WDR34 is a novel TAK1-associated suppressor of the IL-1R/TLR3/TLR4-induced NF-κB activation pathway

Toll-like receptors (TLRs) act as sensors of microbial components and elicit innate immune responses. All TLR signaling pathways activate the nuclear factor-kappaB (NF-κB), which controls the expression of inflammatory cytokine genes. Transforming growth factor-β-activated kinase 1 (TAK1) is a serine/threonine protein kinase that is critically involved in the activation of NF-κB by tumor necrosis factor (TNFα), interleukin-1β (IL-1β) and TLR ligands. In this study, we identified a novel protein, WD40 domain repeat protein 34 (WDR34) as a TAK1-interacting protein in yeast two-hybrid screens. WDR34 interacted with TAK1, TAK1-binding protein 2 (TAB2), TAK1-binding protein 3 (TAB3) and tumor necrosis factor receptor-associated factor 6 (TRAF6) in overexpression and under physiological conditions. Overexpression of WDR34 inhibited IL-1β-, polyI:C- and lipopolysaccharide (LPS)-induced but not TNFα-induced NF-κB activation, whereas knockdown of WDR34 by a RNA-interference construct potentiated NF-κB activation by these ligands. Our findings suggest that WDR34 is a TAK1-associated inhibitor of the IL-1R/TLR3/TLR4-induced NF-κB activation pathway. D. Gao and R. Wang contributed equally to this work.     

人线粒体蛋白编码基因C2ORF47在细胞增殖与凋亡中的作用初探

线粒体在真核细胞中扮演着重要的角色,其功能障碍与许多疾病的发生密切相关.在这项研究中,我们鉴定了一个有Tim44样结构域(Tim44-like domain)的人类线粒体蛋白编码基因C2ORF47(chromosome 2openreading frame 47).该基因是从人的睾丸组织中克隆出来的,在人体各种组织中广泛表达.序列比对和进化分析表明,该蛋白质和其同系物在脊椎动物中是保守的.采用免疫荧光技术,我们发现该基因产物定位于线粒体.过表达C2ORF47可以抑制细胞增殖.使用siRNA敲减内源的C2ORF47能促进细胞增殖.此外,敲减C2ORF47会导致糖和血清饥饿引发的细胞凋亡大幅减少.总之,在细胞增殖和由饥饿引发的细胞凋亡的调控中,该基因可能发挥重要作用.     

水热法制备Co3O4及其超电容性能的研究

以硝酸钴为原料、尿素为沉淀剂、柠檬酸三纳为模板,用水热法制备了Co3O4,并采用循环伏安、恒流充放电等方法研究了样品的电化学性能.结果表明,Co3O4电极在6mol·L-1KOH溶液中、在0～0.4V电位范围内、电流密度为5mA·cm2时,单电级比电容可达900F·g-1.     

支持跨域协作的P2P网络管理系统设计与实现

为了解决大规模网络的可扩展性和跨域协作问题,提出了一种基于P2P技术的、支持跨域协作的网络管理体系结构。在该体系中,每个管理域独立存在且高度自治,每个管理域通过iManager和iAgent两种实体实现本域的管理功能并向外发布本域的管理服务;同时,多个管理域之间采用P2P方式进行松耦合的连接,利用ambassador实体通过域间通信协议搜索和调用其他域的管理服务。为了满足网络管理系统对服务搜索效率的需求,还实现了基于启发式条件的P2P服务搜索算法。该文设计和实现了相应的试验系统,试验系统已经用于下一代教育科研试验网CNGI-CERNET2主干网,运行良好。     

基于3S技术的第二次全国土地调查与分析

2008年第二次全国土地调查在全国范围内展开，这对于全面查清全国土地利用状况，掌握真实的土地基础数据具有极其重要的意义。3S技术是当前空间技术发展的重要方向，在第二次全国土地调查中发挥了重要的作用。通过重点论述土地调查的技术路线和方法，介绍内业建库的过程并且分析了调查数据变化的原因。     

