半(p,r)-预不变凸函数及其规划的最优性条件

首先在半预不变凸函数和(p,r)-预不变凸函数的基础上,定义了一类新的广义凸函数半(p,r)-预不变凸函数,它既是半预不变凸函数又是(p,r)-预不变凸函数的推广形式,从而是熟知的凸函数和不变凸函数的推广形式.接着,讨论了半(p,r)-预不变凸函数的一些有用性质.最后利用半(p,r)-预不变凸(凹)函数讨论了目标函数和约束函数均不可微的多目标规划问题,从而获得两个最优性条件.     

清热利湿与益气古典方剂逆转二甲基亚硝胺大鼠肝硬化的细胞生物学基础研究

探讨了清热利湿的茵陈蒿汤与益气的黄芪汤逆转二甲基亚硝胺(di methylnitrosamine,DMN)大鼠肝硬化的细胞生物学基础.采用0.5%DMN生理盐水溶液4周12次腹腔注射法制备大鼠肝硬化模型,造模成型后终止DMN刺激,分组予以不同功效方剂经口给药2周,获取样本作相关指标的检测.TUNEL染色原位检测肝细胞凋亡并计算凋亡指数,免疫组织化学及Western blot方法分别检测肝组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、肝细胞生长因子α(he-patic growth factor-α,HGF-α)、CD68及基质金属蛋白酶13(MMP-13)的表达,明胶酶谱法检测基质金属蛋白酶2,9(MMP-2、MMP-9)的活性.与正常大鼠比较,模型大鼠肝细胞凋亡指数、CD68及α-SMA表达均显著增加,而HGF-α与MMP-13表达显著减少(P<0.01或<0.05);与同期模型对照组大鼠相比,茵陈蒿汤组大鼠肝细胞凋亡指数显著降低,CD68与MMP-13表达显著增加(P<0.01,P<0.05),黄芪汤组无显著变化;茵陈蒿汤组MMP-9活性显著增加(P<0.01)、而黄芪汤组显著降低(P<0.05);茵陈蒿汤组及黄芪汤组大鼠肝组织α-SMA表达均显著降低(P<0.01),而黄芪汤组显著低于茵陈蒿汤组(P<0.05),黄芪汤组大鼠肝组织HGF-α表达显著增加(P<0.05),MMP-2活性显著降低(P<0.05).这表明逆转DMN大鼠肝硬化的有效方剂具有不同的细胞生物学基础:茵陈蒿汤清热利湿的祛邪作用在于减少肝实质细胞的病理性凋亡、调控库普弗细胞功能;黄芪汤益气的扶正功效主要表现为抑制肝星状细胞活化及促进肝窦内皮细胞损伤的修复.     

Interaction of aloe-emodin with human serum albumin

The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased.     

基于DDS算法的新型多功能开关电源的研制

提出了一种三相交流36 V开关稳压电源的设计思路.此设计采用TOP系列单片开关电源芯片来代替传统的线性稳压芯片,利用高频变压器来代替传统变压器,极大的缩小设计体积;采用高性能8位单片机ATmega8模拟DDS算法来进行编程控制,从而产生交流36 V,400 Hz中频逆变稳压输出.     

子洲地区山西组及盒8段有利砂体成因探讨

目的为了查明鄂尔多斯盆地东部子洲地区山西组及盒8段有利砂体的特征及成因,进而寻找有利砂体及高产天然气聚集区。方法采用野外观察与室内研究相结合的方法。结果大量的砂岩样品统计显示,山西组砂岩类型以岩屑砂岩、石英砂岩为主,其次为岩屑石英砂岩,盒8段砂岩主要为岩屑砂岩、岩屑石英砂岩;不同类型的砂岩物性不同,石英砂岩物性最好,其次是岩屑石英砂岩,岩屑砂岩物性最差;沉积相分析认为,子洲地区山西组主要为三角洲前缘,盒8段发育辫状河三角洲前缘,探明了重点气层组山23及盒82沉积微相的平面展布。结论有利的天然气储集体主要形成于水下分流河道微相,由于山西组和盒8段形成时古地理条件的不同,其砂体类型及储集性能也有一定的差异。     

Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937

The surface expression of HIV-1 coreceptors (CXCR4 and CCR5) on monocytes can be regulated by the ligand of CD14, and the susceptibility of the cells to HIV-1 is then changed. Our previous study found that monoclonal antibody against CD14 could dramatically inhibit CXCR4-mediated chemotaxis and cell-cell fusion. Based on these studies, we explored potential relationship between CD14 and CXCR4 on monocytic cell line U937. Flow cytometry analysis showed that anti-CXCR4 monoclonal antibody (mAb) 12G5 strongly inhibited binding of the FITC-conjugated anti-CD14 monoclonal antibodies (TUK4 and UCHM1) to U937, while another CXCR4-specific mAb B-R24 did not show any effect on this binding. On the other hand, two anti-CD14 monoclonal antibodies (TUK4 and UCH-M1) obviously inhibited the binding of the PE-conjugated anti-CXCR4 mAb 12G5 to U937 but did not inhibit the binding of mAb 12G5 to CXCR4-transfected 3T3 cells (3T3.T4.CXCR4), which indicates that the blocking of mAb 12G5 binding to CXCR4 by CD14-specific mAbs is not involved in the possibility that CD14-specific mAbs directly bind to CXCR4. These results suggested existence of a close association between CD14 and CXCR4 on monocytic cell line U937.     

Synthesis, Characterization and Calorimetry of Ni（C4H8N2O3）2Cl2

A coordination complex was synthesized from NiCl2 and dipeptide glycylglycine（GG）. It was characterized by element analysis, NMR and TG methods, and then was determined to be Ni（C4HsN2O3）2Cl2. Using an isoperibolic reaction calorimeter, the standard molar enthalpy of formation of Ni（GG）2Cl2（solid） has been determined to be -（1 674.66±2.02） kJ · mol^-1 at 298.15 K.     

CD40L对B淋巴细胞表面分子及分泌IL-12的影响

在体外使用CD40L作用于B淋巴细胞使之活化,通过测定B淋巴细胞表面分子及分泌细胞因子的变化,探讨B淋巴细胞作为抗原递呈细胞在抗肿瘤免疫中的优势,为B淋巴细胞特异性活化细胞毒T淋巴细胞(cytotoxic T lymphocyte,CTL)进而发挥其肿瘤细胞杀伤效应奠定基础.取7名健康成人外周静脉血,经淋巴细胞分离液分离人外周血单个核细胞(PBMC),分为对照组和实验组,实验组培养液中加入加入CD40L和IL-4,共培养21 d.观察B淋巴细胞生长状况,并在第7 d、第14 d和第21 d,用流式细胞仪(FCAS)测定其表面分子CD80、CD86和CD19,在第21 d,使用酶联免疫吸附法(ELISA)测定培养液中的IL-12水平.结果显示,在CD40L的作用下,B淋巴细胞呈克隆样增殖,并高表达表面分子CD80/CD86和CD19,和对照组相比,B淋巴细胞分泌IL-12水平升高(P＜0.05).上述实验结果说明通过CD40L的刺激,B淋巴细胞得到了活化,提示通过该途径活化的B淋巴细胞具备了作为抗原递呈细胞而发挥其抗肿瘤免疫治疗的能力.     

英语专业八级考试人文知识题评析

本文从信度、效度及反拨效应等几个方面对2005—2009年英语专业八级考试新增人文知识实考试题进行了简要评析，希望对提高英语专业八级考试人文知识试题的质量有一定的实际价值，以及对考试相关人员有一定的启示。     

3Cr2W8V钢热应力疲劳探讨

本文研究了热处理温度和循环温度对3Cr2W8V钢热疲劳抗力的影响,探讨了热疲劳抗力与常规力学性质之间的联系.