Anti tumor Activity of Shark Cartilage Extract and Related Mechanisms *

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Partial Purification and Properties of an Acid Phosphatase from Pearl Oyster Pinctada Fucata

IntroductionAcid phosphatase ( ACP,EC 3.1 .3.2 ) andalkaline phosphatase ( ALP,EC 3.1 .3.1 ) havebeen found to exist widely in the biosphere frombacteria to plants,animals and humans[18] .Thetwo enzymes catalyze the hydrolysis of variousphosphate containing compounds and act astransphosphorylases at acid- and alkaline- p Hvalue.In our lab,an alkaline phosphatase fromthe pearl oyster Pinctada fucata has been purifiedand some of its kinetics has been studied[9] .　Acid phosphatases actas ma…     

Genistein and Daidzein Effects on Proliferation, Cell Membranes, Cell Cycles and Cell Apoptosis of Different Cell Lines

IntroductionIsoflavonoids are found mainly in soybeans,aprimary food source for humans and herbivores.The structure and function of isoflavonoids aresimilar to 1 7β- estradiol (1 7β- E2 ) ,and have dualeffects as both mammal oestrogenicity and anti-oestrogenicity,so they are also termed isoflavonicphytoestrogens(IPE) .It has been reported thatthese compounds can reduce blood fat,as well asthe risk of cancer and osteoporosis[1] .Genistein and daidzein are two principleisoflavonoids in soybe…     

中国二齿蝗属的研究(直翅目:网翅蝗科)

二齿蝗属(Bidentacris Zheng,1982)是网翅蝗科(Arcypteridae)中的一个小属,建立于1982年(郑哲民,1982),该属的种类分布于我国的西南和南部地区。最近,我们在整理本属标本时,发现有三个新种及两个新组合,现将研究结果报导如下。模式标本保存于陕西师范大学生物系蝗虫研究室。     

导弹射击精度鉴定中Bayes方法的运用

以导弹射击精度鉴定问题为对象，研究了运用Bayes理论从仿真试验数据中提取有用信息，从而实现在少量现场试验样本情况下有效完成试验鉴定任务的方法。在简要介绍Bayes理论基本思想的基础上，着重介绍了运用自动（Bootstrap）法从仿真试验结果中获取验前信息的基本思想和一般方法。以及在获得前信息后结合现场试验样本进行导弹射击精度鉴定的方法；最后本文还指出了将Bayes方法应用于具体工程实践时必须考虑的一些问题。     

食蟹猴腹腔补液操作方法及注意事项

随着食蟹猴规模化养殖的迅速发展,其疾病防治问题日益引起大家关注。由于静脉注射在食蟹猴产业化生产中应用具有一定局限性,本文就食蟹猴腹腔补液操作方法和应注意事项做一探讨。     

Effects of Genistein on Cell Cycle and Apoptosis of Two Murine Melanoma Cell Lines

The effects of genistein on several tumor cell lines were investigated to study the effects of gen- istein on cell growth, cell cycle, and apoptosis of two murine melanoma cell lines, B16 and K1735M2. These two closely related murine melanoma cell lines, however, have different responses to the genistein treat- ment. Genistein inhibits the growth of both the B16 and K1735M2 cell lines and arrests the growth at the G2/M phase. After treatment with 60 μmol/L genistein for 72 h, apoptosis and caspase activities were de- tected in B16 cells, while such effects were not found in K1735M2. Further tests showed that after genistein treatment the protein content and mRNA levels of p53 increased in B16, but remained the same in K1735M2. The protein content and mRNA levels of p21WAF1/CIP1 increased in both cell lines after treatment. The results show that genistein might induce apoptosis in B16 cells by damaging the DNA, inhibiting topoi- somerase II, increasing p53 expression, releasing cytochrome c from the mitochondria, and activating the caspases which will lead to apoptosis.     

EDTA对合浦珠母贝碱性磷酸酶活性的影响

以合浦珠母贝(Pinctada fucata)为实验材料,从其外套膜中分离提取碱性磷酸酶(ALP),分别用不同浓度的EDTA不同时间处理ALP,发现随着EDTA浓度的增大,酶的活力指数下降至完全消失,可见酶分子在脱除金属辅基后,活力完全丧失,它是一种金属酶,对酶活性中心金属离子替换实验中。发现分别加入Mg^2 、Mn^2 、Co^2 、Zn^2 后,酶活力可得到一定程度的恢复,而同时加入Mg^2 、Zn^2 ,可使酶活力恢复到92％．     

Characteristics of Two Intermediates Trapped in the Unfolding Pathway of Arginine Kinase Induced by Guanidinium Chloride

Equilibrium guanidinium chloride （GdmCl）-induced unfolding of arginine kinase （AK） was investigated by enzymatic activity, intrinsic fluorescence, 8-anilinonaphthalene-1-sulfonic acid （ANS） fluorescence, circular dichroism （CD） spectrum, and size-exclusion chromatography. The measurements showed that AK unfolded through two equilibrium intermediates： the molten globule state and the partly folded state. Both intermediates have no enzyme activity. The molten globule state exists at 0.4-0.8 mol/L GdmCi, perhaps after the N-terminal domain has unfolded but the C-terminal domain is still intact. The partly folded state occurs at 1.1-1.5 mol/L GdmCI with a hydrodynamic volume no more than 1.6-fold larger than the native state and a pronounced far UV-CD signal. Its ANS fluorescence intensity is about 50% of the molten globule state. This partly folded state shares similarities with the “burst” kinetic intermediate of protein folding.