Computer simulation of flow fields for quenching medium

Using finite element method (FEM), the complex flow fields of quenching medium in a quenching tank are calculated for the cases with and without a work-piece in it. The influence of the flow homogenization device on the flow fields is studied. The computer simulation results are in consistency with the actual measurement results. According to the simulated results, the structure of the quenching tank and the flow homogenization device are optimized and more uniform flow fields are obtained.     

3-甲基-6-氨基-5-氰基-4-（4-对甲氧基）苯基-1-苯基-1，4-二氢吡喃并[2，3-c]吡唑与牛血清白蛋白相互作用的研究

采用荧光光谱法研究了人体生理pH值条件下,3-甲基-6-氨基-5-氰基-4-(4-对甲氧基)苯基-1-苯基-1,4-二氢吡喃并[2,3-c]吡唑(Ⅰ)与牛血清白蛋白(BSA)分子间的相互结合反应;获得了不同温度下Ⅰ与BSA作用的结合常数K和结合位点数n;并根据Forster非辐射能量转移机理,计算出其与BSA相互作用时给体-受体间距离r为5.01 nm及能量转移效率E为0.280.证实了Ⅰ与BSA的相互结合作用为单一的静态猝灭过程,确定了其与BSA分子间有较强的结合作用,且结合力以疏水作用力为主.     

甲基红与牛血清白蛋白结合的光谱研究

牛血清白蛋白在不同pH的溶液中存在N(Native,pH～7.0),B(Basic,pH～9.0),E(Expanded,pH3.5以下)等几种结构形态.用光谱技术研究了染料甲基红(Methyl red,MR)和BSA的结合机理,研究了MR和BSA在不同pH的三酸缓冲溶液中的结构和构象变化,荧光猝灭方法测得两者在pH3.0,4.9,7.0,9.0溶液中结合常数分别为7.544×105 L/mol,7.594×105 L/mol,4.728×105 L/mol,4.880×105 L/mol;结合位点数分别为17.89,0.978 3,3.079,8.865.同步荧光显示MR加入对Tyr残基微环境没有影响,但对Trp残基产生微扰,使其最大发射波长发生蓝移.图5,表1,参20.     

SPV490钢板直接淬火回火工艺的研究

针对SPV490钢控轧后的直接淬火回火工艺进行研究.结果表明,采用再结晶区控轧后结合直接淬火回火工艺时,实验钢的强度大幅度提高,但韧性下降;采用在奥氏体再结晶区变形44%,然后在未再结晶区变形的两阶段控轧工艺后结合直接淬火回火工艺时,由于在细化晶粒的基础上增加晶粒内部变形带数量及位错密度,从而获得细小、均匀的组织,实验钢的综合力学性能良好.     

光谱法研究一种含咪唑啉酮类衍生物与牛血清白蛋白的相互作用

应用荧光光谱及紫外可见光谱的方法研究了2-氨基-5-苯亚甲基-4H-咪唑啉-4-酮衍生物(BPH5)与牛血清白蛋白(BSA)的相互作用.实验发现BPH5能强烈猝灭牛血清白蛋白的荧光强度,其荧光猝灭机理为动态猝灭.在此基础上计算了二者相互作用的结合常数、结合位点数及△H,△G,△S等热力学参数等.结果表明BPH5与BSA以1∶1结合,其反应主要是熵驱动的,相互作用力主要为疏水作用力.根据F(o)rster无辐射能量转移理论计算了给体(BSA)与受体(BPH5)之间的结合距离.     

荧光法研究氟罗沙星与牛血清白蛋白的相互作用

用荧光法研究了氟罗沙星对血清白蛋白的荧光猝灭现象，根据荧光猝灭双倒数图读物计算了氟罗沙星与牛血清白蛋白之间的结合常数，根据Foester能量传递原理计算出氟罗沙星在牛血清白蛋白上的结合位置，并根据热力学参数研究了氟罗沙星与牛血清白蛋白之间的作用力类型。     

Relationships between D1 protein, xanthophyll cycle and photodamage-resistant capacity in rice (Orysa sativa L.)

Relationships between D1 protein, xanthophyll cycle and subspecific difference of photodamage-resistant capacity have been studied in O. japonica rice varieties 02428 and 029 (photoinhibition-tolerance) and O. indica rice varieties 3037 and Palghar (photoinhibition-sensitivity) and their reciprocal cross F1 hybrids after photoinhibitory treatment. It was shown that PSⅡ photochemical efficiency (Fv /Fm) decreased, and xanthophyll cycle from violaxanthin (V), via anaxanthin (A), to zeaxanthin (Z) was enhanced and non-photochemical quenching (qN) increased accordingly in SM-pretreated leaves of rice when the synthesis of D1 protein was inhibited, and that there was a decrease in qN and, as a result, more loss of D1 protein and a big decrease in Fv /Fm in DTT-pretreated leaves when xanthophyll cycle was inhibited. O. japonica subspecies had a higher maintaining capacity of D1 protein and a decrease of Fv /Fm in a more narrow range, and exhibited more resistance against photodamage, as compared with O. indica subspecies. The above physiological indexes in reciprocal cross F1 hybrids, though between the values of their parents, were closer to maternal lines than to paternal lines. Experimental results support the concept that the turnover capacity for D1 protein is an important physiological basis of photoinhibition-tolerance, and will provide the physiological basis for selection of the photoinhibition-tolerant parents and develop a new approach to breed hybrids with high photosynthetic efficiency.     

Abnormal behavior of fluorescence quenching of gramicidin A inphase transition of lipid vesicles by hypocrellin B——Does anelectron transfer inverted region exist?

The fluorescence of gramicidin A(GA) in lipid bilayer may be quenched by Hypocrellin B. When the phospholipids undergo a phase transition from gel to liquid crystalline phase, the reaction rate decreases unusually. This phenomenon has been analyzed by Marcus’ photoinduced electron theory, and an electron transfer inverted region hypothesis is proposed, which may adequately explain this abnormal phenomenon.     

大模数人字齿轮轴中频表淬质量探讨

文中分析了大模数人字齿轮轴中频表淬后，出现齿面硬度不足，不均和裂纹等影响质量问题的原因，提出了改进大模数人字齿轮轴中频表淬质量的措施。     

薄壁齿轮及薄大齿轮渗碳淬火变形问题的研究

对薄壁的齿轮及薄大齿轮渗碳淬火变形及影响因素进行了和研究并提出了解决此类零件变形的方法。