重组GATA4腺病毒的构建及心肌细胞感染

利用AdEasy腺病毒表达系统构建了GATA4基因过表达腺病毒.编码大鼠GATA4基因的目的片段克隆入pAdTrackcmv质粒,pAdTtrackcmv-GATA4穿梭质粒经PmeⅠ线性化后转入含pAdEasy-1的BJ5183菌进行同源重组.pAdEsay-GATA4重组质粒经卡那霉素抗性筛选及PacⅠ酶切鉴定.pAdEsay-GATA4转入293A细胞进行包装,产生具有感染性的重组病毒.Ad-GATA4重组病毒感染HeLa及乳鼠心肌细胞,通过免疫印迹及实时定量聚合酶链反应(Real-time PCR)检测GATA4表达及促心肌肥大效应.Ad-GATA4重组病毒感染乳鼠心肌细胞后,诱导心肌细胞表面积明显增加,ANF表达明显增强.结果表明,Ad-GATA4腺病毒成功感染心肌细胞并诱导了大鼠心肌肥大表型的出现.     

Effects of mechanical strain amplitude on the isothermal fatigue behavior of H13

Isothermal fatigue (IF) tests were performed on H13 tool steel subjected to three different mechanical strain amplitudes at a constant temperature to determine the effects of mechanical strain amplitude on the microstructure of the steel samples. The samples' extent of damage after IF tests was compared by observation of their cracks and calculation of their damage parameters. Optical microscopy (OM) and scanning electron microscopy (SEM) were used to observe the microstructure of the samples. Cracks were observed to initiate at the surface because the strains and stresses there were the largest during thermal cycling. Mechanical strain accelerated the damage and softening of the steel. A larger mechanical strain caused greater deformation of the steel, which made the precipitated carbides easier to gather and grow along the deformation direction, possibly resulting in softening of the material or the initiation of cracks.     

浅析驾驶适宜性检测对驾驶培训的重要性

事故统计分析表明,新驾驶员为事故多发群,由此可见我国的驾驶培训工作存在一定缺陷。通过对驾驶培训内容分析,发现驾驶员对驾驶工作还存在适宜性问题。驾驶适宜性检测项目对安全行车非常必要,对提高培训质量和驾驶员队伍素质极其重要。     

工业现场LED数据显示屏系统的开发

分析工业现场LED数据显示屏主要是将各种类型传感器采集的数据，提出利用数据采集转换芯片，或者利用接口传送到LED显示屏上，进行数据的实时显示方案。     

存款准备金率变动对股市影响的实证研究

运用VAR模型的G ranger因果检验和脉冲响应函数分析研究了中央银行存款准备金率变动对沪深300指数的影响,并通过方差分解分析前者对后者的贡献度。得出的结论是存款准备金率变动对股市波动在短期内存在一定负方向的影响,股市波动对存款准备金率则无影响。但在长期内,存款准备金率变动对股市影响很小。最后在此基础上提出相关的货币政策的建议。     

Biomimetic fluorapatite films for conservation of historic calcareous stones

Fluorapatite protective films were prepared on marble substrates using a biomimetic method. By mimicking the mineralization mechanism of enamel, phosphorus and fluorine were introduced on the surface of the marble substrate. In the presence of a biological template, namely collagen, an integrated fluorapatite film was produced and the marble substrate was entirely covered. The prepared fluorapatite films were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray (EDX) spectroscopy. The performances of the fluorapatite films were evaluated by color changes, capillary water absorption, and acid resistance tests. The results revealed that the fluorapatite films had good compatibility with the marble substrate; the physical properties such as color and capillary water adsorption of the marble substrates were unchanged. The fluorapatite films also had good acid resistance and were stable even in heavy acid rain.     

伸缩臂叉装车变幅机构的铰点位置优化

鉴于伸缩臂叉装车变幅机构的铰点位置的确定直接影响变幅液压缸的负载值和压力冲击,分析了变幅机构的工作特性,以提高传动力比和降低油压波动值为目标,采用统一目标函数法建立多目标优化设计数学模型,利用Matlab遗传算法工具箱对变幅机构的铰点位置进行优化.结果表明优化后的尺寸能大大提高叉装车的变幅机构的作业性能.     

新时期煤炭企业矿山管理新思路浅析

结合当前国内煤炭市场形势及国家煤炭企业综改政策，对新时期煤炭企业矿山管理的安全管理和经营管理等方面提出了创新性思考和可行性建议。     

广西风电场选址重点区域分析

广西北部处于云贵高原东南边缘,南部濒临北部湾,因而形成了广西的特色气候.文章通过分析风速的影响因素,结合广西气候特征,提出在广西能够形成较好风能资源的地形特征,并提出广西风电场选址的三类重点区域,为风电场选址提供依据.     

心理技术的哲学反思

对心理技术的哲学反思包括三个方面的内涵:从心理技术的在场性、心理场域的作用及其对身体映像的控制来分析心理技术的实体性存在;从知识建构、知识扩展以及思维的形成等方面可以为认识论反思提供启示;从心理技术的研究、心理技术的实施以及心理技术与道德的涉身性了解心理技术的道德困境。