数字图书馆版权适度保护的研究现状及原则分析

归纳总结了国内外有关涉及到数字图书馆的版权保护的立法及学术研究现状.针对版权强化保护及弱化保护的两种观点,作者提出了适度保护的思想,重点阐述了数字图书馆版权适度保护应当遵循的利益平衡原则、系统思考原则和社会受益原则.     

轴压单孔冷弯薄壁槽形中长钢柱的极限承载力

为研究腹板开孔的冷弯薄壁构件极限承载力，选取两端简支、轴心受压、腹板开单孔的冷弯槽形中长钢柱进行非线性有限元分析研究，通过对试件的变形及竖向位移-荷载曲线的分析，表明开孔构件的竖向刚度和极限承载力较无孔构件降低．有限元模拟与试验吻合较好．结果表明，选择合理的有限元数值模型，可以较好地模拟开孔钢柱的受力性能．根据数值分析结果，对开孔钢构件的畸变屈曲特征进行了分析，同时也分析了孔洞高度、宽度对构件极限承栽力的影响．     

MODIS在青藏高原大范围积雪制图中的应用及存在的问题

积雪是地球表面重要的组成部分之一,精确监测积雪覆盖及其动态变化是地球科学的一个重要研究方向。本文以我国主要积雪区之一的青藏高原作为研究区域,研究了MODIS数据在青藏高原大范围积雪制图中的应用。使用基于NDSI阈值法的积雪像元识别方法,对2002-2003年度青藏高原的积雪状况进行了监测。对监测结果的分析表明,虽然这种算法目前还存在一些不足,但对青藏高原大区域的积雪监测仍是一种极为有效的方法。     

Monitoring of Cerebral Oxygenation by Near-infrared Spectroscopy during Cardiopulmonary Bypass in Infants

Objective： To evaluate the use of near-infrared spectroscopy for monitoring cerebral oxygenation under different cardiopulmonary bypass models. Method： Twenty-four patients with ventricular septal defect and pulmonary hypertension undergoing open-heart surgery were assigned eight each to three groups, with respect to different cardiopulmonary bypass models： moderate hypothennia cardiopulmonary bypass, deep hypothennia low flow and deep hypothennia circulatory arrest. For each patient, cerebral oxygenation with near-infrared spectroscopy were monitored and the relative concentration changes in cerebral oxygenated hemoglobin, deoxygenated hemoglobin and oxidized cytochrome aa3 were calculated. Electroencephalography, biochemical indicators such as neuron-specific enolase and lactate, and performed correlation analyses for nearinfrared spectroscopy data and biochemical indicators were also measured. Results： Near-infrared spectroscopy data and biochemical indicators for moderate hypothennia cardiopulmonary bypass and deep hypothennia low flow group showed no correlation. For deep hypothennia circulatory arrest group, oxygenated hemoglobin signal declined to a plateau （nadir） during the circulatory arrest period. The duration from reaching nadir until reperfusion ＂oxygenated hemoglobin signal nadir-time＂, and the minimum values of oxygenated hemoglobin, and oxidized cytochrome aa3 were closely correlated with increasing neuron-specific enolase and lactate. And, all patients whose oxygenated hemoglobin signal nadir-time was less than 35 rain were free from behavioral evidence of brain injury. Conclusion： Near-infrared spectroscopy data including oxygenated hemoglobin signal nadir-time and the minimum of oxygenated hemoglobin and oxidized cytochrome aa3 showed strong correlation with other cerebral function assessment for deep hypothermia circulatory arrest. Oxygenated hemoglobin signal nadir-time determined by near-infrared spectroscopy can be useful in predicting the safe duration of circulatory arrest.     

嗜热侧孢霉2441纤维素酶产酶条件优化及突变株选育

为了提高嗜热侧孢霉2441纤维素酶的产量,对产酶条件进行了优化,测定了酶的特性,对选育抗葡萄糖效应突变株和中温型突变株进行了探索.实验中酶活力测定以微晶纤维素、CMC-Na和水杨苷为底物,DNS法测定还原糖;产酶条件优化采用正交试验设计,以微晶纤维素酶活为指标;通过NTG诱变和UV诱变选育抗葡萄糖效应突变株和中温型突变株.结果表明:产酶条件优化后,嗜热侧孢霉2441的纤维素酶活极显著提高(P0.01);2441的微晶纤维素酶、CMC-Na酶和β-葡萄糖苷酶的最适作用温度分别为60℃、65℃、70℃,最适作用pH值均为5.5,温度高于70℃及碱性条件下,酶活力显著下降;选育的抗葡萄糖效应突变株N20-01,微晶纤维素酶活提高13.203%,CMC-Na酶活提高47.176%,β-葡萄糖苷酶活提高119.517%;选育的中温型突变株U40-03,在32℃培养时与2441在45℃培养时相比,微晶纤维素酶活提高11.411%,CMC-Na酶活提高29.484%,β-葡萄糖苷酶活提高54.759%.     

