某鸡场鸡传染性法氏囊病弱毒疫苗饮水免疫后发生的一次鸡病的诊断

１９９１年４月上旬，四川某鸡场对３５７０只１３日龄依莎雏鸡作法氏囊病弱毒疫苗饮水免疫，１４日龄时发病死亡９只，到２３日龄９天内共死亡８１４只，病死率达２２．８％。对２０只送检病死鸡的沙门氏菌分离率为１／２０株（５％）．但病料人工感染小白鼠试验为阴性，证明非病原菌所致，用病料与新城疫血清（抑制价１：６４０）作用后接种非免疫健康鸡１组的存活率为７５％，直接接种未与新城疫血清作用病料的２组存活率为５０％，剖检回归本动物试验自然死亡和人为处死的病鸡发现了较为典型的法氏囊病变，由此认为：法氏囊弱毒疫苗饮水免疫是引起鸡群发病死亡的主要原因。     

人乳头瘤病毒感染的宫颈癌组织的病理形态学观察

应用核酸杂交技术结合组织病理学的光镜和电镜观察、检测８０例子宫颈癌、４２例正常宫颈组织、３３例宫颈非典型增生组织和１０例宫颈尖锐湿疣组织中的ＨＰＶｓ基因组相关序列。结果表明：（１）４２例正常宫颈组织ＤＮＡ无一例检出ＨＰＶ，而在宫颈癌组织、宫颈非典型增生组织和宫颈尖锐湿疣组织中，ＨＰＶ检出率分别达４３．７５％、４５．４５％和８０％；（２）宫颈癌组织中阳性３５例，阳性率为４３．７５％；ＨＰＶ型别分布主要为ＨＰＶ１６型（１８例，５１．４３％）和ＨＰＶＲ型（８例，２２．８６％）；（３）１８例ＨＰＶ１６型和３例ＨＰＶ１１／６型均为鳞癌，８例ＨＰＶＲ型中有６例也为鳞癌。ＨＰＶ１６型的１８例中有１２例为低分化癌，ＨＰＶＲ型的８例中有５例也为低分化癌；（４）不同型别ＨＰＶ感染的宫颈癌组织，其病理形态学特征有所不同。研究表明，广西地区相当部分宫颈癌的发生与ＨＰＶ感染有关，其感染的宫颈癌组织具有一定的病理形态学特征。     

竹丛枝病的研究——Ⅲ.病菌的侵染特点和防治试验

竹丛枝病菌(Balansia talce)在病枝内越冬后,翌春在病枝新梢上产生分生。孢子成为初次侵染来源。带病竹母造林经连续多年观察,病、健株鞭系剖析调查、自然发病和人工接种发病新梢,以及剪除病枝连续多年观察结果,认为该病是局部侵染性病害。彻底剪除病枝(株)即不再复发;粉锈宁对症状有一定的抑制作用,四环素等抗生素对症状无防治作用。     

外源性生长激素对改善腹腔感染时生长激素不敏感状态中的作用

探索了外源性生长激素对改善腹腔感染时生长激素不敏感中的作用。应用盲肠结扎穿孔法（CLP）制备腹腔感染大鼠模型,同时给予外源性生长激素;应用放射免疫法测定血清生长激素（GH）水平;应用逆转录多聚酶链反应（RT-PCR）法测定肝组织胰岛素样生长因子I(IGF-I)、生长激素受体（GHR）和细胞因子信号传导抑制体3(SOCS-3)mRNA的表达;应用ELISA测定血清肿瘤坏死因子α(TNF-α)和白介素6(IL-6)水平。结果表明腹腔感染组大鼠血清生长激素水平与对照组无明显差异,但肝组织IGF-ImRNA表达明显下降;给予外源性生长激素后可明显降低血清TNF-α和IL-6水平,同时可更快地促使GHR和IGF-ImRNA表达的上调及下调SOCS-3mRNA的表达。腹腔感染时机体存在对生长激素的不敏感状态,给予外源性生长激素后可明显地改善感染状态下机体对生长激素的敏感性。     

医院内病原菌及药敏谱信息处理系统

本文介绍了医院内病原菌的流行及药敏谱信息处理软件系统的结构、设计思想、运行环境及功能等.它将病原菌及其耐药情况,抗生素的合理使用,院内感染监察及药事管理诸项工作有机地联为一体,可随时向有关方面提供大量的纵横统计分析数据报表(共13类)和直观的图形(两百多个直方图和5类 Ridit 分析图).     

宜春地区肺吸虫第二中间宿主：—溪蟹感染情况的调查

自1982年来,全区共采集溪蟹3208只,定性解剖1684只,阳性1258只,感染率为74.70%,其中1只雄性蟹体内检获囊蚴6599只.部体解剖,91.47%囊蚴分布在肌肉、蟹足、螯肢.用新鲜囊蚴人工感染家犬获得成功.其囊蚴、虫卵、成虫均为卫氏并殖型.     

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process.Received 25 November 2003; received after revision 8 January 2004; accepted 21 January 2004     

普通级啮齿类实验动物部分细菌携带情况的初步调查

了解在实际生产中常用普通级啮齿类实验动物中清洁级规定排除的4种主要细菌(沙门菌、鼠棒状杆菌、嗜肺巴斯德杆菌、支气管败血性鲍特菌)的携带状况,以及验证筛选的实验动物细菌检测常用普通培养基和生化培养基的应用效果.方法无菌采集动物气管分泌物,接种血平皿;采集回盲部内容物接种DHL、SS琼脂平皿,37℃48h后挑取可疑菌落进行纯化,取纯培养物进行染色镜检、生化特性鉴定及血清学反应.结论KM小鼠嗜肺巴斯德杆菌阳性率最高,为60%;豚鼠携带支气管败血性鲍特菌,阳性率为30%;总的细菌检出率为20%(其中嗜肺巴斯德杆菌总阳性率为16.25%,支气管败血性鲍特菌总阳性率为3.75%);选出的培养基应用于实验检测中是可行的.     

Structure and morphogenesis of bacteriophage T4

Bacteriophage T4 is one of the most complex viruses. More than 40 different proteins form the mature virion, which consists of a protein shell encapsidating a 172-kbp double-stranded genomic DNA, a tail, and fibers, attached to the distal end of the tail. The fibers and the tail carry the host cell recognition sensors and are required for attachment of the phage to the cell surface. The tail also serves as a channel for delivery of the phage DNA from the head into the host cell cytoplasm. The tail is attached to the unique portal vertex of the head through which the phage DNA is packaged during head assembly. Similar to other phages, and also herpes viruses, the unique vertex is occupied by a dodecameric portal protein, which is involved in DNA packaging.Received 18 February 2003; received after revision 16 April 2003; accepted 9 May 2003