排序方式: 共有36条查询结果,搜索用时 15 毫秒
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用部分酶切法切开表达质粒 pMZR 中一个 HindⅢ位点后,插入了一个带 HindⅢ粘性末端的1kb 硅藻 DNA 片段重组 DNA 分子转化 E.coli LE392,对转化子中的重组 DNA 分子用酶切分析后,鉴定了1kb DNA 片段插入 pMZR 的位置。 相似文献
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马宏 《河北省科学院学报》2006,23(3):8-10
将能以葡萄糖为唯一碳源积累P(3HB)的工程菌进行优化筛选,获得6个较高产的菌株,PHB含量最高为69.1%.经丙酸驯化,虽然没有PHBV的积累,但PHB的量均有增加,最高可达71.13%.另外,抗生素对菌体生长及PHB积累有重要影响. 相似文献
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C. Gordon-Thomson A. Kumari L. Tomkins P. Holford J. T. Djordjevic L. C. Wright T. C. Sorrell G. P. M. Moore 《Cellular and molecular life sciences : CMLS》2009,66(6):1116-1125
Chitotriosidase secreted by activated human macrophages has been implicated in the defence against chitin-bearing pathogens.
The antifungal properties of human chitotriosidase were investigated here following retroviral vector-mediated gene transfer
of the open reading frame of the chitotriosidase gene into Chinese hamster ovary cells. A chitinase assay confirmed that the
engineered cells secreted recombinant chitotriosidase constitutively. Two dimensional gel electrophoresis and western blotting
indicated that the recombinant protein is the major, chitin-binding, fifty kilodalton isoform. Culture medium conditioned
by the transduced cells inhibited growth of isolates of Aspergillus niger, Candida albicans and Cryptococcus neoformans. Furthermore, longevity was significantly increased in a mouse model of cryptococcosis when cells transduced with the chitotriosidase
gene and encapsulated in alginate microspheres were implanted subcutaneously in the animals. Engraftment of microcapsules
containing cells transduced with the chitotriosidase gene has the potential to combat infections caused by chitinous pathogens
through the prolonged delivery of recombinant chitotriosidase.
Received 29 November 2008; received after revision 11 January 2009; accepted 13 January 2009 相似文献
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重组DNA技术于1972年诞生于美国高校实验室。重组DNA咨询委员会(简称RAC)问世于1974年,并于2019年退出历史舞台,为美国基因操作技术的风险规制立下了汗马功劳。RAC在美国基因操作技术风险规制中跌宕起伏的演变史,是美国基因操作技术的风险规制由稚嫩逐步走向成熟的印证。通过探究RAC及其监管权是在哪些力量的作用下问世,还原RAC在基因操作技术风险阴霾笼罩下被政府当局质疑能力的历史图景,呈现美国基因治疗的基本规制路径,描述RAC审批首次基因治疗的经过以及RAC在首次基因治疗致死事件中的应对表现,阐述RAC退出历史舞台的过程和缘由,从而描摹一幅美国重组DNA咨询委员会演变历程的脉络图。 相似文献