灵芝与中药共发酵产物的药理学初步研究

为考察灵芝培养基在添加不同中药后，其发酵液药理作用的变化，选择小鼠负重游泳实验，戊巴比妥钠睡眠时间试验，小鼠断头后致急性脑缺氧试验，以及对S180荷瘤小鼠瘤重的影响，研究在灵芝培养基中添加不同中药后其发酵液的抗疲劳、改善睡眠、耐缺氧与抑制肿瘤作用，并研究了这几味中药对灵芝生长的影响.结果发现在灵芝培养基中添加黄芪、姜黄、补骨脂、红毛五加后，其发酵液能明显延长小鼠负重游泳时间；在培养基中添加黄芪、红毛五加的发酵液对小鼠急性脑缺血性缺氧具有较好的保护作用；在培养基中添加巴戟天后，其发酵液灌胃的小鼠，瘤重减轻.表明在灵芝培养基中添加中药后，会改变灵芝发酵液的药理作用.     

油樟叶内生真菌的多样性研究

 采取在马铃薯培养基上加无菌油樟(Cinnamomum longepaniculatum N.Chao ex H.W.Li)叶汁液作为分离培养基的办法,从油樟树叶中分离到24株内生真菌,经过鉴定,其分属于子囊菌纲的双足囊菌属(Dipodascus Lagerh.)、拟指突孢曲霉属(Emericellopsis J.F.H.Beyma)和半知菌类的花核菌属(Anthina Fr.)、青霉属(Penicillium Link)、组丝核菌属(Phacodium Pers.)、茎叶核菌属(Ectostroma Fr.)、地霉(Geotrichum Link)、皮核菌(Acinula Fr.)、团丝核菌(Papulaspora Preuss)、发菌(Capillaria Pers.)、峡串孢霉(Paepalopsis J.G.Khn)、小卵孢霉(Ovularia Sacc.)、卵形孢霉(Oospora Wallr.)、稀丝头孢霉(Haplotrichum Link)、单梗曲霉(Briarea Corda)等15个属,且以无孢菌居多,表现出较为丰富的类群(物种)多样性.     

亚麻脱胶菌的分离、筛选和鉴定

 从云南亚麻沤麻水、种植土、沤麻残渣堆积土中粗筛获得具有果胶酶活性的菌株51株;通过几种酶活性测定,获得果胶酶和木聚糖酶(主要的半纤维素酶)活性较高、无纤维素酶活力且脱胶周期短的目的菌株4株.基于4个菌株的果胶分解能力、沤麻周期长短以及沤麻的效果等指标,最终确认YN1.1是优良的亚麻脱胶菌株.经16S rRNA基因测序和生理生化鉴定,该株菌为芽胞杆菌属的地衣芽孢杆菌.     

Biomineralization of phototrophic microbes in silica-enriched hot springs in South China

Microbial mats in two hot springs in South China were sampled for the research of mineralization of microbes and its mechanism by the methods of geology and modern biology. The results show that hot spring microbes have the key capability for enrichment of Si, Al, Fe, Ca and other elements, and the microbes are also crucial for the formation of SiO2, CaCO3, clay and so on. The extracellular polymeric substances (EPS) play important roles in the process of mineralization of hot spring microbes, which mainly takes place in the layer of EPS outside cell wall or sheath of cyanobacteria. The sheath outside cell wall, which keeps the normal metabolism of cyanobacteria during the process of mineralization on its surface, is also considerable for the biomineralization of cyanobacteria. According to structure and mineralization characteristics of two microbial mats, the process of mineralization can be divided into three stages, namely, early surface mineralization, middle degradation mineralization, and late des- quamation of mineral. The above conclusions are significant for comprehension of the process of mineralization, the process of deposition and the preservation of microfossil in modern and ancient extreme environments.     

微生物絮凝剂的应用研究

以处理高岭土悬浊液和池底污泥配制成的废液为基础,测定了其絮凝性,得出各种废水的最佳处理条件,探讨了金属阳离子对微生物絮凝性的影响。     

绿僵菌菌剂中试生产基地建设

介绍了国内外绿僵菌资源的开发现状以及山西省在绿僵菌研究方面所做的工作,阐述了绿僵菌菌剂中试生产基地建设项目的计划目标、主要研究内容、实施年限、经费预算、必要的支撑条件、组织措施及实施方案。     

Microbial communities in microcosm soils treated with battery waste

Battery waste is one of the most destructive hazards to our environment,especially to the soil.In order to understand the effects of the battery waste on the microbial communities in soil,microcosm soils were treated with the powder made from the battery waste.Microbial biomass and respiration were measured after 15,30,45,and 60 days of the treatment,and catabolic capability and Bi- olog profile were determined after 60 days.Microbial biomass was declined by all treatments,while microbial respiration and catabolic ca- pability were enhanced.Although microbial biomass recovered after a period of incubation,microbial respiratory quotient,catabolic capa- bility and community structure remained significantly affected.Our results also suggest that microbial respiratory quotient and Biolog pa- rameters are more sensitive than microbial biomass to the battery stress on bioavailability.     

产絮凝剂双菌复合发酵体系的研究以及在水处理中的应用

从土壤和活性污泥中筛选到三株絮凝剂产生菌,将其进行双菌混合培养的正交试验,构建出了比单一菌所产絮凝剂絮凝活性更高的复合菌群LH,通过16SrRNA序列分析,初步判定二菌分别为Micrococcus sp.和Paenibacill usvelaei菌.对其性质的初步研究发现,复合菌LH所产絮凝剂MBF-LH为胞外代谢产物,其热稳定性好.LH产絮凝剂的最适碳源、最适培养温度、最适pH值和通气量分别为蔗糖、30℃、pH8和120r/min恒温振荡培养.培养基中添加KCl、CaSO4、K2HPO4能明显提高发酵液絮凝率.絮凝剂MBF-LH处理废水时具有用量少、絮凝率高的特点.     

微生物生态学研究进展——第十一届国际微生物生态学大会综述

第11届国际微生物生态学会于2006年8月在奥地利维也纳召开。此次会议以“隐藏的力量——行动中的微生物群落”为主题,特邀了当今世界在微生物生态学领域最为著名的科学家作专题报告,内容广博,反映了当今微生物生态学研究的发展动向。本文简要介绍了第十一届国际微生物生态学大会的概况和部分演讲摘要。     

微生物对地表水中石油类污染物的降解研究

针对当前地表水油污染具有面积、低浓度的特点，应用筛选高效降油微生物除油。试验结果表明，投加除油混合菌群，适当充氧，经２４ｈ处理，水中石油类污染物去除率达９０％以上。同时对不同种类油污染物的降解进行了初步研究。