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41.
The plant hormone auxin influences a variety of developmental and physiological processes. But the mechanism of its action is quite unclear. In order to identify and analyze the expression of auxin responsive genes, a cDNA array approach was used to screen for genes with altered expression from Arabidopsis suspension culture after IAA treatment and was identified 50 differentially expressed genes from 13824 cDNA clones. These genes were related to signal transduction, stress responses, senescence, photosynthesis, protein biosynthesis and transportation. The results provide the molecular evidence that auxin influences a variety of physiological processes and pave a way for further investigation of the mechanism of auxin action. Furthermore,we found that the expression of a ClpC (regulation subunit of Clp protease) was repressed by exogenous auxin, but increased in dark-induced senescing leaves. This suggests that ClpC may be a senescence-associated gene and can be regulated by auxin. 相似文献
42.
以MS为基本培养基,附加不同种类、不同浓度的细胞分裂素(BA,6-苄基腺嘌呤,ZT,玉米素)和生长素(NAA,荼乙酸),对月季组培快速繁殖进行了研究。结果表明:在萌芽率上均以较低浓度的细胞分裂素与生长素按一定比例混合使用效果较好,适宜的培养基为MS+BA1mg/L+NAA0.2mg/L.而在成芽率上以较高浓度的细胞分裂素单独使用效果最好,适宜的培养基为MS+ZT2mg/L。 相似文献