噬菌体展示禽流感病毒抗原变异性基因片段文库的构建

构建T7噬菌体展示禽流感病毒抗原变异性基因片段文库. 首先， 从Gene Bank中查找筛选禽流感病毒抗原变异性基因, 将其截短、 修饰、 简并后得到禽流感病毒抗原变异性基因微阵列. 其次， 将合成的禽流感病毒抗原变异性基因片段文库扩增、 酶切, 链接到双酶切后的T7噬菌体载体基因上, 构成重组噬菌体DNA. 最后， 重组噬菌体DNA经体外包装和扩增, 得到T7噬菌体展示文库, 并进行T7噬菌体展示文库滴度、 重组率和免疫活性测定. 实验结果表明, 从Gene Bank中查找、 筛选、 剪切和修饰共获得96 258条序列构建T7噬菌体展示文库, 原始文库滴度为3.6×107个菌落/mL, 重组率大于90%. 用禽流感病毒H5N1抗体进行捕获, 经聚合酶链式反应(PCR)鉴定, 得到理想目的条带, 证明噬菌体表面展示蛋白具有抗原活性, 可用于禽流感病毒感染患者的快速检测及抗原表位筛选.     

ON THE COLLISION DETECTION PROBLEM OF TWO MOVING OBJECTS DESCRIBED BY ALGEBRAIC SETS

In this paper, the collision problem of two moving objects is investigated. The objects are described by two algebraic sets （ellipses or circles in the paper）. The collision problem discussed involves both static and dynamic case. The static case is that each object moves with known velocity. We use nonlinear programming to decide whether the objects collide. The dynamic case is that each object is controlled by a constraint external force which can be regulated online. For the dynamic case, the collision problem can be modelled as a Minmax problem which can be solved by using differential games. If collision occurs, the time and place of the first collision are given. The moving trajectories are provided in the paper.     

优化矿井巷道 提高经济效益

通过讨论矿井回风大巷断面与矿井生产营运成本之间的内在联系,提出一种优化设计矿井回风大巷巷道断面的途径。     

安徽省豹猫的分布和数量

应用皮张收购资料的统计和访问调查、野外实地考察等方法，研究了豹猫在安徽省的分布、数量及历史变化。     

Structure of astacin and implications for activation of astacins and zinc-ligation of collagenases.

Astacin, a digestive zinc-endopeptidase from the crayfish Astacus astacus L., is the prototype for the 'astacin family', which includes mammalian metallo-endopeptidases and developmentally regulated proteins of man, fruitfly, frog and sea urchin. Here we report the X-ray crystal structure of astacin, which reveals a deep active-site cleft, with the zinc at its bottom ligated by three histidines, a water molecule and a more remote tyrosine. The third histidine (His 102) forms part of a consensus sequence, shared not only by the members of the astacin family, but also by otherwise sequentially unrelated proteinases, such as vertebrate collagenases. It may therefore represent the elusive 'third' zinc ligand in these enzymes. The amino terminus of astacin is buried forming an internal salt-bridge with Glu 103, adjacent to His 102. Astacin pro-forms extended at the N terminus, as observed for some 'latent' mammalian astacin homologues, did not exhibit this 'active' conformation, indicating an activation mechanism reminiscent of trypsin-like serine proteinases.     

浅谈液压防爆绞车的发展

通过对国内外液压防爆绞车的技术水平及其现状的分析,探讨了液压防爆绞车的发展前景。     

冠心病患者拔牙前后的内科保障

冠心病患者拔牙有一定的危险性,不但要严格掌握拔牙适应症,还要采取系统的内科保障,确保生命安全本报告有120例冠心病患者临床资料,介绍冠心病患者的拔牙指征和常用的内科治疗措施.     

固化盐渍土的自生体积稳定性

为避免盐渍土在固化过程中可能发生的体积变形危害,研究其自生体积稳定性。制备了290mm长的试件,利用高精度位移测量系统,测试了不同含量的氯盐与硫酸盐在不同固化剂体系下引起的固化土的线性膨胀/收缩率,分析了C a2 、N a 以及含水率对膨胀/收缩率的影响。结果表明:固化氯盐盐渍土不会发生较大的体积变形,而固化硫酸盐盐渍土可能引起巨大的膨胀率;采用矿渣-粉煤灰-水泥复合固化剂,参与化学反应而产生膨胀性产物的C a2 减少,可以降低固化盐渍土的膨胀率。当C a2 不足而N a 充分时,氯盐和硫酸盐不会对化学反应起促进作用。含水率会影响固化土结构的疏密程度以及膨胀性产物的生长位置。     

解放CA-141型汽车最优二级维护周期研究

结合CA－141型汽车运行生产实际，在计划预防方针指导下，借助于可靠性理论、更新理论、优化理论等，建立确定汽车最优维护周期的数学模型．并具体求出CA－141型汽车的最优二级维护周期里程．本文提供方法亦可应用于其它国产和进口汽车最优维护周期的确定。     

人工气候条件下的人体着装试验的研究

本文通过物理试验方法对棉、毛、丝、棉麻及毛涤五种面料进行性能测试,得出其风格数值、透湿阻抗.为了反映面料对人的生理、心理的影响,有必要在人工气候条件下进行人体模拟着装试验,得出面料的人体舒适感觉值。借助多元线性回归方法,通过物理测试与人体试验的对照分析,发现它们之间具有良好的一致性。