新指示反应催化光度法测定痕量铁的研究

研究了在溴化十六烷基吡啶存在下，以邻菲啉为活化剂，铁催化抗坏血酸还原对羟基苯基重氮氨基偶氯苯的褪色反应及其动力学条件，探讨了反应机理，建立了高选择性测定痕量铁的新方法．本法检出限为９５×１０－１１ｇ／ｍｌ．线性范围为０－１０μｇ／２５ｍｌ．用于发样、食品样中痕量铁的测定，结果满意．     

乳腺癌PKCs表达与其分化程度的相关性

为了研究蛋白激酶C（PKC）和乳腺癌分化及转移的相关性，采用免疫组化SABC方法检测48例人乳腺癌的PKC蛋白表达，包括PKCα、βI、η和ξ等蛋白。结果表明，与分化低的乳腺癌对比，分化高的乳腺癌PKC蛋白表达水平提高。在临床I－II和III级的乳腺癌中，PKCη蛋白表达率分别为61．5％和9．1％，具有显著意义（P＜0．05），PKCξ蛋白表达率分别为92．3％和27．3％，具有极显著意义（P＜0．01）。另外，在乳腺癌未转移的病例中，PKCs蛋白表达水平普遍较高，其中PKCξ与肿转移相关性极显著（P＜0．01）。此外，PKCs蛋白表达水平与肿瘤患者年龄无关。以上结果提示，PKCη和PKCξ有可能作为评价临床乳腺癌分化和转移程度的指标之一。     

超微粒子－有机聚合物薄膜的研究

我们用离子团束－飞行时间质谱（ＩＣＢ－ＴｏＦＭＳ）系统制备了金（Ａｕ）超微粒子－聚乙烯（ＰＥ）薄膜和Ｃ＿（６０）－聚乙烯薄膜。用透射电子显微镜（ＴＥＭ）分析了所制备的样品。它们的结构是Ａｕ超徽粒子镶嵌在多晶的ＰＥ薄膜中，其中Ａｕ原子团呈球形，直径分布在２．０－５．０ｎｍ窄范围内，当沉积基底温度为９０℃时，Ａｕ原子团相互靠近，几乎连接起来，但仍保持原来大小。基底温度为１４０℃蒸积的Ｃ＿（６０）－ｐＥ薄膜具有晶态结构，其晶格常数为１．４５４ｎｍ。     

逻辑学语境下的科学实用主义

作为实用主义的鼻祖,皮尔士首先和主要是一位逻辑学家.他的实用主义从根本上讲是一种逻辑学说,要求从逻辑学理论上对其进行解读.审视科学实用主义同逻辑学的内在的、历史的联系及其形而上的蕴含,可以更清晰地认识实用主义理论的本真意义.     

基于YT0-101总线的PC与单片机通信系统的研究

在对YT0—101通信接口电路及其通信协议研究的基础上，探讨了PC机与8051单片机之间进行etech—bus串口通信软件和硬件设计的原理及方法，给出了单片机与YTO—101总线的部分接口程序。     

Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2

Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4-phorbol 12-myristate, 13-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4-phorbol 12, 13 didecanoate was ineffective.These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.     

Effect of protein kinase C activation and depletion on insulin stimulation of glycogen synthesis in cultured hepatoma cells

Summary Insulin stimulation of glycogen synthesis was nearly abolished in hepatoma cells shortly treated with 4 ß-phorbol 12 \-myristate, 13 -acetate (protein kinase C activation) but remained unmodified in cells chronically treated with the phorbol ester (protein kinase C depletion). Thus, although exogenous activation of protein kinase C results in an inhibition of insulin action, protein kinase C depletion has no influence on this process. The results suggest that, in hepatoma cells, no endogenous activation of protein kinase C may occur in response to the signal triggered by insulin.     

一种防空C~3I系统的效能分析方法

本文讨论了一种评定C~3I系统效能的方法。这种方法是根据系统完成使命的能力和系统的内部结构两方面的分析来完成的。通过对IFFN系统的评定,对本方法进行了举例说明。     

Feller—Trotter算子的局部饱和定理

利用Bajsanski-Bajanic抛物线引理建立Feller-Trotter算子的局部小“ο”饱和定理,进一步地应用所得到结论建立了(C_)类算子半群概率表示的局部饱和定理。     

Proopiomelanocortin expression in the skin during induced hair growth in mice

We demonstrate for the first time a hair cycle-dependent gene and protein expression of proopiomelanocortin in mouse skin in vivo. Northern blot detected POMC mRNA with an apparent size of 0.9 kb in anagen but not telogen skin. Western blot emphasized a specific protein of 30–33 kDa recognized by anti -endorphin in late but not early anagen or telogen skin. By immunocytochemistry, -endorphin antigen was localized in the sebaceous gland in a hair cycle dependent manner.