和EcoRI酶切的50例中国人基因组DNA进行杂交分析。结果表明,所有个体经TaqI酶切的杂交片段为4.5kb和1.8kb,BamHI和EcoRI酶切的杂交片段分别为23kb和5.0 kb。基因组DNA样本中未发现限制性片段长度多态性(RFLP),这与欧美国家的民族群体中存在着IX因子基因内TaqI和BamHI的RFLP的结论不同。造成不同种族间DNA水平差异的原因,很可能与长期在不同地理环境中的进化适应有关。     

k-连通图的可收缩边(英文)

证明了对k-连通图G,若G的任意一个断片满足当N(F)中含有边就有|F|k/4,则G至少有2条可收缩边.