The dopaminergic innervation as observed by immunohistochemistry using anti-dopamine serum in the rat cerebral cortex

Summary By using an antiserum raised against dopamine bound to bovine serum albumin, thinner dopamine-labeled nerve terminals were visualized immunohistochemically within neocortical areas, in addition to well-documented dopaminergic innervation into the prefrontal and limbic cortices.This study was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas, Japanese Ministry of Education, Science, Culture (No. 62623002).     

治疗脑血栓药物效应的实验研究

脑血栓是脑血管栓塞或脑缺血性疾病。研究制疗脑血拴的药物,对脑血栓患者是一福音。本实验是用新研制的溶栓丸浸出液,做药物效应的动物实验报道。实验包括体外溶解血凝块的实验和体内溶解栓塞的实验。体内实验采取颈总动脉和颈外静脉插管搭桥手术法,人为的制成血管内栓塞的动物模型,用来研究药物的溶栓效应。实验结果证明,溶栓丸具有较强的溶栓效力。本项实验为溶栓丸治疗脑梗塞和脑血栓,提供了理论和实验根据。     

高级神经活动特征与灵敏协调反应的关系及应用

通过对运动员高级神经活动特征和灵敏协调反应的实验分析,得出了它们之间的正相关关系,并应用于飞行学员的飞行训练选材。对诸类与灵敏协调性有关的专业人员的选材提供了理论依据。     

大脑优势与第二语言习得

本文回顾了二十多年来神经语言学领域的重要研究,结合第二语言习得的有关研究,得出了与“临界期”假说完全不同的看法:成人与儿童(五岁以上)在第二语言习得过程中大脑神经因素没有区别性差异。     

Tauroursodeoxycholic acid prevents E22Q Alzheimer’s Aβ toxicity in human cerebral endothelial cells

The vasculotropic E22Q mutant of the amyloid-β (Aβ) peptide is associated with hereditary cerebral hemorrhage with amyloidosis Dutch type. The cellular mechanism(s) of toxicity and nature of the AβE22Q toxic assemblies are not completely understood. Comparative assessment of structural parameters and cell death mechanisms elicited in primary human cerebral endothelial cells by AβE22Q and wild-type Aβ revealed that only AβE22Q triggered the Bax mitochondrial pathway of apoptosis. AβE22Q neither matched the fast oligomerization kinetics of Aβ42 nor reached its predominant β-sheet structure, achieving a modest degree of oligomerization with a secondary structure that remained a mixture of β and random conformations. The endogenous molecule tauroursodeoxycholic acid (TUDCA) was a strong modulator of AβE22Q-triggered apoptosis but did not significantly change the secondary structures and fibrillogenic propensities of Aβ peptides. These data dissociate the pro-apoptotic properties of Aβ peptides from their distinct mechanisms of aggregation/fibrillization in vitro, providing new perspectives for modulation of amyloid toxicity. Received 20 November 2008; received after revision 12 December 2008; accepted 12 January 2009     

脑出血患者的护理体会

目的：探讨有效护理措施在脑出血预后中的作用．方法：总结37例脑出血患者的护理经验．结果：37例患者，根据脑出血日常生理神经预后标准评分（ADL），Ⅰ级4例，Ⅱ级15例，Ⅲ级14例，Ⅳ级2例，Ⅴ级1例，死亡1例．结论：脑出血治疗过程中的有效护理，能够明显减少并发症的发生，促进患者的康复，从而减少了死亡率和致残率．     

金玉教授妇科血症论治体验

对金玉导师在临床实践中治疗妇科血症方面经验进行总结.妇科血症可谓之常见的、多发的病症.为了探索妇科血症论治的基本方法、规律,从血的特性功效、病理入手,结合临床经验,辨证论治,归纳为＂血以调补＂的原则,运用活血化瘀、破瘀散结、养血活血、清热凉血、养津化燥、温经散寒、益气养血、滋精养血等方法.     

社会福利机构内小儿脑瘫康复模式探析

通过对目前脑瘫研究文献的梳理和康复实践的理论归纳,提出了基于不同康复环境及其资源下的家庭、医院及社会福利机构三种基本的脑瘫康复模式。在此基础上,探讨了社会福利机构康复模式在康复对象、康复手段和病因探索方面的特征,及对康复成本核算的方法及意义。     

胞二磷胆碱对大鼠脑缺血损伤后认知功能及海马神经元BCL-2,BAX表达和细胞凋亡的影响

探讨胞二磷胆碱对脑缺血损伤后大鼠认知功能和海马神经元BCL-2,BAX表达的影响.取健康Spra-gue-Dawley成年雄性大鼠60只,随机分为假手术对照组,缺血组和胞二磷胆碱组3组,每组20只.对缺血组和胞二磷胆碱组采用Zea Longa等法改良复制大脑中动脉狭窄(middle cerebral artery occlusion,MCAO)动物模型,通过Morris水迷宫系统观察各组大鼠学习记忆功能;免疫组化检测BCL-2,BAX表达,Tunel法检测神经元凋亡.胞二磷胆碱使Bcl-2蛋白表达从缺血对照组的39.88±5.41增加至55.13±8.17,BAX蛋白表达从缺血对照组的62.38±8.47下降至36.13±5.94,凋亡神经元从缺血对照组的18.67±3.86下降至14.67±4.25.胞二磷胆碱对脑缺血损伤后大鼠的神经保护作用可能是通过上调海马神经元Bcl-2蛋白、下调Bax蛋白的表达,减少海马神经元凋亡进而改善脑缺血损伤后大鼠的学习记忆能力.