Study of transmembrane La3+ movement in rat ventricular myocytes by the patch-clamp technique

We have studied transmembrane La3+ movement in rat ventricular myocytes for the first time by using the whole-cell patch-clamp recording mode. La3+ (0.01-5.0 mmol/L) could not bring out inward currents through the L-type calcium channel in rat ventricular myocytes, while it could enter the cells by the same way carried by 1μmol/L ionomycin. When the outward Na+ concentration gradient is formed, La3+ can enter the cells via Na-Ca exchange, and the exchange currentsincrease with the increase of external La3+ concentrations. But compared with Na-Ca exchange currents in the same concentration, the former is only 14%-38% of the latter. The patch-clamp experiment indicates that La3+ normally can not enter ventricular myocytes through L-type calcium channel, but it can enter the cells via Na-Ca exchange.     

弧焊电源动特性微机测试仪

本文介绍了一种由MCS-51系列单片机构成的数据采集系统以及用于电焊机动特性的测试软件。采用PP-40绘图打印机可打印出焊机动特性特征参数,并可描绘焊接电流、电压的动态波形。从而解决了《弧焊电源》课程实验和直流焊机动特性评定中存在的问题。     

Effect of Calcium on Spontaneous Quantal Transmitter Secretion from ACh-Loaded Myocytes

Introduction Transmitter secretion requires specialized secretory or- ganelles, the synaptic vesicles, for the packaging, stor- age, and exocytotic release of the transmitters[1,2]. The neurotransmitter acetylcholine (ACh) is released at the neuromuscular…     

Dynamics of proteins in Golgi membranes: comparisons between mammalian and plant cells highlighted by photobleaching techniques

In less than a decade the green fluorescent protein (GFP) has become one of the most popular tools for cell biologists for the study of dynamic processes in vivo. GFP has revolutionised the scientific approach for the study of vital organelles, such as the Golgi apparatus. As Golgi proteins can be tagged with GFP, in most cases without altering their targeting and function, it is a great substitute to conventional dyes used in the past to highlight this compartment. In this review, we cover the application of GFP and its spectral derivatives in the study of Golgi dynamics in mammalian and plant cells. In particular, we focus on the technique of selective photobleaching known as fluorescence recovery after photobleaching, which has successfully shed light on essential differences in the biology of the Golgi apparatus in mammalian and plant cells.     

芽孢杆菌植酸酶基因在毕赤酵母中的胞内表达

根据已知的枯草芽孢杆菌WHNB02植酸酶phyC基因全序列设计一对引物,采用PCR法从含有该基因的pUC18-phyC质粒上获得了长约1.1kb不含有信号肽序列的植酸酶phyC基因表达片段.经T载体克隆及序列测定后,构建毕赤巴斯德表达载体pPIC3.5K-phyC,并电转化毕赤巴斯德酵母宿主菌GS115.经MD和MM平板筛选、酶活性测定,获得了阳性转化子,并进行了诱导表达.SDS-PAGE分析表明:表达产物分子量为42.01KD,表达量占细胞可溶性总蛋白的24%,并具有植酸酶的生物学活性.酶学性质分析结果显示:胞内表达的植酸酶酶促反应最适pH值为7.5;最适反应温度为70℃;经90℃处理10min,残留酶活性42% 均优于出发菌株天然植酸酶的相应性质.     

Icariin suppresses bone resorption activity of rabbit osteoclasts in vitro

The effect of icariin on the bone resorption activity of rabbit osteoclasts is assessed in vitro. Osteoclasts were isolated from Japanese white rabbits and cultured on plates with a sterilized bone slice in each well. After treatment with icariin at various concentrations, the bone resorption activity of osteoclasts was evaluated by examining pit areas, superoxide anion (·O2-) generation, size and number of actin rings and intracellular calcium concentration [Ca2 ]i. As revealed by these data, icariin elicited continuous decline of [Ca2 ]i, making actin ring constricted and ·O2- generation decreased. These events resulted in smaller and fewer pits which indicate suppressed bone resorption activity of rabbit osteoclasts by icariin.     

正交化经验模式分解方法

针对Hilbert—Huang变换中经验模式分解所得固有模式函数存在的不完全正交问题，基于Gram-Schmidt正交化方法，提出了一种新的正交化处理方法，可得到完全正交的固有模式函数，从而完善了Hilbert—Huang变换方法．通过典型时程曲线的数值模拟的分解结果表明了这一方法的正确性，实际地震波数据的分解显示了这一方法的良好应用前景．     

一种N/(N+1)型高码率游长受限码编码方法

基于数据分块的分布特性,提出了一种可用于磁存储或光存储读写通道中的高码率游长受限码的编码方法,包括对数据进行子块分割和检测、建立标志子块队列,按照排队规则和无冲突子块的映射策略进行码字映射等.该方法能在给定的(d,k)受限条件下接近编码容量,并具有递推设计更高码率编码的扩展性能.利用该方法设计了码率为32/33(d=0,k=6)的游长受限码,并给出了结合子块交叉插入技巧设计64/65(0,10)游长受限码的方法,还对受限参数与码率和编码容量的关系进行了探讨.     

“旂铃”与“圣人振铎”神话——从文化大传统探寻“工史”如何“书世”

当下人文社科研究的人类学和考古学两大转向语境下,书契与讽诵作为四重证据和文化大传统的重新发现,为因筛选、遮蔽和遗忘而导致的文字文献难解谜题提供了释读新视界。＂工史书世＂的基本方式,是工史身份递降和人类文明传承方式及技术变迁的物证。后世王者所追认的圣王谱系和神性祖先,原本是天神之子或可以借助神赐法器变形幻游通神的宗教领袖。职司书世与诗世的史官用事之物旂铃、玉瑹和圣人振铎、凤鸣衔珪等圣瑞建构均深植于神圣传统和法器信仰神话。     

飞参数据地面处理仿真系统研究

飞参数据地面处理仿真系统研究是综合运用计算机仿真的原理和技术而做的飞行参数计算机模拟仿真系统。在仿真系统设计中提出了飞行参数仿真系统的结构、仿真系统内部的数据流程管理结构及其管理方法。仿真针对飞参数据提出了通用的译码算法和飞参数据二进制文件的处理方法及仿真误差的校验方法。