乳腺癌PKCs表达与其分化程度的相关性

为了研究蛋白激酶C（PKC）和乳腺癌分化及转移的相关性，采用免疫组化SABC方法检测48例人乳腺癌的PKC蛋白表达，包括PKCα、βI、η和ξ等蛋白。结果表明，与分化低的乳腺癌对比，分化高的乳腺癌PKC蛋白表达水平提高。在临床I－II和III级的乳腺癌中，PKCη蛋白表达率分别为61．5％和9．1％，具有显著意义（P＜0．05），PKCξ蛋白表达率分别为92．3％和27．3％，具有极显著意义（P＜0．01）。另外，在乳腺癌未转移的病例中，PKCs蛋白表达水平普遍较高，其中PKCξ与肿转移相关性极显著（P＜0．01）。此外，PKCs蛋白表达水平与肿瘤患者年龄无关。以上结果提示，PKCη和PKCξ有可能作为评价临床乳腺癌分化和转移程度的指标之一。     

Laminin五肽对胃癌细胞系MGc－803的体外抑制作用

描述了Ｌａｍｉｎｉｎ五肽（ＹＩＧＳＲ）对胃癌细胞系ＭＧｃ－８０３的体外抑制作用，在多肽浓度为１ｍｇ／ｍｌ时抑制率高达７３％．抑制作用的机理可能是Ｌａｍｉｎｉｎ五肽竞争了肿瘤细胞上Ｌａｍｉｎｉｎ受体，从而封闭了肿瘤细胞对基底膜的结合及增殖．因此，利用Ｌａｍｉｎｉｎ五肽一类的药物有可能为肿瘤术后防止扩散提供一个新方法．     

Fecundity of the blood-feeding insectRhodnius prolixus increases in successive periods of egg production

The fecundity of the blood-feeding insect,Rhodnius prolixus, was observed to increase in successive periods of egg production, each period being triggered by a single large blood meal. As previously published, the fecundity of mated animals was significantly higher than that of unmated animals for the first period of egg production. For a second period of egg production, fecundity increased significantly in both mated and unmated animals. By the fourth period, fecundity had returned to first-feed values for mated animals, but remained high for unmated animals, and the fecundity of mated and unmated animals was not significantly different. Thus, during successive periods of egg production, the processes which maintain fecundity of unmated animals below that of mated animals are overcome.     

管流与环缝入流的管内合流特性──等温浓度场与紊流特性实验研究

在对管流与环缝入流的管内合流特性完成等温速度场实验研究的基础上，以对应一致的实验条件，实测了时均浓度分布，紊流正应力和紊流切应力分布．结果证明：浓度分布有与速度分布相一致的变化趋势和规律，但合流后截面上浓度均匀化比速度均匀化慢；合流动量相交产生附加雷诺应力，并决定合流后速度分布和浓度分布的均匀化进程．为建立系统的化学反应流动规律提供基础．     

Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2

Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4-phorbol 12-myristate, 13-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4-phorbol 12, 13 didecanoate was ineffective.These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.     

Effect of protein kinase C activation and depletion on insulin stimulation of glycogen synthesis in cultured hepatoma cells

Summary Insulin stimulation of glycogen synthesis was nearly abolished in hepatoma cells shortly treated with 4 ß-phorbol 12 \-myristate, 13 -acetate (protein kinase C activation) but remained unmodified in cells chronically treated with the phorbol ester (protein kinase C depletion). Thus, although exogenous activation of protein kinase C results in an inhibition of insulin action, protein kinase C depletion has no influence on this process. The results suggest that, in hepatoma cells, no endogenous activation of protein kinase C may occur in response to the signal triggered by insulin.     

In vitro protein synthesis and α amylase activity in F cells from hepatopancreas ofPalaemon serratus (Crustacea; Decapoda)

In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with³H-leucine, incorporation was highest in the F cell fractions. Measurements of -amylase activity, indicated that the two populations of F cells may be derived from the same cell type.     

实现净产值指标刍议

本文主要阐述了生产净产值指标在评价企业经济效益时的弊病,并从理论上和实践上论述了在商品经济条件下采用实现净产值指标评价企业经济效益的重要性和必要性。同时介绍了实现净产值指标的两种计算方法。即:生产法、分配法。     

中国代木包装的现状及发展研究(续)

4 今后发展代木包装的思考及研究我国代木包装虽兴起于七十年代,但在八十年代中期才受到应有的重视和较快的发展,现在在数量、品种、性能上还不能满足各方面包装的需要。随着今后国民经济的进一步发展,必然要求代木包装在数量、质量上要有一个更大的发展。为此,本文在前述对国内外代木包装分析的基础上,兹就今后发展代木包装的目标及方针,任务及规划,对策及措施初步探讨如下。