离子交换色谱流动相组成对核糖核酸酶的折叠影响

用离子交换色谱法（IEC）研究了还原变性核糖核酸酶（RNase A）折叠过程中，复性缓冲液种类及pH值、流动相中脲浓度及盐种类对还原变性核糖核酸酶复性的影响。发现pH为弱碱性，用PBS作为缓冲液能提高复性效率；当流动相中含有2．0mol／L脲时复性产率较高；洗脱剂用NaCl时活性回收率较高。     

Offlattice Model in the Prediction of Protein 3D Structure

3-dimension HPNX offiattice model is developed from the 2-dimension HP offiattice model. In the HP model, 20 types of amino acid monomers are divided into two classes, H （non-polar monomer） and P （polar monomer）. In the HPNX model, polar monomers are split into positively charged （P）, negatively charged （N） and neutral （X） monomers. A new evolutionary algorithm is applied to study long chains of the HPNX offiattice protein model. This method successfully predict the structures of several proteins in the 3-dimension space that are similar to the structures gotten by X-Ray Crystallography and NMR and published in the PDB（Protein Data Bank）.     

管状非绝热反应中的燕尾型突变

考虑管状非绝热反应的轴向弥散定常问题，提出了求解相应于燕尾型突变的四阶折叠点的正则扩张系统，最后用一个有效的算法找到了管状非绝热反应问题中的燕尾型突变点。     

求解蛋白质折叠问题的拟人算法: 对PERM的改进

PERM(Pruned-Enriched-Rosenbluth Method)是目前文献中依格点模型求解蛋白质折叠问题的最高效算法. 给出了PERM算法的一种拟人解释, 对算法中的权重及预测值进行了拟人化的改进, 并对选择动作时不同情况下的权重计算公式进行了统一. 综合这些策略得到了改进的PERM算法——人口控制算法. 该算法在计算效率上有了明显的提高: 对当前文献中公认的最难的4个算例的计算都达到了最优解, 计算速度较PERM提高了几倍至几百倍. 对于这4个难例中的3个, 还找到了迄今为止文献中所没有的全新的最低能量构形.     

Respective roles of short-and long-range interactions in protein folding

A new method was presented to discuss the respective roles of short- and long-range interactions in protein folding. It‘s based on an off-lattice model, which is also being called as toy model. Simulated annealing algorithm was used to search its native conformation. When it is applied to analysis proteins lagt and laho, we find that helical segment catmot fold into native conformation without the influence of long-range interactions. That‘s to say that long-range interactions are the main determinants in protein folding.     

Investigation of Highly Designable Dented Structures in HP Model with Hydrogen Bond Energy

Some highly designable protein structures have dented on the surface of their native structures, and are not full compactly folded. According to hydrophobic-polar （HP） model the most designable structures are full compactly folded. To investigate the designability of the dented structures, we introduce the hydrogen bond energy in the secondary structures by using the secondary-structure-favored HP model proposed by Ou-yang etc. The result shows that the average designability increases with the strength of the hydrogen bond. The designabilities of the structures with same dented shape increase exponentially with the number of secondary structure sites. The dented structures can have the highest designabilities for a certain value of hydrogen bond energy density.     

基于低电源电压混频器的设计

提出了一种适用于低电源电压应用的混频器,其核心部分采用开关跨导形式,使得开关器件导通时的有限开态电阻引起的电压降减小到零,并在输出端采用折叠级联输出,降低了负载电阻引起的直流电压降,达到了在低电源电压下应用的目的.在1.3 V的电源电压下,电路仿真结果显示:转换增益为-11.5 dB,噪声系数为20.648 dBm,1 dB压缩点为-5.764 dBm,三阶交调失真点为4.807 dBm.     

对称化L2-偏差下组合设计的均匀性

基于对称化L2-偏差讨论了组合设计的均匀性,在两种特殊的情形下分别得到了两水平部分因子设计的组合设计的离散偏差的下界,数值例子说明这些下界是紧的,因此这些下界可以作为寻找最优折叠反转方案的基准。     

Design and application of new folding support mathods without landing in the construction of highway bent cap

文章以铜黄高速公路常家河盖梁支架为例,详细介绍了新型折叠式无落地支架的设计与应用.