| 巨峰葡萄胚培养技术的研究 |
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| 引用本文： | 尚忠海.巨峰葡萄胚培养技术的研究[J].河南科学,2007,25(3):405-408. |
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| 作者姓名： | 尚忠海 |
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| 作者单位： | 河南农业大学,林学园艺学院,郑州,450002;河南省林业技术推广站,郑州,450008 |
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| 摘 要： | 选用巨峰葡萄在花后35～60 d,取其胚接种在含有GA3I、AA、6-BA的不同激素水平MS培养基上进行胚挽救,以研究葡萄的幼胚培养技术.结果表明,巨峰葡萄在花后60 d效果最好.在附加GA30.5 mg/LI、AA1.0 mg/L、6-BA0.5 mg/L的最佳培养基上得到了(76.0±16.7)%的发育率和(69.1±13.1)%的成苗率.
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| 关 键 词： | 胚培养 巨峰 发育率 成苗率 |
| 文章编号： | 1004-3918(2007)03-0405-04 |
| 修稿时间： | 2007-03-01 |
The Study on the Technique of Embryo Culture of 'Kyoho' Grape |
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| SHANG Zhong-hai.The Study on the Technique of Embryo Culture of ''''Kyoho'''' Grape[J].Henan Science,2007,25(3):405-408. |
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| Authors: | SHANG Zhong-hai |
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| Institution: | 1. College of Forestry and Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Provincial Forestry Extension Station, Zhengzhou 450008, China |
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| Abstract: | 35～60 days after florescence,embryos of 'Kyoho'(V.vinifera×V.labrusce cv.) grapes were cultured on MS medium supplemented with different concentrations of GA3,IAA and 6-BA in order to study the techniques of embryo culture.The results indicated that the best period of inoculation of'Kyoho'embryo was 60 days after florescence.We got(76.0±16.7) % development rate and(69.1±13.1) % germinate rate(p<0.01) of 'Kyoho' embryo in the medium with MS+GA3 0.5 mg / L. |
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| Keywords: | embryo culture Kyoho development rate germinate rate |
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