Targeted inhibition of Livin resensitizes renal cancer cells towards apoptosis

Cancer cells are typically characterized by apoptosis deficiency. In order to investigate a possible role for the anti-apoptotic livin gene in renal cell cancer (RCC), we analyzed its expression in tumor tissue samples and in RCC-derived cell lines. In addition, we studied the contribution of livin to the apoptotic resistance of RCC cells by RNA interference (RNAi). Livin gene expression was detected in a significant portion of RCC tumor tissue specimens (13/14, 92.9%) and tumor-derived cell lines (12/15, 80.0%). Moreover, targeted inhibition of livin by RNAi markedly sensitized RCC cells towards proapoptotic stimuli, such as UV irradiation or the chemotherapeutic drugs etoposide, 5-fluorouracil, and vinblastine. These effects were specific for livin expressing tumor cells. We conclude that livin can contribute significantly to the apoptosis resistance of RCC cells. Targeted inhibition of livin could represent a novel therapeutic strategy to increase the sensitivity of renal cancers towards pro-apoptotic agents. Received 30 November 2006; received after revision 22 February 2007; accepted 20 March 2007     

酸洗抑雾缓蚀剂的研制

带有废酸脱硅再生系统的酸洗机组对于所用抑雾缓蚀剂有特殊的要求。它要求所用抑雾缓蚀剂既具有高的抑雾效果，其缓蚀率又要适当；既不影响脱硅和铁红质量，更不能对系统造成堵塞和影响系统物料的平衡。针对上述要求研制出特效的酸洗抑雾缓蚀剂。     

Purification of platelet-derived endothelial cell growth inhibitor and its characterization as transforming growth factor-β type 1

In 1986, Brown and Clemmons (Proc. natl Acad. Sci. USA83 (1986) 3321) showed that platelets contain a substance, platelet-derived growth inhibitor (PDGI), that inhibits in vitro endothelial cell replication. Although platelets are rich in transforming grwoth factor (TGF-), PDGI was considered not to be related to TGF-, on the basis of its reported properties (extraction from platelets at neutral pH, binding to heparin-Sepharose). However, we purified PDGI to near homogeneity and showed that on the basis of HPLC retention behavior, in vitro growth inhibitory activities with several cell types, receptor binding, and immunoneutralization of growth inhibitory activity with specific anti-TGF- type 1 antibodies, PDGI is most probably identical with TGF- type 1.     

Cooperative inhibitory effect of follicular fluid and cAMP on hamster oocyte maturation

Summary Porcine or human follicular fluid inhibited the spontaneous maturation of isolated hamster oocytes in vitro during the first 1.5 h of culture. Moreover, the presence of 50% follicular fluid combined with 100 M dbcAMP cooperatively reduced the incidence of germinal vesicle breakdown. The addition of FSH also inhibited the resumption of meiosis, and the presence of LH did not overcome the inhibitory effects of follicular fluid and tended to impede isolated hamster oocyte maturation in vitro.     

含钼杂多酸盐对碳钢缓蚀性能的研究

采用失重法考察了６种具有Ｋｅｇｇｉｎ结构的含钼杂多酸盐在ＮａＣｌ介质中对碳钢的缓蚀作用。实验表明,所研究的化合物均有一定的缓蚀性能,尤其抑制点蚀效果很好。     

低频超声波对大豆胰蛋白酶抑制素的处理效果

采用２０ｋＨｚ的低频超声波对大豆胰蛋白酶抑制素（ＳＴＩ）进行了处理。结果表明,超声波对经纯化了的鲍曼－贝尔克抑制素（ＢＢＩ）几乎不起钝化作用,对生豆奶中天然存在的ＳＴＩ的钝化效果要比对从大豆中提取纯化的ＳＴＩ好得多,说明纯化了的ＳＴＩ似对超声处理较稳定;豆奶中大分子蛋白组分１１Ｓ和７Ｓ的存在有助地ＳＴＩ的纯化;低频超声波对生豆奶中的ＢＢＩ似乎有轻微的激活作用;在生豆奶中添加半胱氨酸、亚硫酸钠的胃液     

苯并咪唑类化合物作为碳钢缓蚀剂的研究

通过失质量法、极化曲线法研究了20^#碳钢在含有苯并咪唑类化合物的HCL溶液中的腐蚀行为。结果发现在室温和50℃的条件下，5种苯并咪唑化合物的缓蚀效果都很明显。其中2-对氯苄基苯并咪唑的缓蚀性能最好。     

2，3，5-氯化三苯基四氮唑对N80^#钢缓蚀行为的研究

用稳态极化法和交流阻抗法研究了2，3，5-氯化三苯基四氮唑对N80^#钢在含H2SO3酸性溶液中发生腐蚀时的缓蚀作用及其缓蚀机理．结果表明：2，3，5-氯化三苯基四氮唑是一种以抑制阳极过程为主的缓蚀剂．其还原产物在N80^#钢表面的吸附阻止了腐蚀性的酸性溶液与钢表面的接触，从而起到缓蚀作用．