Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

乌鲁木齐市现代化水平现状评价

基于前人的理论与经验 ,本文建立了乌鲁木齐现代化指标体系和标准 ,以 1998～ 2 0 0 0年统计数据为例 ,分析了乌鲁木齐现代化实现现状 ,根据其解析评价了乌鲁木齐现代化建设中主要存在的问题 ,进而提出相应的对策。     

压电材料非线性本构关系及有限元实施

通过对试验结果的分析 ,提出经过简化的压电陶瓷PZT 4的非线性本构关系 ,建立相应的有限元计算方法 .以多重复合式压电材料启动器为例 ,计算电荷载作用下的电畴翻转、反向极化和反向电致屈服等非线性响应 ,从而对压电材料启动器的工作特性有所了解 ,并对它的破坏机理的研究提供一些启示 .     

新型电液激振装置的性能研究

结合电气与液压传动的性能优势，开发出电液激振装置，推导出该装置相关参数的相互关系式。经试验得出，激振装置的振动频率、振幅、压强、流量可以根据需要进行调节。可成为煤矿、冶金、建筑等行业振动设备的一种新型激振装置。     

用知识管理构建电子商务实验教学体系

文章提出用知识管理构建电子商务实验教学体系的观点，就电子商务实验教学体系的建设中，如何构建学习组织、学习系统的思想和方法，进行知识交流与共享．运用知识管理进行实验创新，进行了相应的探讨．     

应用于加速器驱动次临界系统的程序开发

开发了应用输运理论方法的两套程序系统：蒙特卡罗－燃耗程序系统和输运－燃耗程序系统。针对蒙特卡罗程序不能计算燃料的同位素成分变化，提出将它和燃耗计算程序耦合以完成燃耗计算。燃耗程序所使用的单群截面由蒙特卡罗程序计算。将用于常规反应堆的计算方法和策略推广到输运－燃耗程序系统，这使得计算步序大大简化，并改进了精度。对加速器驱动次临界系统的基准题进行了校核计算，其结果和其他国家的计算结果符合良好，这表明所提出的计算方法与程序系统是成功的。     

China'''' s Fundamental Research in Energy Utilizations and Environment

The progress in the science of energy utilizations will act crucial effect on the developments of energy science and technology, which will then promote social and economical developments and fulfill requirements for the national strategic objectives. For the sake of sustainable development, a harmonious blend of energy utilizations and environment considerations will become one of the vital topics in the future research area of energy science. It is suggested that clean and high-efficiency utilization of traditional or fossil energy resources, fundamental investigations on the energy and environment theory, renewable energy utilizations, and the development of nuclear energy are selected as priority research areas during the period of the Tenth Five-year Plan of China, according to the development trend of the world energy science and the research background of Chinese energy science. It is expected to promote the interdisciplinary investigations in the science of energy u-tilizations and provide scien     

西部地区科技创新现状及对策研究

本文分析了国内外科技创新形势，对西部地区，特别是陕西、四川、重庆区域的科技创新能力进行重点研究，通过对比指出西部科技创新中所存在的体制、机制方面的问题，提出了西部地区加强科技创新促进经济发展的若干对策。     

桩端压力注浆桩在卵石层中的应用

灌注桩采用桩端压力灌浆新技术后，能大幅度地提高基桩承裁能力，减小其沉降，在工程中已运用多年，但大量工程实践表明其对不同地层的适应性是不同的。根据某工程中钻孔灌注桩现场试验，对桩端压力灌浆技术在卵石层中灌注桩的运用效果其及经济、社会效益进行了分析。