粘滞耗能支撑框架弹塑性地震时程分析

利用粘滞阻尼器对工程结构进行减震是耗能减震方法中最有效的新型抗震技术之一。结构设计中通常是粘滞阻尼器与钢支撑串联形成粘滞耗能支撑，并将其附设于结构层框架柱之间。粘滞耗能支撑具有显著的耗能特性，可有效地减小建筑物的地震反应。本文结合一采用钢支撑进行抗震加固改造的工程实例，通过粘滞耗能支撑设计对原结构进行耗能减震研究，并进行了粘滞耗能支撑结构的弹塑性地震反应动力时程分析。     

TRIZ理论研究综述

系统阐述了TRIZ的发展、工程冲突解决表、发明问题的级别、物质-场(S-场)分析、发明问题解决算法(ARIZ)和技术系统的演化。并分析了国外近几十年来在该方面的研究进展，提出了TRIZ的未来发展趋势。     

基于RES理论的岩爆倾向性预测方法

从工程地质因素、复杂环境因素和人为开挖因素3个方面分析了岩爆启动的主要影响因素,在此基础上,提出了一种基于RES理论的岩爆智能预测模型,并论证了人工神经网络的参数分析原理.此外,采用改进的前馈神经网络BP算法对交互作用矩阵进行编码以及对参数的相对交互作用强度进行了分析.研究结果表明:运用该岩爆智能预测模型,不仅使岩爆倾向性的预测具有动态特性,同时又可以方便地对岩爆启动的主控因素进行分析.     

中国近海8种石首鱼类的线粒体16S rRNA基因序列变异及其分子系统进化

通过PCR扩增出中国近海石首鱼科(Sciaenidae)6属8个代表种的线粒体16S rRNA基因,纯化后直接测序.利用多个生物软件对序列变异和碱基组成进行分析,计算Kimura 2-parameter遗传距离、平均核苷酸变异数、平均每位点核苷酸替代数等遗传信息指数,并结合GenBank上大斑石鲈(Pomadasys maculates)同源序列构建UPGMA,NJ,ME和MP系统树.结果表明,叫姑鱼(Johnius belengerii)为最早分化的一枝,其次为黄姑鱼(Nibea albiflora)和白姑鱼(Argyrosomus argentatus),而黄鱼亚科(包括大黄鱼(Pseudosciaena crocea)、小黄鱼(Pseudosciaena polyactis)、棘头梅童鱼(Collichthys lucidus)、黑鳃梅童鱼(Collichthys niveatus)和鮸鱼(Miichthys miiuy))分化最晚,这支持了形态学得出的结论.在分子水平上明确了叫姑鱼亚科和黄鱼亚科的系统进化地位,并得出黄姑鱼比白姑鱼更早分化的新推论,但对于形态学上将白姑鱼和黄姑鱼归属于同一个亚科的结论还有待使用多个不同进化速率的基因加以分析考证.为探讨中国石首鱼类分子系统进化做了尝试,并就线粒体16S rRNA基因在该科鱼类系统进化研究的应用潜力进行了剖析.     

Spindlin 1编码的蛋白质定位于细胞核并使NIH3T3细胞发生恶性转化

研究了卵巢癌中高表达的新基因spindlin1的亚细胞定位及其对小鼠NIH3T3细胞生物学行为的影响, 并探讨了人spindlin1基因的生物学功能. 采用脂质体转染法将构建的融合蛋白表达载体pEGFP-N1/ pEGFP-N1-spindlin1瞬时转染COS-7细胞,观察spindlin1基因的亚细胞定位;同时稳定转染NIH3T3细胞,经G418抗性筛选后,用RT-PCR筛选并鉴定表达spindlin1的稳定细胞株;通过细胞增殖活性、流式细胞检测、软琼脂克隆形成、迁移实验及裸鼠成瘤等对转染细胞的生物学行为进行检测. 结果表明,spindlin1定位于胞核;并促进NIH3T3细胞的增殖,使其处于G2/M期的细胞比例显著增加;同时证实过表达spindlin1的NIH3T3细胞不仅具明显的克隆形成及迁移能力,而且能使裸鼠成瘤. 由此我们得出结论:spindlin1基因过表达可促使NIH3T3细胞发生恶性转化,提示spindlin1可能是一种与肿瘤形成相关的原癌基因.     

