基于位点特异性打分矩阵的卷积神经网络预测SARS-CoV-2核衣壳蛋白的蛋白质二级结构

新型冠状病毒(SARS-CoV-2)有4种关键的结构蛋白,而核衣壳蛋白就是其中的1种.本实验从公开数据库NCBI上选取的SARS-CoV-2核衣壳蛋白质序列数据,分析SARS-CoV-2核衣壳蛋白与SARS-CoV核衣壳蛋白的序列相似性,对SARS-CoV-2核衣壳蛋白的理化性质和疏水性进行分析;在此基础上提出基于位点特异性打分矩阵的卷积神经网络,预测SARS-CoV-2核衣壳蛋白的8类蛋白质二级结构.研究结果表明,核衣壳蛋白的二级结构主要为无规卷曲,此结果可为抗病毒药物的研发与新型冠状病毒肺炎的诊断提供参考.     

基于LQG控制的整车8-DOF系统振动分析

建立了整车8-DOF系统动力学模型,考虑了主动悬架控制,并增设了主动座椅控制,设计了车辆主动悬架系统的LQG控制器。基于Matlab仿真平台建立了整车8-DOF系统动力学仿真模型,对所得最优控制策略下的动态响应进行了仿真验证。仿真结果表明:为了改善人椅系统质心及车身质心的跳振性能需要在一定程度上弱化各轮轮胎动位移性能。从控制效能上来看,该最优控制器能够满足各行驶状态下对悬架性能的要求,改善了车辆的行驶平顺性。     

酸性硅溶胶沉淀法制备超微细活性白炭黑

对酸性硅溶胶沉淀法生产超微细活性白炭黑的工艺进行了研究；探讨了生产过程中硅溶胶的ｐＨ值，反应终点ｐＨ值，反应温度以及分散剂等条件；制备了较高品质的产品，其性能接近或超过国外同类产品．     

Modulation of protein biophysical properties by chemical glycosylation: biochemical insights and biomedical implications

Glycosylation constitutes one of the most important posttranslational modifications employed by biological systems to modulate protein biophysical properties. Due to the direct biochemical and biomedical implications of achieving control over protein stability and function by chemical means, there has been great interest in recent years towards the development of chemical strategies for protein glycosylation. Since current knowledge about glycoprotein biophysics has been mainly derived from the study of naturally glycosylated proteins, chemical glycosylation provides novel insights into its mechanistic understanding by affording control over glycosylation parameters. This review presents a survey of the effects that natural and chemical glycosylation have on the fundamental biophysical properties of proteins (structure, dynamics, stability, and function). This is complemented by a mechanistic discussion of how glycans achieve such effects and discussion of the implications of employing chemical glycosylation as a tool to exert control over protein biophysical properties within biochemical and biomedical applications. Received 15 December 2006; received after revision 28 March 2007; accepted 25 April 2007     

Regulation of insulin receptor function

Resistance to the biological actions of insulin contributes to the development of type 2 diabetes and risk of cardiovascular disease. A reduced biological response to insulin by tissues results from an impairment in the cascade of phosphorylation events within cells that regulate the activity of enzymes comprising the insulin signaling pathway. In most models of insulin resistance, there is evidence that this decrement in insulin signaling begins with either the activation or substrate kinase activity of the insulin receptor (IR), which is the only component of the pathway that is unique to insulin action. Activation of the IR can be impaired by post-translational modifications of the protein involving serine phosphorylation, or by binding to inhibiting proteins such as PC-1 or members of the SOCS or Grb protein families. The impact of these processes on the conformational changes and phosphorylation events required for full signaling activity, as well as the role of these mechanisms in human disease, is reviewed in this article. Received 3 August 2006; received after revision 1 December 2006; accepted 8 January 2007     

Cellular internalization of proinsulin C-peptide

Proinsulin C-peptide is known to bind specifically to cell membranes and to exert intracellular effects, but whether it is internalized in target cells is unknown. In this study, using confocal microscopy and immunostained or rhodamine-labeled peptide, we show that C-peptide is internalized and localized to the cytosol of Swiss 3T3 and HEK-293 cells. In addition, transport into nuclei was found using the labeled peptide. The internalization was followed at 37°C for up to 1 h, and was reduced at 4°C and after preincubation with pertussis toxin. Hence, it is concluded to occur via an energy-dependent, pertussis toxin-sensitive mechanism and without detectable degradation within the experimental time course. Surface plasmon resonance measurements demonstrated binding of HEK-293 cell extract components to C-peptide, and subsequent elution of bound material revealed the components to be intracellular proteins. The identification of C-peptide cellular internalization, intracellular binding proteins, absence of rapid subsequent C-peptide degradation and apparent nuclear internalization support a maintained activity similar to that of an intracrine peptide hormone. Hence, the data suggest the possibility of one further C-peptide site of action. Received 31 October 2006; received after revision 27 December 2006; accepted 30 December 2006     

一类特殊的有限群

研究了有限群部分元素乘积的问题,讨论了在有限群中存在的一类特殊群—超P-群,证明了所有的有限循环群是超P-群,并给出了一个21阶的有限非Abelian群是超P-群.     

用N-(3-P)NEM标记毛发角蛋白研究毛发中低硫蛋白和高硫蛋白的状态

本文用一种特异性标记蛋白质巯基的N-(3-P)NEM为荧光探针标记毛发角蛋白,经SDS-PAGE后,通过荧光薄层扫描确定N-(3-P)NEM与含巯基蛋白结合的部位。结果表明,毛发角蛋白中的低硫蛋白分子量在45,000～63,000范围,约占总蛋白量的49.6％～68.2％;高硫蛋白分子量在4,000～20,000范围,约占总蛋白量的18.9％～36.2％。除低硫和高硫蛋白以外,毛发角蛋白中还可能存在一种极低硫蛋白,分子量位于22,000～32,000范围。对人与5种动物的毛发角蛋白组分进行比较,证实人与动物的毛发角蛋白的主要差异在高硫蛋白,不同种属的动物毛发角蛋白中低硫蛋白和高硫蛋白的含量也不相同。作者认为毛发角蛋白中含巯基蛋白的差异可为动物分类学和法医学在进行毛发比较鉴别方面提供新的重要信息。