Effect of serum on PEGylated quantum dots: Cellular uptake and intracellular distribution

Protein adsorption is closely related with the interactions between nanoparticles and physiological systems,and further influences the cellular uptake and distribution of nanoparticles in cells.Although polyethylene glycol(PEG)ylation can largely reduce specific protein adsorption,some protein components in whole serum still interact with nanoparticles.In this work,PEGylated quantum dots(QDs) were used for investigating the quantitative and qualitative relationships of fetal bovine serum(FBS) and the cellular uptake/intracellular distribution in human hepatoma(HepG2) cell line.Nondenaturing polyacrylamide gel electrophoresis and two dimensional electrophoresis were used to analyze the adsorption of protein by PEGylated QDs.Quantitative cellular uptake of PEGylated QDs was determined by fluorescence activated cell sorting(FACS) with different FBS concentrations and incubating durations.The intracellular location of PEGylated QDs in HepG2 cells was observed using a confocal laser scanning microscope(CLSM) and a transmission electron microscope(TEM).This work will be helpful to understand the interaction between nanoparticles and cells with serum.     

基于CA模型的超细晶纯铜退火组织模拟

通过基于静态再结晶(SRX)修正的元胞自动机(CA)模型,模拟了经等通道转角挤压(ECAP)挤压后超细晶纯铜退火过程,探索其微观组织演化规律。超细晶纯铜在不同退火条件下的模拟与实验对比结果表明:相同退火时间后,微观组织平均晶粒尺寸随着退火温度的增加而变大;随着ECAP挤压道次的增加而变小。     

Multi-agent based modeling and simulating for evacuation process in stadium

A multi-agent evacuation model is proposed in this paper to simulate the pedestrian evacuation process in stadium with or without obstacles. The authors give a multi-agent individual decisionmaking framework, in which the action direction of each pedestrian （called agent） is affected by the distance of the agent to the exits and the occupant number and density within the view field of the agent. Different from the existing results, the authors divide all the pedestrians in the stadium into four classes： Young male, young female, old male, and old female. In evacuation process, the weighting that affects individual decision-making between each class of agents is different. In the simulation, the authors present the effects of obstacles, crowd distribution and the exit position in evacuation process. Simulation results show that the proposed model can reproduce exactly the real evacuation process in stadium. Therefore, this method might be useful to assess public buildings design.     

基于细胞自动机的高速密钥产生方法研究

密钥管理是对称密钥加密体制的核心,而密钥产生方法则是密钥管理的基础。介绍了密钥产生的一般方法;根据输入边界细胞自动机的特性提出了一种基于细胞自动机的高速密钥产生方法,并对其统计特性进行了研究。计算机模拟实验表明:基于细胞自动机的高速密钥产生方法不仅具有良好的统计特性(0/1比特的平衡性等),而且具有非体制受限系统的特点。     

Visualization of Gene Mutation Complicated Pattern of Hepatitis B Virus Based on Cellular Automata

Hepatitis B virus shows instantaneous and high rate mutations in biological experiments, some sorts of which affect the efficiency of virus replication greatly through enhancing or depressing the viral replication, while others have no influence at all. Taking advantage of prominent features of cellular automata, we simulate the effect of hepatitis B virus gene mutation on its replication efficiency. The computer simulation results demonstrate the feasibility of our novel model by comparing with the results of biological experiments.     

多层细胞神经网络模型的一般状态解及动力值域估计

针对多层细胞神经网络模型，分析了其一般状态解并对其动力值域作了估计。     

Grey Automata

１ＢａｓｉｃＤｅｆｉｎｉｔｉｏｎｓＬｅｔＵｂｅａｓｅｔ．ＡｇｒｅｙｓｕｂｓｅｔＧｏｆＵｉｓｃｈａｒａｃｔｅｒｉｚｅｄｂｙｔｈｅｆｏｌｏｗｉｎｇｍｅｍｂｅｒｓｈｉｐｆｕｎｃｔｉｏｎ：ｆＧ：Ｕ→［０，１］×［０，１］Ｆｏｒｘ∈Ｕ，ｆＧ（ｘ）＝＜μＧ（ｘ...     

The RCT approach for cell coverage prediction (I): Principles

A new method for the coverage prediction of Cellular Mobile Radio (CMR) system is developed and its basic principle is presented. Unlike the Statistical Prediction Model (SPM) that estimates propagation loss by empirical formulas or the Deterministic Prediction Model (DPM) that reckons propagation loss with analytical expressions, the Radio Computerized Tomography (RCT) method does not seek to express the propagation loss explicitly. The 2-D distribution of propagation loss factor is computed using CT technique, based on measured data collected through drive test under appropriate geometric constraint. The total propagation loss can be obtained by integral of propagation loss factor along the equivalent, ray path. The advantages and limitations of the technique, as well as its implementation are discussed. Biography: Yu Sheng-bing (1974-), male, Ph. D. candidate, research interest: theory analysis and application development of radio propagation in wireless communication systems.     

具分布延时细胞神经网络的指数周期与稳定性

研究了具分布延时细胞神经网络的全局指数周期性与指数稳定性。在没有假设激活函数是有界的、可微的、单调增的情况下,通过应用一些新的分析技巧与Halanay-type不等式方法,得到了确保延时细胞神经网络周期解存在唯一且全局指数周期与全局指数稳定的简单的、容易验证的、新的充分条件。并给出了算例及其仿真结果支持所得结论。     

基于细胞神经网络的牌照倾斜校正及单字切分

车牌自动识别中 ,牌照倾斜和单字切分不准确会使字符识别率下降。为此提出基于细胞神经网络的牌照倾斜校正及单字切分的方法。由阴影检测器得到包含字符的块状区域 ,并得到块状区域的上、下边缘线 ,从而得到牌照的倾斜角度 ,对牌照进行倾斜校正。对校正后的图像 ,用相连单元检测器去除边框及其它干扰 ,并用另一个离散时间细胞神经网络模板完成单字切分。结果表明 ,利用细胞神经网络可以较好地完成牌照倾斜校正和单字切分 ,为后面的字符识别作了较好的准备。