Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.     

Miniaturized volume holographic optical data storage and correlation system with a storage density of 10 Gb／cm^3

The general idea of holographic optical data storage (HODS) is briefly introduced. Based on the recent advances of HODS, the key techniques and the challenges of HODS are discussed. Some new techniques are proposed to improve the system. A miniaturized volume holographic data storage and correlation system is presented. It can achieve a density of 10 Gb/cm^3 and a fast correlation recognition rate of more than 2000 images per second. It shows the attracting potential advantages over other conventional storage methods in the information storage as well as information processing.     

Role of <Emphasis Type="Italic">N</Emphasis>-linked polymannose oligosaccharides in targeting glycoproteins for endoplasmic reticulum-associated degradation

Misfolded or incompletely assembled multisubunit glycoproteins undergo endoplasmic reticulum-associated degradation (ERAD) regulated in large measure by their N-linked polymannose oligosaccharides. In this quality control system lectin interaction with Glc₃Man₉GlcNAc₂ glycans after trimming with endoplasmic reticulum (ER) -glucosidases and -mannosidases sorts out persistently unfolded glycoproteins for N-deglycosylation and proteolytic degradation. Monoglucosylated (Glc₁Man₉GlcNAc₂) glycoproteins take part in the calnexin/calreticulin glucosylation-deglucosylation cycle, while the Man₈GlcNAc₂ isomer B product of ER mannosidase I interacts with EDEM. Proteasomal degradation requires retrotranslocation into the cytosol through a Sec61 channel and deglycosylation by peptide: N-glycosidase (PNGase); in alternate models both PNGase and proteasomes may be either free in the cytosol or ER membrane-imbedded/attached. Numerous proteins appear to undergo nonproteasomal degradation in which deglycosylation and proteolysis take place in the ER lumen. The released free oligosaccharides (OS) are transported to the cytosol as OS-GlcNAc₂ along with similar components produced by the hydrolytic action of the oligosaccharyltransferase, where they together with OS from the proteasomal pathway are trimmed to Man₅GlcNAc₁ by the action of cytosolic endo--N-acetylglucosaminidase and -mannosidase before entering the lysosomes. Some misfolded glycoproteins can recycle between the ER, intermediate and Golgi compartments, where they are further processed before ERAD. Moreover, properly folded glycoproteins with mannose-trimmed glycans can be deglucosylated in the Golgi by endomannosidase, thereby releasing calreticulin and permitting formation of complex OS. A number of regulatory controls have been described, including the glucosidase-glucosyltransferase shuttle, which controls the level of Glc₃Man₉GlcNAc₂-P-P-Dol, and the unfolded protein response, which enhances synthesis of components of the quality control system.Received 26 January 2004; accepted 25 February 2004     

论企业主素质与民营企业的发展

中国民营企业易“短寿”是内外部多种不良因素造成的,其中企业主素质不高是重要的内因。随着我国社会主义市场经济体制的不断完善和民营企业外部环境的不断改善,民营企业的发展壮大更多的取决于企业的内部因素,尤其是企业主思想文化素质、专业技术水平、经营管理能力和责任意识的提高。     

素质教育观下的翻译教学反思--任务型翻译教学初探

翻译教学作为素质教育的一个重要组成部分,必须体现素质教育三大基本特征主体性、发展性和全面性.以任务型翻译教学模式为例,在翻译教学中,互动任务的完成应以"主题活动"为线索、"合作式学习"为原则,进而达到培养学生翻译能力的目的.     

中学物理教师职前培养的一种新模式

从基础教育改革的重要性和目前发展的十大趋势,论述了对中学教师素质的高新要求．论述了高师人才培养存在培养目标有偏差,人科学与社会科学教育太薄弱,相关专业壁垒未打通,教师专业理论与技能训练严重不足四方面的问题．在高师院校的教学改革中,不仅要强化师范性,还要加强通识教育;重在更新人才培养目标与模式,研制出新的本科教学计划,以求培养出适应素质教育需求的新一代智慧型综合型教师．     

以“三个代表”重要思想为指导优化“两课”教学质量

以“三个代表”重要思想为指导,从增强“两课”教学内容的时代感和实效性的高度,论述了充实“两课”教学内容是提高教学质量的基础;转变教学观念是提高“两课”教学质量的前提;理论联系实际是增强“两课”教学实效性的关键。论述了提高教学质量应增强“两课”教学内容的时代感;提高“两课”教师的整体素质;强化实践教学环节;保证现代教育技术在“两课”教学中的运用。     

普及性音乐素质教育中的评估标准及强化措施

由探索“素质”的词义人手论述“音乐素质”的内涵,针对音乐学习与教育中的某些现实问题进行探讨,强调后天的教育和学习对于提高音乐素质的意义和作用;尝试提出适合于普通高校学生实际的音乐素质评估最低标准;提出强化学生音乐素质的若干措施。     

混合稀土对AB5贮氢合金织构生长特性影响的PLS研究

利用偏最小二乘法（PLS）研究混合稀土组成对贮氢合金的织构生长特性的综合影响,并提出了合金织构择优取向的判据。对于平行于柱状晶织构,择优取向（101） (201)晶面受Nd的影响最为敏感,Ce的影响最小,同时Ce对择优取向的作用与La,Pr,Nd的作用相反;对于垂直柱状晶织构,择优取向（200） (110)晶面受Pr的影响最为敏感,而受Nd的影响最小。     

论大学生素质教育中的"两课"教学模式

从新世纪大学生应具备的素质特征的实际出发,阐述“两课”教学的地位和作用,进一步探索“两课”教学模式,把大学生培养成为对我国改革开放和社会主义现代化建设事业有用的优秀人才。