方型传力杆在水泥混凝土路面施工中的应用

本文简单的介绍了水泥混凝土路面工程常用的传力杆采用圆面形式，但实际施工时存在一些问题，如改为方形传力杆则能更好地解决存在的问题。     

岩土计算力学发展动向

第5届国际固体材料断裂与强度会议(The 5th International Conference on Fracture and Strength of Solids)于2003年10月19～23日在日本仙台召开。会议主办单位是日本的东京工业大学和东北大学。参加本次大会的人员来自30多个国家，收到论文300余篇，为历届参会人数、发表论文数之最。我国约有50余人参加，我校应邀参会人员2名，宣读论文6篇。     

图片报道

文山州境内有文山县老君山等八个省、州级自然保护区，据初步统计，在八个保护区内共有国家重点保护植物46科73种，下面介绍其中的几种珍稀植物。     

Viola baoshanensis, a plant that hyperaccumulates cadmium

This paper reports upon the finding of Viola baoshanensis, a cadmium (Cd) hyperaccumulator through field survey and greenhouse experiments. Average Cd concentration in the shoots and roots of V. baoshanensis growing on Baoshan lead/zinc mine in Hunan Province, China, was 1168 and 981 mg/kg, respectively, varying from 456 to 2310mg/kg in the shoots, and from 233 to 1846 mg/kg in the roots.The ratio of Cd concentration in shoot to root (DW) and that in plant shoots to total concentration in surface soil was 1.32 and 2.38, respectively. Under nutrient solution culture, biomass (DW) of V. baoshanensis exposed to 0-30 mg/L Cd insolution increased with Cd supply increasing and reached a maximum at 30 mg/L Cd. Further increase of Cd concentration (40, 50 mg/L) in solution significantly reduced biomass.Cd concentration in the shoots was positively correlated with Cd concentration in the culture medium. Cd concentration in the shoots reached 4825 mg/kg at 50 mg/L Cd solution. Theratio of Cd concentration of shoots to roots in V. baoshanensis was greater than 1 at all Cd treatments, with an average of 1.67 (1.14-2.22). The results indicate that V. baoshanensis is a Cd hyperaccumulator plant.     

RNA interference-mediated inhibition of Hepatitis B Virus replication

Persistent and recurrent infection of hepatitis B virus (HBV) represents one of the most common and severe viral infections of humans, and has caused a formidable health problem in the affected countries. Currently used antiviral drugs have a very limited success on controlling HBV replication and infection. RNA interference (RNAi), a process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of target mRNA in mammalian and plant cells, has recently been used to knockdown gene expression in various species. In this study, we sought to determine whether RNAi-mediated silencing of HBV viral gene expression could lead to the effective inhibition of HBV replication. We first developed RNAi vectors that expressed small interfering RNA (siRNA) and targeted the HBV core or surface gene sequence. Our results demonstrated that these specific siRNAs efficiently reduced the levels of corresponding viral RNAs and proteins, and thus suppressed viral replication. Treatment with siRNA gave the greatest reduction in the levels of HBsAg (92%) and in HBeAg (85%) respectively in the cultured cell medium. Our findings further demonstrated that the RNAi-mediated antiviral effect was sequence-specific and dose-dependent. Therefore, our findings strongly suggest that RNAi-mediated silencing of HBV viral genes could effectively inhibit the replication of HBV, hence RNAi-based strategy should be further explored as a more efficacious antiviral therapy of HBV infection.