THE MODERATE LONGHOLE BLASTING FRAGMENTATION CONTROL TECHNOLOGY RESEARCH IN UNDERGROUND MINE

The scallop moderate longhole blasting mining method in Da Hong-shan copper mine applications accounted for more than 61% of the total amount of mining, but the large output rate restricts the mines for ore strength, The average lump ore as high as 22.7%. In order to realize the mine production efficient development, the circular moderate longhole blasting technology and field test. The test results show that circular hole blasting can reduce the block rate is reduced to 10.3%, and the traditional scallop moderate longhole blasting mining compared with the average lump ore decreased by 12.4 percentage points. The panel on duty for the day ore quantity increase 152.31t, increase rate is 51.1%. Reduces the handling of large time delay and secondary crushing of explosives and the consumption of detonators, the efficiency of drawing and at the same time, reduce the mining cost , achieved good blasting effect.     

粘弹性地基上四边自由矩形中厚板的非线性自由振动分析

为了解决中厚板与粘弹性地基共同作用下的非线性振动问题,在Reissner-Mindlin一阶剪切变形板的理论基础上,运用pb-2瑞利-里兹法分析双参数粘弹性地基上四边自由矩形中厚板的非线性自由振动,探讨了板的尺寸参数、横向剪切因子、粘滞系数以及地基反应模量对板的振动特性产生的影响及其变化规律。并对数值算例进行编程求解,与文献实测数据进行对比,结果十分接近,证明了该方法的可行性。     

不同来源CIK与DC细胞联合治疗中晚期肺癌的临床研究

为对癌症患者采用个体化治疗,用患者自体、同种异体外周血和脐血进行细胞因子诱导的杀伤细胞(Cytokins Induced Killer,CIK)及树突状细胞《Dendritic Cell,DC)扩增,并应用流式细胞术检测其CIK、DC-CIK表型,然后回输患者,对中晚期肺癌患者进行自体、异体和脐血的CIK细胞联合治疗。结果显示:21例患者中,2例病情得到完全缓解(CR),7例病情部分缓解(PR),与治疗前相比,生活质量明显提高,治疗后患者CD3~ 和CD8~ 明显升高(P<0.05)。结果证明,根据不同病人情况采用个体化治疗,应用不同来源的CIK、DC-CIK治疗中晚期肺癌有一定的临床疗效,该方法为中晚期肺癌患者的治疗提供了一种有效的免疫治疗手段。     

中度嗜盐菌GbsB基因的克隆、序列分析及生物信息学分析

为构建耐盐的转基因植物提供材料,根据地衣芽孢杆菌Bacillus licheniformisATCC 14580的GbsB基因的核甘酸序列设计1对特异性引物,通过PCR的方法扩增由分离的中度嗜盐菌Bacillus sp.XJ1-05的GbsB基因,经T/A克隆,插入到pBS-T载体上进行序列测序,采用NCBI-BlastX软件在Genbank数据库中进行同源性检索,结果表明:得到的GbsB基因的开放阅读框(ORF)全长为1209bp,编码1个由402个氨基酸残基组成的蛋白质;其与Bacillus licheni-formisATCC 14580的GbsB基因的氨基酸序列同源性高达96%。生物信息学分析表明:该GbsB基因编码蛋白无信号肽,无跨膜区域,高亲水性,有2个乙醇脱氢酶作用位点。     

腾冲热海嗜热芽孢杆菌的分离鉴定

从云南腾冲热海温泉中分离得到26株嗜热芽孢杆菌菌株,对其中一株嗜热芽孢杆菌菌株NHH4进行电镜形态、生长特征、碳源、氮源等分析。该菌株细胞形态为杆状,产芽胞,革兰氏染色阳性,严格好氧,其最适生长温度为55℃,最适为pH7.5。能利用葡萄糖、蔗糖、D-果糖、D-甘露糖,不能利用麦芽糖、乳糖、D-木糖、L-鼠季糖。通过对其16S rRNA基因序列的系统发育分析表明,NHH4与Bacillus licheniformis strain N8的序列相似性为99%,将此菌株鉴定为嗜热地衣芽胞杆菌菌株。