一种基于HLA Evovled的云仿真技术研究

针对现有HLA仿真资源难以与Internet共享使用的问题,借鉴云计算的思想,采用HLA Evolved相关的技术改进,提出了一种基于HLA Evolved的云仿真的体系结构和框架实现方案;研究了用于Web服务的RTI组件、模块化FOM/SOM对象模型和WSDL API函数等HLA Evolved技术,解决了基于多层语义的服务注册、发现、组合和平台的监控、预测及基于Hadoop的分布式存储、仿真技术;完成了一种战术通信网系统的云仿真测试实验,证明了基于HLA Evolved的云仿真设计方法的可行、有效。     

四方晶系下钕离子的基态分裂

采用点电荷晶场模型,通过模拟四方晶系化合物NdCuAs2和Nd2Ge2In的磁化率倒数-温度曲线,得到了Nd3+的各级分裂能和相应波函数,并分析了晶场效应对化合物磁性质的影响.结果表明,Kramers离子Nd3+的十重简并基态4I9/2在晶场效应的作用下分裂为5个双态,计算结果与实验吻合得较好.     

关于Zeta函数值的一个注记

关于Zeta函数的特殊值，Browkin曾猜测有等式｜ζ（-1）｜=R2k2/ω2，然而，在F是有复素位的数域情形下，可判定此等式不成立。主要的方法是利用有关Bloch群的一个定理及zeta函数方程作一些计算，然后和猜测的等式进行比较，导出矛盾。     

LLDPE/TiO2 nanocomposites produced from different crystallite sizes of TiO2 via in situ polymerization

Nano-TiO2 particles with a range of crystallite sizes were synthesized by a conventional sol-gel method,and then used as nanoparticle substrates in the synthesis of LLDPE/TiO2 nanocomposites via in situ polymerization of ethylene/1-hexene with zirconocene/MMAO catalyst.It was found that the size of the nano-TiO2 crystallite nanoparticles can influence the catalytic activity in the polymerization system.The larger nano-TiO2 crystallites provided better catalytic activity in the polymerization system due to more space for monomer attack.In addition,by thermo-gravimetric analysis,it can be seen that the larger nano-TiO2 crystallites also exhibited lower interaction with available MMAO.Consequently,the MMAO reacted more efficiently with the zirconocene catalyst during the activation process,and enhanced polymerization catalysis.All the polymer nanocomposites products did not have well defined melting temperature indicating non-crystalline polymers.This is due to the high amount of hexene incorporation(based on 13C NMR).The difference in crystallite sizes of the nano-TiO2 also affected how 1-hexene became incorporated into the polymer nanocomposites.The smaller crystallite size of nano-TiO2 allowed greater 1-hexene incorporation due to depression of the reactivity of the ethylene.The contribution of this work helps develop a better understanding of the role of nano-TiO2 in the catalytic activity of the polymerization system and in the microstructure of the polymer composite product.However,this study only considers work on the laboratory scale,so for commercial application of these results,it is necessary to scale up the polymerization process.It is only at this stage,that other physical properties,such as the mechanical properties of these materials can be sensibly determined.     

基于{V_8O_(24)}~(8-)阴离子簇的一维Zn(Ⅱ)配合物的合成及晶体结构

利用水热合成技术,合成了1个基于{V8O24}8-阴离子簇的Zn(Ⅱ)配合物[Zn2(Hdpa)3]2V8O24(dpa=2,2′-dipyridylamine),通过X-射线单晶和粉末衍射、红外光谱和热重-差热分析手段进行了表征.单晶X-射线衍射分析表明化合物是由2种不同的阳离子片段([Zn(dpa)2]2+和[Zn(dpa)]2+)连接{V8O24}8-阴离子簇形成的1D带状结构,其中{V8O24}8-阴离子簇展示了清晰的椅式构型,2个[Zn(dpa)]2+单元分别被支撑在椅式构型的两侧.