Identification of a novel DNA molecule associated with Tobacco leaf curl virus

A circular DNA molecule, designated as DNAβ, was identified in tobacco plants infected with Tobacco leaf curl virus (TLCV) isolates Y5 and Y8 by PCR using primers based on the conserved region of the two reported DNAβ sequences of whitefly-transmitted geminiviruses (WTGs). The complete nucleotide sequences of DNAβ of Y5 and Y8 (TLCV DNAβ) were determined. Y5 DNAβ comprises 1333 nucleotides encoding 8 predicted ORFs with 4 ORFs in virion-sense DNA and 4 ORFs in complementary-sense DNA; Y8 DNAβ consists of 1338 nucleotides encoding 7 predicted ORFs with 4 ORFs in virion-sense DNA and 3 ORFs in complementary-sense DNA. TLCV DNAβ has little sequence homology to DNA-A of TLCV., except that it shares conserved TAATATTAC loop sequence with TLCV DNA-A. Sequence comparison showed that Y5 DNAβ shared 85% sequence homology with Y8 DNAβ, and both Y5 DNAβ and Y8 DNAβ had relatively low sequence identity (51%–65%) with the reported DNAβ molecules associated with Ageratum yellow vein virus and Cotton leaf curl virus. The immunotrapping PCR and whitefly transmission tests showed that DNAβ molecule could be encapsidated in virus particle and transmitted by Bemisia tabaci. This is the first report of DNAβ associated with WTGs in China.     

Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs

cDNA libraries from aborted human 3-month fetal brain,adult rat and mouse brain were constructed by using a yZAP express cDNA library construction kin.Low molecular weight fragments of the second strand cDNASA were removed by flowing through the Sepharose CL-4B column and the frractionated long,Middle,Short fragments and the combined fragments weire respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus.The 5'ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced.A total of 894 ESTs were obtained and some full-length clones were squenced.By andalyaing the se-quences,12 novel full-length cDNAs were obtained.     

Identification and expression of a gfpK79R mutant of green fluorescent protein

A mutant strain of E.coli showing strong green fluorescence is obtained during the study of cloning and expression of blue fluorescent protein gene bfp. A recombinant plasmid pHN122 has been isolated. It is demonstrated by enzyme digestion, subcloning and sequence analysis that it contains both of bfp and gfpK79R mutant genes. The results of spectrometric analysis show that GFPK79R expressed by pHN122 is the same as the wild type GFP, but its fluorescent intensity is increased 1.25～2.44-fold.     

Rutile U-Pb age for the ultra-high pressure eclogite from the dabie Mountains,central China:Evideence for rapid cooling

The U-Pb isotope compositions of rutile, omphacite and garnet in the eclogite from the Jinheqiao area in the Southern Dabie ultrahigh-pressure metamorphic zone were analyzed. The consistent high precision U-Pb age (218 ±(1.2) Ma of rutile in eclogite from the Dabie Mountains was obtained by two ways of isochron and common Pb correction based on the composition of omphacite. This proves that the omphacite in eclogite has a U/Pb ratio (μ = 2.8) low enough to be used for common Pb correction in the analyses of rutile. Under the rapid cooling condition (40℃/Ma) the closure temperature for U-Pb diffusion in rutile is about 470℃. Thus, this U-Pb age of rutile proves that 218 Ma should be the cooling age of eclogite at 470℃ instead of the peak metamorphic age.     

A possible configuration of H 5 + cluster

On the basis of our previous work, a configuration of H ₅ ⁺ has been presented that is a square center structure, and the energy for the structure of H ₅ ⁺ has been obtained by IACQM. The result showed that there is an extreme energy E_e: −2.45981 [2 Ry] atR = 2.50a ₀. It indicates that the square center structure of H ₅ ⁺ system has the certain stability, which is a possible configuration of H ₅ ⁺ .     

灰色系统理论在无检测器交叉口交通流量预测中的应用

为解决一般预测方法要求原始数据量较大，而无检测器交叉口所能获得的交通流量数据又非常有限的矛盾，提出了利用灰色系统理论预测无检测交叉口交通流通的方法，并建立了一种新的自适应GM（1，1）模型，利用编制的计算机程序对常熟市无检测器交叉口交通流量进行预测计算分析，结果表明自适应GM（1，1）模型可以根据有限的交通流量数据进行预测，且预测精度较之全数据GM（1，1）模型有显著提高，实践证明，该方法是有效的。     

BOT项目"有限追索权"融资方式的风险收益分析

以基础设施建设为背景，针对BOT项目的有限追索权融资方式，建立了贷方和项目公司之间的风险收益分配模型，根据委托代理的理论和思想，分析了贷方和项目公司的风险态度和风险收益的分配问题，得到了使贷方和项目公司的收产佃同时达到最优的收益平衡条件，解决了贷方和项目公司之间的利益冲突问题，验证了BOT融资模式的有效性和可行性。     

用亚硫酸氢钠提高不饱和有机烯酸的产率

对合成山梨酸,肉桂酸,2—呋喃丙烯酸的方法进行了改进:在卤仿反应发生后,酸化前用亚硫酸氢钠去除去过量的次氯酸钠,产物的产率比直接酸化分别提高了21.3%,19.74%,27%,重结晶后纯度达99.05%,99.3%,99.1%。经红外,质谱,碳氢数据分析均符合要求。     

浅谈造山带与沉积盆地的关系

从当今地球科学研究前沿动态出发，论述了造山带与沉积盆地是统一的构造框架和动力体制下形成的孪生体，它们在空间上相互依存，在物质关系上表现为深部的补偿和浅部的剥蚀及直接供给，在它们的形成和演化过程中，深层岩石圈圈层间存在着耦合关系，对今后把造山带和盆地置于一个系统加以研究，具有重要的指导意义。     

整流后倒U型管内气液两相流动的稳定性及流型分布

在倒U型管垂直段的两端内分别放置一个整流环，来消除入口弯头对垂直段流动的影响，通过对单相流体在倒U型管内的流动进行数值计算，以及对整流作用下倒U型管内气液流动的稳定性和流型的分布进行实验研究，发现整流环的整流作用可以增强管道内流动的稳定性，实验还发现，倒U型管垂直上升段内的间歇性流动（弹状流和块状流）区域减小，环状流区域增大，流动的稳定性增强。