高粱总DNA导入春小麦新品系高分子量麦谷蛋白亚基的变化

通过花粉管通道法将高粱总DNA导入春麦甘麦8号、陇春13号和陇春10号,经过多代选择获得了5个稳定遗传新品系.在高分子量麦谷蛋白亚基分析中,甘麦8号后代89144的高分子量麦谷蛋白亚基发生突变,较其受体多了5 10亚基,而少了2 12亚基;其他几个转基因后代与其受体比较,高分子量麦谷蛋白亚基组成未发生变化;但是各亚基的相对质量分数有较大变化.高分子量麦谷蛋白亚基的组成和各亚基质量分数的变化直接影响小麦品质.本研究对外源总DNA花粉管通道法导入小麦在改良小麦品质方面的作用进行了讨论.     

TLR9，先天免疫中重要的抗微生物受体

先天免疫系统是宿主抵御外来微生物入侵的第一道防线，TLR9是先天免疫系统中识别细菌和病毒CpGDNA的主要受体。TLR9信号转导利用MyD88依赖途径．在IRAK-4、IRAK-1、TRAF6和TAKl等关键信号蛋白的协同作用下．激活NF-KB和MAP途径，诱导产生一系列促炎细胞因子和趋化因子，最终引起Thl样炎症反应。TLR9除了有抗感染作用，还与自身免疫紊乱和一些恶性肿瘤发生相关。研究TLR9能帮助了解相关疾病发病机理及寻求预防和治疗的手段。     

重组毕氏酵母发酵过程的结构模型

分析了毕氏酵母（Pichia pastoris)以甘油和甲醇为碳源时的代谢途径，建立了胞内物质流和能量流的平衡方程－结构模型。经过适当的降维，解得比碳源消耗速率，比氧消耗速率，比乙酰辅酶A生成速率，细胞比生长速率等关键反应速率。结合反应器模型获得操作变量与过程状态变量间的关系。用实验数据对模型进行了初步验证。结果表明，该模型能较准确地描述细胞生长和重组蛋白合成过程。     

利用目的基因转化技术培育棉花抗病新品种

用花粉管通道法将菜豆几丁质酶基因与烟草β－1、3葡聚糖酶基因双价植物表达载体pBLGC导入新疆棉花主栽品种（系）系550、822、1304、石远321。通过卡那霉素检测、PCR－Southern验证、抗黄萎病、枯萎病性筛选，得到抗病性良好且遗传性稳定的转基因棉花。经过6年10个世代的选育，育成抗枯萎病、抗（耐）黄萎病的转基因抗病新品系。     

生长因子受体介导的信号转导通路研究进展

生长因子受体本身具有酪氨酸激酶活性,它与生长因子结合后发生自动磷酸化并通过酪氨酸磷酸化识别机制作用于含SH2结构域蛋白,启动STATs、Ras、磷脂酶C-γ、磷脂酰肌醇3-激酶、Src激酶等多条胞内信号转导通路.这些通路间的信息交谈和引起基因表达效应的重叠性使它们形成复杂的信号网络系统.     

Effect of UPP on the expression of VEGF and its receptors in mouse uterus during peri-implantation

On day 3 of gestation ,one uterine horn of female pregnant mouse was injected intraluminally with 5 μL 0.1μg/mL lactacystin,a specific inhibitor of ubiquitin-pro-teasome pathway (UPP),while the contralateral horn served as control ,Animals were sacrificed by cervical dislocation on day 5,6,7 of gestation ,respectively,Then the number of implanted embryos in each uterine horn was calcuated,and the expression of VEGF and its receptors was examined,The data showed that the number of implanted embryos was decreased significantly after treatment with lactacystin ,The results of RT-PCR and Western blot indicated that expression of VEGF and its receptors at mRNA and protein levels was significantly decreased in the treated uterus,menawhile,the expression of HIF-1α(the α subunit of HIF ,a transcrip-tional factor of VEGF) was reduced at both mRNA and protein levels,These data suggested that the effect of UPP on VEGF expression was realized through regulating HIF-1α expression .In addition ,UPP is likely to take part in the modulation of VEGF receptors expression ,These changes may be one of the reasons for the reduction of implanted embryos.     

Learning figurative idioms via cognitive semantic pathway

In FTL contexts,traditional view treats idiomatic language as essentially arbitrary,which has typically led to the belief that they can only be learned through blind memoriztion. However,the cognitive semantic idea considers that idioms are typically motivated,which can help learners to identify their senses. This paper demonstrates how to learn figurative idioms through cognitive semantic pathway by taking anger as one example.     

乌北南二段输导层油气运移优势路径演化研究

根据输导层内油气运移优势路径的形态特征,将油气运移优势路径划分为汇聚型、发散型和平行型3种形式。通过乌尔逊凹陷北部南二段输导层特征分析,在古埋深恢复的基础上,利用油气运移优势路径研究原理和方法对乌尔逊凹陷北部南二段输导层各地质时期的油气运移优势路径进行了研究。得到该区各地质时期南二段输导层油气运移优势路径的分布具有相似特征,主要有5个汇聚区,以苏1井、苏21井和乌8井汇聚区油气运移优势路径密度大,苏21井汇聚区位于生烃灶的边部,苏1井和乌8井汇聚区位于生烃灶内,有利于油气的运聚成藏。西部两个汇聚区油气运移优势路径密度小,且位于生烃灶之外,不利于油气聚集成藏。该区目前的工业油气流井不是分布在油气运移优势路径的汇聚点处,就是在其优势路径上,这是该区南二段油气成藏的重要条件。     

硒的生物循环及含硒活性物质研究进展

概述硒的生物循环和含硒活性物质研究的进展。