Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S5n allel...     

紫荆的受精作用与胚,胚乳发育的研究

应用光学显微镜对紫荆的受精作用与胚乳、胚胎的形成进行了初步观察。珠孔受精,精子与极核的融合先于与卵融合,雌、雄性核的融合方式为有丝分裂前配子融合的类型,核型胚,初生胚,核先于合子分裂,合子第一次分裂为横裂,胚胎发育属于Souèges的A_1型,相当于柳叶莱型。     

龟足（Pollicipes mitella）的胚胎发育

本文详细描述了龟足（Ｐｏｌｌｉｃｉｐｅｓｍｉｔｅｌｌａｌｉｎｎａｅｕｓ１７５８）从受精卵至孵化成无节幼虫的发育过程。卵细胞的两次成熟分裂是在受精后才先后完成，卵裂为螺旋形卵裂，若水温为２９～３１℃，从受精至孵化约需８～１５天。     

暗纹东方鲀胚胎发育的观察

详细记叙了暗纹东方　胚胎发育各阶段的形态特征及早期发育的生物学资料．暗纹东方　的卵为沉性卵，入水后具粘性，其粘度与受精效果有关．卵径为１．１１８～１．２７４ｍｍ，具油球２８０～３９０个，油球径为０．０２６～０．０７８ｍｍ．作者采用连续法对受精卵的发育进行观察．在水温１８．７～２１．２℃时，受精卵历时１３９ｈ１０ｍｉｎ开始孵出仔鱼．其发育过程划分为３５个时期．本研究可为开展暗纹东方　的人工育苗提供必要的参考资料．     

人心果大,小孢子,胚及胚乳的发育和结构研究

本文主要报道人心果（Ｍａｎｉｌｋａｒａｚａｐｏｔａ）大、小孢子，胚和胚乳的发育和结构。研究结果如下：１、胚囊的发育属蓼型；胚乳的发育属核型；小孢子的发育属连续型。２、胚珠倒生，具单层珠被．３、４分体的细胞发育是不同步的。４、成熟的花药约有１／４－１／３花粉粒是败育、干瘪的。     

Effect of matrix metallo-proteinase-26 (MMP-26) during embryo implantation in the mouse

Matrix metalloproteinase-26 (MMP-26, endometase and matrilysin-2), a novel member of the MMPs family, is detected not only in the placenta and uterus, but is widely expressed in malignant tumors from different sources as well as in diverse tumor cell lines. However, the function of MMP-26 in the reproductive system has never been reported. Expression of MMP-26 in mouse embryos and the function of the MMP-26 antibody during mouse embryo implantation was examined for the first time by injecting the uterine horn, immunohistochemistry,in situ hybridization, co-culture of mouse blastocysts and uterine monolayer epithelial cells, Western blot, RT-PCR, Northern blot and zymography. Our results show that there is strong expression of MMP-26 mRNA and protein in the mouse embryo. Furthermore, the MMP-26 antibody dramatically inhibited mouse embryo implantation and significantly inhibited adhesion and outgrowth of mouse blastocysts onin vitro uterine monolayer epithelial cells. At the same time, the MMP-26 antibody inhibited the expression of integrin αV mRNA and protein in a dose-dependent manner. These data suggest that MMP-26 may play a role in some of the tissue-remodeling events associated with the invasion of the endometrium by trophoblast cells and facilitate successfully embryo implantation.     

影响体细胞核移植重构胚发育的因素

目前已有多种动物被成功克隆,但存在着诸多未知因素,使体细胞核移植重构胚发育至囊胚阶段的比例过低,克隆动物存在早衰等异常现象.该文围绕这产生这些现象的根本原因、高度分化的体细胞核移入卵质后所发生的分子事件以及其影响因素如体细胞的来源和培养代数、细胞周期、核质相互作用、细胞核再程序化、线粒体等方面对体细胞核移植重构胚发育的影响等进行综述.     

巨峰葡萄胚培养技术的研究

选用巨峰葡萄在花后35～60 d,取其胚接种在含有GA3I、AA、6-BA的不同激素水平MS培养基上进行胚挽救,以研究葡萄的幼胚培养技术.结果表明,巨峰葡萄在花后60 d效果最好.在附加GA30.5 mg/LI、AA1.0 mg/L、6-BA0.5 mg/L的最佳培养基上得到了(76.0±16.7)%的发育率和(69.1±13.1)%的成苗率.     

哺乳动物胚胎冷冻技术研究进展

报道了胚胎冷冻技术的发展及现状。根据它的发展分别介绍了慢冻慢解法、常规快速解冻法、玻璃化冷冻、1步细管法冷冻、1步冷冻和半胚冷冻，并且就胚胎冷冻保护剂、冷冻方法、解冷方法、冷冻保护剂的脱除及胚胎发育时期等对胚胎冷冻效果的影响进行了探讨。     

温敏无核荔枝的种子败育和果实发育研究

运用胚胎学、解剖学的方法 ,对海南新发现的温敏无核荔枝产生无核果实的原因进行研究 .结果表明 :温敏无核荔枝由于低温影响 ,在受精过程中 ,精子不能进入卵膜或进入卵后雌雄核不融合 ,导致卵受精失败并退化 .次生核虽正常受精 ,并产生核型胚乳 ,有的还能发育到细胞型胚 ,到 12d左右也完全退化 .结果导致胚珠极早败育 ,但假种皮仍能发生发育 ,最终形成无核果实